Supplementary information: The self-association equilibrium of DNAJA2 regulates its interaction with unfolded substrate proteins and with Hsc70
Contiene: Supplementary Figure 1. Domain organization of DNAJA2 and the variants used in this study.-- Supplementary Figure 2. Prediction of disordered regions in DNAJA2.-- Supplementary Figure 3. Negative staining microscopy images of the samples analysed in this work.-- Supplementary Figure 4. Arc...
| Authors: | , , , , , , , , , , , |
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| Format: | conjunto de datos |
| Status: | Published version |
| Publication Date: | 2023 |
| Country: | España |
| Institution: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repository: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/346957 |
| Online Access: | http://hdl.handle.net/10261/346957 |
| Access Level: | Open access |
| Summary: | Contiene: Supplementary Figure 1. Domain organization of DNAJA2 and the variants used in this study.-- Supplementary Figure 2. Prediction of disordered regions in DNAJA2.-- Supplementary Figure 3. Negative staining microscopy images of the samples analysed in this work.-- Supplementary Figure 4. Architecture of DNAJA2 oligomers.-- Supplementary Figure 5. Workflow of the 3D reconstruction process of DNAJA2wt and DNAJA2ΔG/FR.-- Supplementary Figure 6. Holding activity of DNAJA2wt under conditions that favour the oligomer or the dimer.-- Supplementary Figure 7. Functional characterization of DNAJA2 and its mutants.-- Supplementary Figure 8. DnaJB1 recruits Hsc70 to G6PDH aggregates more efficiently than DNAJA2.-- Supplementary Figure 9. Binding of Hsc70 to DNAJA2 or DNAJA2QPN assemblies followed by chaperone labelling.-- Supplementary Table 1. Data collection parameters for DNAJA2wt and DNAJA2ΔG/FR.-- Supplementary Table 2. DnaJA2DG/FR model refinement and validation statistics related to Figure 3.-- Supplementary Table 3: Crosslinks detected upon treating DNAJA2wt and DNAJA2DG/FR with BS3, under conditions that favour the oligomer population (low salt concentration, 25 °C).-- Supplementary references |
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