The power of imaging to understand extracellular vesicle biology in vivo.

Extracellular vesicles (EVs) are nano-sized lipid bilayer vesicles released by virtually every cell type. EVs have diverse biological activities, ranging from roles in development and homeostasis to cancer progression, which has spurred the development of EVs as disease biomarkers and drug nanovehic...

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Detalles Bibliográficos
Autores: Verweij, Frederik J, Balaj, Leonora, Boulanger, Chantal M, Carter, David R F, Compeer, Ewoud B, D'Angelo, Gisela, El Andaloussi, Samir, Goetz, Jacky G, Gross, Julia Christina, Hyenne, Vincent, Krämer-Albers, Eva-Maria, Lai, Charles P, Loyer, Xavier, Marki, Alex, Momma, Stefan, Nolte-'t Hoen, Esther N M, Pegtel, D Michiel, Peinado Selgas, Hector, Raposo, Graça, Rilla, Kirsi, Tahara, Hidetoshi, Théry, Clotilde, van Royen, Martin E, Vandenbroucke, Roosmarijn E, Wehman, Ann M, Witwer, Kenneth, Wu, Zhiwei, Wubbolts, Richard, van Niel, Guillaume
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/25961
Acceso en línea:https://hdl.handle.net/20.500.12105/25961
Access Level:acceso abierto
Palabra clave:TRANSFERRIN RECEPTOR
EXOSOME RELEASE
HUMAN-DISEASE
GAG PROTEIN
CELLS
SECRETION
ELEGANS
MICROVESICLES
RNA
DEGRADATION
Descripción
Sumario:Extracellular vesicles (EVs) are nano-sized lipid bilayer vesicles released by virtually every cell type. EVs have diverse biological activities, ranging from roles in development and homeostasis to cancer progression, which has spurred the development of EVs as disease biomarkers and drug nanovehicles. Owing to the small size of EVs, however, most studies have relied on isolation and biochemical analysis of bulk EVs separated from biofluids. Although informative, these approaches do not capture the dynamics of EV release, biodistribution, and other contributions to pathophysiology. Recent advances in live and high-resolution microscopy techniques, combined with innovative EV labeling strategies and reporter systems, provide new tools to study EVs in vivo in their physiological environment and at the single-vesicle level. Here we critically review the latest advances and challenges in EV imaging, and identify urgent, outstanding questions in our quest to unravel EV biology and therapeutic applications.

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