Short oligoalanine helical peptides for supramolecular nanopore assembly and protein cytosolic delivery

In this work we report a rational design strategy for the identification of new peptide prototypes for the non-disruptive supramolecular permeation of membranes and the transport of different macromolecular giant cargos. The approach targets a maximal enhancement of helicity in the presence of membr...

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Autores: Pazo Pascual, Marta, Salluce, Giulia, Lostalé Seijo, Irene, Juanes Carrasco, Marisa, González García, Francisco, García Fandiño, Rebeca, Montenegro García, Javier
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/26917
Acceso en línea:http://hdl.handle.net/10347/26917
Access Level:acceso abierto
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spelling Short oligoalanine helical peptides for supramolecular nanopore assembly and protein cytosolic deliveryPazo Pascual, MartaSalluce, GiuliaLostalé Seijo, IreneJuanes Carrasco, MarisaGonzález García, FranciscoGarcía Fandiño, RebecaMontenegro García, JavierIn this work we report a rational design strategy for the identification of new peptide prototypes for the non-disruptive supramolecular permeation of membranes and the transport of different macromolecular giant cargos. The approach targets a maximal enhancement of helicity in the presence of membranes with sequences bearing the minimal number of cationic and hydrophobic moieties. The here reported folding enhancement in membranes allowed the selective non-lytic translocation of different macromolecular cargos including giant proteins. The transport of different high molecular weight polymers and functional proteins was demonstrated in vesicles and in cells with excellent efficiency and optimal viability. As a proof of concept, functional monoclonal antibodies were transported for the first time into different cell lines and cornea tissues by exploiting the helical control of a short peptide sequence. This work introduces a rational design strategy that can be employed to minimize the number of charges and hydrophobic residues of short peptide carriers to achieve non-destructive transient membrane permeation and transport of different macromoleculesRoyal Society of ChemistryUniversidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades CrónicasUniversidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais MolecularesUniversidade de Santiago de Compostela. Departamento de Química Orgánica20212021-01-0120212021-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10347/26917reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 BES-2015-071779Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PRE2018-085973Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 SAF2017-89890-R PEPTIDOS HIBRIDOS PARA EL TRANSPORTE SELECTIVO Y ENTREGA DE PROTEINAS TERAPEUTICASMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 RYC-2016-20335Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-098795-A-I00 DISEÑO DE AGENTES ANTITUMORALES A PARTIR DE SIMULACIONES DE DINAMICA MOLECULAR, ANALISIS BIG DATA E INTELIGENCIA ARTIFICIAL VALIDADOS POR EXPERIMENTOS BIOFISICOSMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 RYC-2013-13784European Commission http://dx.doi.org/10.13039/501100000780 Horizon 2020 Framework Programme 677786open accesshttp://purl.org/coar/access_right/c_abf2© 2021 The Author(s). Published by the Royal Society of Chemistry. Open Access Article. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licencehttp://creativecommons.org/licenses/by-nc/3.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/269172026-06-15T12:47:27Z
dc.title.none.fl_str_mv Short oligoalanine helical peptides for supramolecular nanopore assembly and protein cytosolic delivery
title Short oligoalanine helical peptides for supramolecular nanopore assembly and protein cytosolic delivery
spellingShingle Short oligoalanine helical peptides for supramolecular nanopore assembly and protein cytosolic delivery
Pazo Pascual, Marta
title_short Short oligoalanine helical peptides for supramolecular nanopore assembly and protein cytosolic delivery
title_full Short oligoalanine helical peptides for supramolecular nanopore assembly and protein cytosolic delivery
title_fullStr Short oligoalanine helical peptides for supramolecular nanopore assembly and protein cytosolic delivery
title_full_unstemmed Short oligoalanine helical peptides for supramolecular nanopore assembly and protein cytosolic delivery
title_sort Short oligoalanine helical peptides for supramolecular nanopore assembly and protein cytosolic delivery
dc.creator.none.fl_str_mv Pazo Pascual, Marta
Salluce, Giulia
Lostalé Seijo, Irene
Juanes Carrasco, Marisa
González García, Francisco
García Fandiño, Rebeca
Montenegro García, Javier
author Pazo Pascual, Marta
author_facet Pazo Pascual, Marta
Salluce, Giulia
Lostalé Seijo, Irene
Juanes Carrasco, Marisa
González García, Francisco
García Fandiño, Rebeca
Montenegro García, Javier
author_role author
author2 Salluce, Giulia
Lostalé Seijo, Irene
Juanes Carrasco, Marisa
González García, Francisco
García Fandiño, Rebeca
Montenegro García, Javier
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas
Universidade de Santiago de Compostela. Centro de Investigación en Química Biolóxica e Materiais Moleculares
Universidade de Santiago de Compostela. Departamento de Química Orgánica
description In this work we report a rational design strategy for the identification of new peptide prototypes for the non-disruptive supramolecular permeation of membranes and the transport of different macromolecular giant cargos. The approach targets a maximal enhancement of helicity in the presence of membranes with sequences bearing the minimal number of cationic and hydrophobic moieties. The here reported folding enhancement in membranes allowed the selective non-lytic translocation of different macromolecular cargos including giant proteins. The transport of different high molecular weight polymers and functional proteins was demonstrated in vesicles and in cells with excellent efficiency and optimal viability. As a proof of concept, functional monoclonal antibodies were transported for the first time into different cell lines and cornea tissues by exploiting the helical control of a short peptide sequence. This work introduces a rational design strategy that can be employed to minimize the number of charges and hydrophobic residues of short peptide carriers to achieve non-destructive transient membrane permeation and transport of different macromolecules
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10347/26917
url http://hdl.handle.net/10347/26917
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 BES-2015-071779
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PRE2018-085973
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 SAF2017-89890-R PEPTIDOS HIBRIDOS PARA EL TRANSPORTE SELECTIVO Y ENTREGA DE PROTEINAS TERAPEUTICAS
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 RYC-2016-20335
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-098795-A-I00 DISEÑO DE AGENTES ANTITUMORALES A PARTIR DE SIMULACIONES DE DINAMICA MOLECULAR, ANALISIS BIG DATA E INTELIGENCIA ARTIFICIAL VALIDADOS POR EXPERIMENTOS BIOFISICOS
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 RYC-2013-13784
European Commission http://dx.doi.org/10.13039/501100000780 Horizon 2020 Framework Programme 677786
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by-nc/3.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by-nc/3.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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