LC-MS identification of oligopeptides bound to AmiA and AliB during production and purification [Dataset]

(A) The plots show the frequency distribution of peptide lengths that are found bound to AmiA (left panel) or AliB (right panel). The data are represented as mean ± SE calculated from non-linear Gaussian fit version (full width at half maximum, FWHM) employing the function: (B) Sequence logo analysi...

Descripción completa

Detalles Bibliográficos
Autores: Alcorlo, Martín, Abdullah, Mohammed R., Steil, Leif, Sotomayor, Francisco, López de Oro, Laura, Castro, Sonia de, Velázquez, Sonsoles, Kohler, Thomas P., Jiménez, Elisabet, Medina, Ana, Usón, Isabel, Keller, Lance E., Bradshaw, Jessica L., McDaniel, Larry S., Camarasa Rius, María José, Völker, Uwe, Hammerschmidt, Sven, Hermoso, Juan A.
Tipo de recurso: conjunto de datos
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/374765
Acceso en línea:http://hdl.handle.net/10261/374765
Access Level:acceso abierto
Palabra clave:Unexpected remarkable promiscuity
Streptococcus pneumoniae </
Produced de novo
Multiple crystallographic structures
Escherichia coli </
Diverse peptide specificities
Closed conformations along
Certain amino acids
>, displaying affinity
Ensure sufficient uptake
Mass spectrometry analysis
div >< p
Orchestrating oligopeptide uptake
Four proteins building
Ami transporter system
Abc transporter channel
Pneumococci
Structural analysis
Oligopeptide recognition
Oligopeptide binding
Uptake mechanism
Wide range
Vivo implications
Transport systems
Substantial array
Structural basis
Silico modelling
Shedding light
Oligopeptides demonstrates
Invasive infections
Energy balance
Cellular cytoplasm
Cell surface
Binding cassette
Becomes indispensable
Auxotrophic nature
Abc transporters
Descripción
Sumario:(A) The plots show the frequency distribution of peptide lengths that are found bound to AmiA (left panel) or AliB (right panel). The data are represented as mean ± SE calculated from non-linear Gaussian fit version (full width at half maximum, FWHM) employing the function: (B) Sequence logo analysis of the peptides bound to AmiA (left panel) or AliB (right panel). The sequence logos were generated based on the alignment of the identified peptides bound to each respective protein, taking just into account their starting positions for the alignment. These plots were generated using the WebLogo server (https://weblogo.berkeley.edu/logo.cgi) [29]. (C) Sector graphs comparing the identified bound peptides to AmiA (shown in the upper panel) and AliB (shown in the lower panel). The peptides were categorized according to the biological function exerted by the protein from which they originated.

Similares en LA Referencia