Functional analysis of saccharomyces cerevisiae ribosomal protein Rpl3p in ribosome synthesis

Ribosome synthesis in eukaryotes requires a multitude oftrans-acting factors. These factors act at many steps as the pre-ribosomal particles travel from the nucleolus to the cytoplasm. In contrast to the well-studiedtrans-acting factors, little is known about the contribution of the ribosomal protei...

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Autores: Valle Rosado, Iván, Kressler, Dieter, Cruz Díaz, Jesús de la
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2007
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/44309
Acceso en línea:http://hdl.handle.net/11441/44309
https://doi.org/10.1093/nar/gkm388
Access Level:acceso abierto
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spelling Functional analysis of saccharomyces cerevisiae ribosomal protein Rpl3p in ribosome synthesisValle Rosado, IvánKressler, DieterCruz Díaz, Jesús de laRibosome synthesis in eukaryotes requires a multitude oftrans-acting factors. These factors act at many steps as the pre-ribosomal particles travel from the nucleolus to the cytoplasm. In contrast to the well-studiedtrans-acting factors, little is known about the contribution of the ribosomal proteins to ribosome biogenesis. Herein, we have analysed the role of ribosomal protein Rpl3p in 60S ribosomal subunit biogenesis. In vivo depletion of Rpl3p results in a deficit in 60S ribosomal subunits and the appearance of half-mer polysomes. This phenotype is likely due to the instability of early and intermediate pre-ribosomal particles, as evidenced by the low steady-state levels of 27SA3, 27SBSand 7SL/S precursors. Furthermore, depletion of Rpl3p impairs the nucleocytoplasmic export of pre-60S ribosomal particles. Interestingly, flow cytometry analysis indicates that Rpl3p-depleted cells arrest in the G1 phase. Altogether, we suggest that upon depletion of Rpl3p, early assembly of 60S ribosomal subunits is aborted and subsequent steps during their maturation and export preventedOxford University PressGenética2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/44309https://doi.org/10.1093/nar/gkm388reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésNucleic Acids Research, 35 (12), 4203-4213.10.1093/nar/gkm388info:eu-repo/semantics/openAccessoai:idus.us.es:11441/443092026-06-17T12:51:07Z
dc.title.none.fl_str_mv Functional analysis of saccharomyces cerevisiae ribosomal protein Rpl3p in ribosome synthesis
title Functional analysis of saccharomyces cerevisiae ribosomal protein Rpl3p in ribosome synthesis
spellingShingle Functional analysis of saccharomyces cerevisiae ribosomal protein Rpl3p in ribosome synthesis
Valle Rosado, Iván
title_short Functional analysis of saccharomyces cerevisiae ribosomal protein Rpl3p in ribosome synthesis
title_full Functional analysis of saccharomyces cerevisiae ribosomal protein Rpl3p in ribosome synthesis
title_fullStr Functional analysis of saccharomyces cerevisiae ribosomal protein Rpl3p in ribosome synthesis
title_full_unstemmed Functional analysis of saccharomyces cerevisiae ribosomal protein Rpl3p in ribosome synthesis
title_sort Functional analysis of saccharomyces cerevisiae ribosomal protein Rpl3p in ribosome synthesis
dc.creator.none.fl_str_mv Valle Rosado, Iván
Kressler, Dieter
Cruz Díaz, Jesús de la
author Valle Rosado, Iván
author_facet Valle Rosado, Iván
Kressler, Dieter
Cruz Díaz, Jesús de la
author_role author
author2 Kressler, Dieter
Cruz Díaz, Jesús de la
author2_role author
author
dc.contributor.none.fl_str_mv Genética
description Ribosome synthesis in eukaryotes requires a multitude oftrans-acting factors. These factors act at many steps as the pre-ribosomal particles travel from the nucleolus to the cytoplasm. In contrast to the well-studiedtrans-acting factors, little is known about the contribution of the ribosomal proteins to ribosome biogenesis. Herein, we have analysed the role of ribosomal protein Rpl3p in 60S ribosomal subunit biogenesis. In vivo depletion of Rpl3p results in a deficit in 60S ribosomal subunits and the appearance of half-mer polysomes. This phenotype is likely due to the instability of early and intermediate pre-ribosomal particles, as evidenced by the low steady-state levels of 27SA3, 27SBSand 7SL/S precursors. Furthermore, depletion of Rpl3p impairs the nucleocytoplasmic export of pre-60S ribosomal particles. Interestingly, flow cytometry analysis indicates that Rpl3p-depleted cells arrest in the G1 phase. Altogether, we suggest that upon depletion of Rpl3p, early assembly of 60S ribosomal subunits is aborted and subsequent steps during their maturation and export prevented
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/44309
https://doi.org/10.1093/nar/gkm388
url http://hdl.handle.net/11441/44309
https://doi.org/10.1093/nar/gkm388
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Nucleic Acids Research, 35 (12), 4203-4213.
10.1093/nar/gkm388
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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