Monitoring Human Viral Pathogens Reveals Potential Hazard for Treated Wastewater Discharge or Reuse
Wastewater discharge to the environment or its reuse after sanitization poses a concern for public health given the risk of transmission of human viral diseases. However, estimating the viral infectivity along the wastewater cycle presents technical challenges and still remains underexplored. Recent...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/267394 |
| Acceso en línea: | http://hdl.handle.net/10261/267394 |
| Access Level: | acceso abierto |
| Palabra clave: | Enteric viruses Capsid integrity RT-qPCR Wastewater crAssphage Fecal contamination indicator |
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Monitoring Human Viral Pathogens Reveals Potential Hazard for Treated Wastewater Discharge or ReuseCuevas Ferrando, EnricPérez-Cataluña, AlbaFalcó, IreneRandazzo, WalterSánchez Moragas, GloriaEnteric virusesCapsid integrityRT-qPCRWastewatercrAssphageFecal contamination indicatorWastewater discharge to the environment or its reuse after sanitization poses a concern for public health given the risk of transmission of human viral diseases. However, estimating the viral infectivity along the wastewater cycle presents technical challenges and still remains underexplored. Recently, human-associated crAssphage has been investigated to serve as viral pathogen indicator to monitor fecal impacted water bodies, even though its assessment as biomarker for infectious enteric viruses has not been explored yet. To this end, the occurrence of potentially infectious norovirus genogroup I (GI), norovirus GII, hepatitis A virus (HAV), rotavirus A (RV), and human astrovirus (HAstV) along with crAssphage was investigated in influent and effluent water sampled in four wastewater treatment plants (WWTPs) over 1 year by a PMAxx-based capsid integrity RT-qPCR assay. Moreover, influent and effluent samples of a selected WWTP were additionally assayed by an in situ capture RT-qPCR assay (ISC-RT-qPCR) as estimate for viral infectivity in alternative to PMAxx-RT-qPCR. Overall, our results showed lower viral occurrence and concentration assessed by ISC-RT-qPCR than PMAxx-RT-qPCR. Occurrence of potentially infectious enteric virus was estimated by PMAxx-RT-qPCR as 88–94% in influent and 46–67% in effluent wastewaters with mean titers ranging from 4.77 to 5.89, and from 3.86 to 4.97 log10 GC/L, with the exception of HAV that was sporadically detected. All samples tested positive for crAssphage at concentration ranging from 7.41 to 9.99 log10 GC/L in influent and from 4.56 to 6.96 log10 GC/L in effluent wastewater, showing higher mean concentration than targeted enteric viruses. Data obtained by PMAxx-RT-qPCR showed that crAssphage strongly correlated with norovirus GII (ρ = 0.67, p < 0.05) and weakly with HAstV and RV (ρ = 0.25–0.30, p < 0.05) in influent samples. In effluent wastewater, weak (ρ = 0.27–0.38, p < 0.05) to moderate (ρ = 0.47–0.48, p < 0.05) correlations between crAssphage and targeted viruses were observed. Overall, these results corroborate crAssphage as an indicator for fecal contamination in wastewater but a poor marker for either viral occurrence and viral integrity/infectivity. Despite the viral load reductions detected in effluent compared to influent wastewaters, the estimates of viral infectivity based on viability molecular methods might pose a concern for (re)-using of treated water.This research was supported by the “VIRIDIANA” (AGL2017-82909, AEI/FEDER, UE) and “PREVISION” (PID2019-105509RJ-I00, AEI/FEDER, UE) projects funded by the Spanish Ministry of Science, Innovation and Universities, and the 2018CPS10 project funded by the Center for Produce Safety. AP-C was recipient of a postdoctoral grant from Generalitat Valenciana (APOSTD/2021/292). EC-F was recipient of a predoctoral contract from the MICINN, Call 2018.Peer reviewedFrontiers MediaMinisterio de Ciencia e Innovación (España)Center for Produce Safety (US)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/267394reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-82909-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105509RJ-I00https://doi.org/10.3389/fmicb.2022.836193Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2673942026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Monitoring Human Viral Pathogens Reveals Potential Hazard for Treated Wastewater Discharge or Reuse |
| title |
Monitoring Human Viral Pathogens Reveals Potential Hazard for Treated Wastewater Discharge or Reuse |
| spellingShingle |
Monitoring Human Viral Pathogens Reveals Potential Hazard for Treated Wastewater Discharge or Reuse Cuevas Ferrando, Enric Enteric viruses Capsid integrity RT-qPCR Wastewater crAssphage Fecal contamination indicator |
| title_short |
Monitoring Human Viral Pathogens Reveals Potential Hazard for Treated Wastewater Discharge or Reuse |
| title_full |
Monitoring Human Viral Pathogens Reveals Potential Hazard for Treated Wastewater Discharge or Reuse |
| title_fullStr |
Monitoring Human Viral Pathogens Reveals Potential Hazard for Treated Wastewater Discharge or Reuse |
| title_full_unstemmed |
Monitoring Human Viral Pathogens Reveals Potential Hazard for Treated Wastewater Discharge or Reuse |
| title_sort |
Monitoring Human Viral Pathogens Reveals Potential Hazard for Treated Wastewater Discharge or Reuse |
| dc.creator.none.fl_str_mv |
Cuevas Ferrando, Enric Pérez-Cataluña, Alba Falcó, Irene Randazzo, Walter Sánchez Moragas, Gloria |
| author |
Cuevas Ferrando, Enric |
| author_facet |
Cuevas Ferrando, Enric Pérez-Cataluña, Alba Falcó, Irene Randazzo, Walter Sánchez Moragas, Gloria |
| author_role |
author |
| author2 |
Pérez-Cataluña, Alba Falcó, Irene Randazzo, Walter Sánchez Moragas, Gloria |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Ministerio de Ciencia e Innovación (España) Center for Produce Safety (US) Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Enteric viruses Capsid integrity RT-qPCR Wastewater crAssphage Fecal contamination indicator |
| topic |
Enteric viruses Capsid integrity RT-qPCR Wastewater crAssphage Fecal contamination indicator |
| description |
Wastewater discharge to the environment or its reuse after sanitization poses a concern for public health given the risk of transmission of human viral diseases. However, estimating the viral infectivity along the wastewater cycle presents technical challenges and still remains underexplored. Recently, human-associated crAssphage has been investigated to serve as viral pathogen indicator to monitor fecal impacted water bodies, even though its assessment as biomarker for infectious enteric viruses has not been explored yet. To this end, the occurrence of potentially infectious norovirus genogroup I (GI), norovirus GII, hepatitis A virus (HAV), rotavirus A (RV), and human astrovirus (HAstV) along with crAssphage was investigated in influent and effluent water sampled in four wastewater treatment plants (WWTPs) over 1 year by a PMAxx-based capsid integrity RT-qPCR assay. Moreover, influent and effluent samples of a selected WWTP were additionally assayed by an in situ capture RT-qPCR assay (ISC-RT-qPCR) as estimate for viral infectivity in alternative to PMAxx-RT-qPCR. Overall, our results showed lower viral occurrence and concentration assessed by ISC-RT-qPCR than PMAxx-RT-qPCR. Occurrence of potentially infectious enteric virus was estimated by PMAxx-RT-qPCR as 88–94% in influent and 46–67% in effluent wastewaters with mean titers ranging from 4.77 to 5.89, and from 3.86 to 4.97 log10 GC/L, with the exception of HAV that was sporadically detected. All samples tested positive for crAssphage at concentration ranging from 7.41 to 9.99 log10 GC/L in influent and from 4.56 to 6.96 log10 GC/L in effluent wastewater, showing higher mean concentration than targeted enteric viruses. Data obtained by PMAxx-RT-qPCR showed that crAssphage strongly correlated with norovirus GII (ρ = 0.67, p < 0.05) and weakly with HAstV and RV (ρ = 0.25–0.30, p < 0.05) in influent samples. In effluent wastewater, weak (ρ = 0.27–0.38, p < 0.05) to moderate (ρ = 0.47–0.48, p < 0.05) correlations between crAssphage and targeted viruses were observed. Overall, these results corroborate crAssphage as an indicator for fecal contamination in wastewater but a poor marker for either viral occurrence and viral integrity/infectivity. Despite the viral load reductions detected in effluent compared to influent wastewaters, the estimates of viral infectivity based on viability molecular methods might pose a concern for (re)-using of treated water. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2022 2022 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10261/267394 |
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Inglés |
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Inglés |
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#PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-82909-R info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105509RJ-I00 https://doi.org/10.3389/fmicb.2022.836193 Sí |
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