Vascular smooth muscle cell growth arrest on blockade of thrombospondin-1 requires p21Cip1/WAF1

7 páginas, 4 figuras.-- El documento en word es la versión post-print

Detalles Bibliográficos
Autores: Chen, Donghui, Guo, Kun, Yang, Jihong, Frazier, William A., Isner, Jeffrey M., Andrés, Vicente
Tipo de recurso: artículo
Fecha de publicación:1999
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/36429
Acceso en línea:http://hdl.handle.net/10261/36429
Access Level:acceso abierto
Palabra clave:Cell cycle control
p21Cip1/WAF1
Thrombospondin
Extracellular matrix
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spelling Vascular smooth muscle cell growth arrest on blockade of thrombospondin-1 requires p21Cip1/WAF1Chen, DonghuiGuo, KunYang, JihongFrazier, William A.Isner, Jeffrey M.Andrés, VicenteCell cycle controlp21Cip1/WAF1ThrombospondinExtracellular matrix7 páginas, 4 figuras.-- El documento en word es la versión post-printAbnormal proliferation of vascular smooth muscle cells (VSMCs) is thought to play an important role in the pathogenesis of atherosclerosis and restenosis. Previous studies have implicated the extracellular matrix protein thrombospondin-1 (TSP1) in mitogen-dependent proliferation of VSMCs. In this study, we investigated the molecular mechanisms involved in TSP1-mediated regulation of VSMC growth. Neutralizing A4.1 anti-TSP1 antibody inhibited the activity of the G1/S cyclin-dependent kinase 2 (cdk2) and blocked the induction of S-phase entry, which normally occurs in serum-stimulated VSMCs. This growth-inhibitory effect was associated with a marked induction of p21Cip1/WAF1 (p21) expression in A4.1-treated VSMCs. Moreover, addition of A4.1 antibody to VSMCs markedly increased the level of p21 bound to cdk2. Thus growth arrest on antibody blockade of TSP1 may be mediated by the cdk inhibitory protein p21. Consistent with this notion, anti-TSP1 antibody inhibited [3H]-thymidine incorporation in wild-type but not in p21-deficient mouse embryonic fibroblasts (MEFs). Together, these data suggest that p21 plays an important role in TSP1-mediated control of cellular proliferation.This paper was supported in part by National Institutes of Health Grants HL-57519 and AG-15227 (to V. Andrés); HL-40518, HL-53354, and HL-57516 (to J. M. Isner); and CA-65872 (toW. A. Frazier).Peer reviewedAmerican Physiological Society201120111999info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/36429reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://ajpheart.physiology.org/content/277/3/H1100.abstractinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/364292026-05-22T06:33:51Z
dc.title.none.fl_str_mv Vascular smooth muscle cell growth arrest on blockade of thrombospondin-1 requires p21Cip1/WAF1
title Vascular smooth muscle cell growth arrest on blockade of thrombospondin-1 requires p21Cip1/WAF1
spellingShingle Vascular smooth muscle cell growth arrest on blockade of thrombospondin-1 requires p21Cip1/WAF1
Chen, Donghui
Cell cycle control
p21Cip1/WAF1
Thrombospondin
Extracellular matrix
title_short Vascular smooth muscle cell growth arrest on blockade of thrombospondin-1 requires p21Cip1/WAF1
title_full Vascular smooth muscle cell growth arrest on blockade of thrombospondin-1 requires p21Cip1/WAF1
title_fullStr Vascular smooth muscle cell growth arrest on blockade of thrombospondin-1 requires p21Cip1/WAF1
title_full_unstemmed Vascular smooth muscle cell growth arrest on blockade of thrombospondin-1 requires p21Cip1/WAF1
title_sort Vascular smooth muscle cell growth arrest on blockade of thrombospondin-1 requires p21Cip1/WAF1
dc.creator.none.fl_str_mv Chen, Donghui
Guo, Kun
Yang, Jihong
Frazier, William A.
Isner, Jeffrey M.
Andrés, Vicente
author Chen, Donghui
author_facet Chen, Donghui
Guo, Kun
Yang, Jihong
Frazier, William A.
Isner, Jeffrey M.
Andrés, Vicente
author_role author
author2 Guo, Kun
Yang, Jihong
Frazier, William A.
Isner, Jeffrey M.
Andrés, Vicente
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cell cycle control
p21Cip1/WAF1
Thrombospondin
Extracellular matrix
topic Cell cycle control
p21Cip1/WAF1
Thrombospondin
Extracellular matrix
description 7 páginas, 4 figuras.-- El documento en word es la versión post-print
publishDate 1999
dc.date.none.fl_str_mv 1999
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/36429
url http://hdl.handle.net/10261/36429
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://ajpheart.physiology.org/content/277/3/H1100.abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Physiological Society
publisher.none.fl_str_mv American Physiological Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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