First‐Pass Metabolism of Chlorophylls in Mice

33 Páginas; 2 Tablas; 2 Figuras

Detalles Bibliográficos
Autores: Viera Alcaide, Isabel, Chen, Kewei, Ríos, José Julián, Benito, Itziar, Pérez Gálvez, Antonio, Roca, María
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/171165
Acceso en línea:http://hdl.handle.net/10261/171165
Access Level:acceso abierto
Palabra clave:Absorption
Chlorophylls
Liver
Pheophorbide
SR-BI
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repository_id_str
spelling First‐Pass Metabolism of Chlorophylls in MiceViera Alcaide, IsabelChen, KeweiRíos, José JuliánBenito, ItziarPérez Gálvez, AntonioRoca, MaríaAbsorptionChlorophyllsLiverPheophorbideSR-BI33 Páginas; 2 Tablas; 2 Figuras1 Scope The dietary intake of chlorophylls is estimated to be ≈50 mg d–1. However, their first pass metabolism and systemic assimilation is not well characterized. 2 Methods and results A group of 30 mice are fed a diet rich in chlorophylls, while 10 mice received a standard diet without chlorophylls (control group). Liver extracts are analyzed every 15 days by HPLC–ESI(+)/APCI(+)‐hrTOF– MS/MS to measure the accretion of specific chlorophyll metabolites. The chlorophyll profile found in the livers of mice fed a chlorophyll‐rich diet shows that the formation and/or absorption of pheophorbides, pyro‐derivatives, and phytyl‐chlorin e6 require the occurrence of a precise first‐pass metabolism. In addition, the apical absorption of pheorphorbide a‐rich micelles is significantly inhibited in Caucasian colon adenocarcinoma‐2 cells pre‐incubated with BLT1. 3 Conclusion Pheophorbide a absorption is, at least partly, protein‐mediated through SR‐BI. This active absorption process could explain the specific accumulation of pheophorbide a in the livers of animals fed a chlorophyll‐rich diet. A complementary mechanism could be the de‐esterification of pheophytin a in the liver, yielding pheophorbide a and phytol, which can explain the origin of phytol in the liver. Hence, the results suggest two molecular mechanisms responsible for the accumulation of the health‐promoting compounds pheophorbide and phytol.This work was supported by the Comisión Interministerial de Ciencia y Tecnología (CICYT‐EU, Spanish and European Government, grant number AGL 2015‐63890‐R).Peer reviewedJohn Wiley & SonsComisión Interministerial de Ciencia y Tecnología, CICYT (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/171165reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL 2015‐63890‐Rhttp://dx.doi.org/10.1002/mnfr.201800562Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1711652026-05-22T06:33:51Z
dc.title.none.fl_str_mv First‐Pass Metabolism of Chlorophylls in Mice
title First‐Pass Metabolism of Chlorophylls in Mice
spellingShingle First‐Pass Metabolism of Chlorophylls in Mice
Viera Alcaide, Isabel
Absorption
Chlorophylls
Liver
Pheophorbide
SR-BI
title_short First‐Pass Metabolism of Chlorophylls in Mice
title_full First‐Pass Metabolism of Chlorophylls in Mice
title_fullStr First‐Pass Metabolism of Chlorophylls in Mice
title_full_unstemmed First‐Pass Metabolism of Chlorophylls in Mice
title_sort First‐Pass Metabolism of Chlorophylls in Mice
dc.creator.none.fl_str_mv Viera Alcaide, Isabel
Chen, Kewei
Ríos, José Julián
Benito, Itziar
Pérez Gálvez, Antonio
Roca, María
author Viera Alcaide, Isabel
author_facet Viera Alcaide, Isabel
Chen, Kewei
Ríos, José Julián
Benito, Itziar
Pérez Gálvez, Antonio
Roca, María
author_role author
author2 Chen, Kewei
Ríos, José Julián
Benito, Itziar
Pérez Gálvez, Antonio
Roca, María
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Absorption
Chlorophylls
Liver
Pheophorbide
SR-BI
topic Absorption
Chlorophylls
Liver
Pheophorbide
SR-BI
description 33 Páginas; 2 Tablas; 2 Figuras
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/171165
url http://hdl.handle.net/10261/171165
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL 2015‐63890‐R
http://dx.doi.org/10.1002/mnfr.201800562
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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