Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach.

The total damage inflicted on the liver before transplantation is associated with severalsurgical manipulations, such as organ recovery, washout of the graft, cold conservation in organpreservation solutions (UW, Celsior, HTK, IGL-1), and rinsing of the organ before implantation.Polyethylene glycol...

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Authors: Bardallo, Raquel G., Da Silva, Rui Teixeira, Carbonell i Camós, Teresa, Folch i Puy, Emma, Palmeira, Carlos M., Roselló Catafau, Juan, Pirenne, Jacques, Adam, R. (René), Panisello Roselló, Arnau
Format: article
Status:Published version
Publication Date:2021
Country:España
Institution:Universidad de Barcelona
Repository:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/180436
Online Access:https://hdl.handle.net/2445/180436
Access Level:Open access
Keyword:Malalties del fetge
Òxid nítric
Trasplantament hepàtic
Liver diseases
Nitric oxide
Hepatic transplantation
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repository_id_str
spelling Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach.Bardallo, Raquel G.Da Silva, Rui TeixeiraCarbonell i Camós, TeresaFolch i Puy, EmmaPalmeira, Carlos M.Roselló Catafau, JuanPirenne, JacquesAdam, R. (René)Panisello Roselló, ArnauMalalties del fetgeÒxid nítricTrasplantament hepàticLiver diseasesNitric oxideHepatic transplantationThe total damage inflicted on the liver before transplantation is associated with severalsurgical manipulations, such as organ recovery, washout of the graft, cold conservation in organpreservation solutions (UW, Celsior, HTK, IGL-1), and rinsing of the organ before implantation.Polyethylene glycol 35 (PEG35) is the oncotic agent present in the IGL-1 solution, which is an alterna-tive to UW and Celsior solutions in liver clinical transplantation. In a model of cold preservation inrats (4◦C; 24 h), we evaluated the effects induced by PEG35 on detoxifying enzymes and nitric oxide,comparing IGL-1 to IGL-0 (which is the same as IGL-1 without PEG). The benefits were also assessedin a new IGL-2 solution characterized by increased concentrations of PEG35 (from 1 g/L to 5 g/L)and glutathione (from 3 mmol/L to 9 mmol/L) compared to IGL-1. We demonstrated that PEG35promoted the mitochondrial enzyme ALDH2, and in combination with glutathione, prevented theformation of toxic aldehyde adducts (measured as 4-hydroxynonenal) and oxidized proteins (AOPP).In addition, PEG35 promoted the vasodilator factor nitric oxide, which may improve the microcircu-latory disturbances in steatotic grafts during preservation and revascularization. All of these resultslead to a reduction in damage inflicted on the fatty liver graft during the cold storage preservation.In this communication, we report on the benefits of IGL-2 in hypothermic static preservation, whichhas already been proved to confer benefits in hypothermic oxygenated dynamic preservation. Hence,the data reported here reinforce the fact that IGL-2 is a suitable alternative to be used as a uniquesolution/perfusate when hypothermic static and preservation strategies are used, either separately orcombined, easing the logistics and avoiding the mixture of different solutions/perfusates, especiallywhen fatty liver grafts are used. Further research regarding new therapeutic and pharmacologicalinsights is needed to explore the underlying mitochondrial mechanisms exerted by PEG35 in staticand dynamic graft preservation strategies for clinical liver transplantation purposeMDPI2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/180436Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/ijms22105332International Journal of Molecular Sciences, 2021, vol. 22, num. 10, p. 1-13https://doi.org/10.3390/ijms22105332info:eu-repo/grantAgreement/EC/H2020/722619cc-by (c) Bardallo, Raquel G. et al., 2021https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1804362026-05-27T06:46:51Z
dc.title.none.fl_str_mv Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach.
title Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach.
spellingShingle Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach.
Bardallo, Raquel G.
Malalties del fetge
Òxid nítric
Trasplantament hepàtic
Liver diseases
Nitric oxide
Hepatic transplantation
title_short Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach.
title_full Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach.
title_fullStr Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach.
title_full_unstemmed Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach.
title_sort Role of PEG35, Mitochondrial ALDH2, and Glutathione in Cold Fatty Liver Graft Preservation: An IGL-2 Approach.
dc.creator.none.fl_str_mv Bardallo, Raquel G.
Da Silva, Rui Teixeira
Carbonell i Camós, Teresa
Folch i Puy, Emma
Palmeira, Carlos M.
Roselló Catafau, Juan
Pirenne, Jacques
Adam, R. (René)
Panisello Roselló, Arnau
author Bardallo, Raquel G.
author_facet Bardallo, Raquel G.
Da Silva, Rui Teixeira
Carbonell i Camós, Teresa
Folch i Puy, Emma
Palmeira, Carlos M.
Roselló Catafau, Juan
Pirenne, Jacques
Adam, R. (René)
Panisello Roselló, Arnau
author_role author
author2 Da Silva, Rui Teixeira
Carbonell i Camós, Teresa
Folch i Puy, Emma
Palmeira, Carlos M.
Roselló Catafau, Juan
Pirenne, Jacques
Adam, R. (René)
Panisello Roselló, Arnau
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Malalties del fetge
Òxid nítric
Trasplantament hepàtic
Liver diseases
Nitric oxide
Hepatic transplantation
topic Malalties del fetge
Òxid nítric
Trasplantament hepàtic
Liver diseases
Nitric oxide
Hepatic transplantation
description The total damage inflicted on the liver before transplantation is associated with severalsurgical manipulations, such as organ recovery, washout of the graft, cold conservation in organpreservation solutions (UW, Celsior, HTK, IGL-1), and rinsing of the organ before implantation.Polyethylene glycol 35 (PEG35) is the oncotic agent present in the IGL-1 solution, which is an alterna-tive to UW and Celsior solutions in liver clinical transplantation. In a model of cold preservation inrats (4◦C; 24 h), we evaluated the effects induced by PEG35 on detoxifying enzymes and nitric oxide,comparing IGL-1 to IGL-0 (which is the same as IGL-1 without PEG). The benefits were also assessedin a new IGL-2 solution characterized by increased concentrations of PEG35 (from 1 g/L to 5 g/L)and glutathione (from 3 mmol/L to 9 mmol/L) compared to IGL-1. We demonstrated that PEG35promoted the mitochondrial enzyme ALDH2, and in combination with glutathione, prevented theformation of toxic aldehyde adducts (measured as 4-hydroxynonenal) and oxidized proteins (AOPP).In addition, PEG35 promoted the vasodilator factor nitric oxide, which may improve the microcircu-latory disturbances in steatotic grafts during preservation and revascularization. All of these resultslead to a reduction in damage inflicted on the fatty liver graft during the cold storage preservation.In this communication, we report on the benefits of IGL-2 in hypothermic static preservation, whichhas already been proved to confer benefits in hypothermic oxygenated dynamic preservation. Hence,the data reported here reinforce the fact that IGL-2 is a suitable alternative to be used as a uniquesolution/perfusate when hypothermic static and preservation strategies are used, either separately orcombined, easing the logistics and avoiding the mixture of different solutions/perfusates, especiallywhen fatty liver grafts are used. Further research regarding new therapeutic and pharmacologicalinsights is needed to explore the underlying mitochondrial mechanisms exerted by PEG35 in staticand dynamic graft preservation strategies for clinical liver transplantation purpose
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/180436
url https://hdl.handle.net/2445/180436
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/ijms22105332
International Journal of Molecular Sciences, 2021, vol. 22, num. 10, p. 1-13
https://doi.org/10.3390/ijms22105332
info:eu-repo/grantAgreement/EC/H2020/722619
dc.rights.none.fl_str_mv cc-by (c) Bardallo, Raquel G. et al., 2021
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Bardallo, Raquel G. et al., 2021
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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