Determination of ligand profiles for pseudomonas aeruginosa solute binding proteins

Solute binding proteins (SBPs) form a heterogeneous protein family that is found in all kingdoms of life. In bacteria, the ligand-loaded forms bind to transmembrane transporters providing the substrate. We present here the SBP repertoire of Pseudomonas aeruginosa PAO1 that is composed of 98 proteins...

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Detalles Bibliográficos
Autores: Fernández, Matilde, Rico-Jiménez, Miriam, Ortega, Álvaro, Daddaoua, Abdelali, García-García, Ana L., Martín-Mora, David, Mesa Torres, Noel, Tajuelo, Ana, Matilla, Miguel A., Krell, Tino
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/193520
Acceso en línea:http://hdl.handle.net/10261/193520
Access Level:acceso abierto
Palabra clave:Solute binding protein
Transport
Chemotaxis
Ligand recognition
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spelling Determination of ligand profiles for pseudomonas aeruginosa solute binding proteinsFernández, MatildeRico-Jiménez, MiriamOrtega, ÁlvaroDaddaoua, AbdelaliGarcía-García, Ana L.Martín-Mora, DavidMesa Torres, NoelTajuelo, AnaMatilla, Miguel A.Krell, TinoSolute binding proteinTransportChemotaxisLigand recognitionSolute binding proteins (SBPs) form a heterogeneous protein family that is found in all kingdoms of life. In bacteria, the ligand-loaded forms bind to transmembrane transporters providing the substrate. We present here the SBP repertoire of Pseudomonas aeruginosa PAO1 that is composed of 98 proteins. Bioinformatic predictions indicate that many of these proteins have a redundant ligand profile such as 27 SBPs for proteinogenic amino acids, 13 proteins for spermidine/putrescine, or 9 proteins for quaternary amines. To assess the precision of these bioinformatic predictions, we have purified 17 SBPs that were subsequently submitted to high-throughput ligand screening approaches followed by isothermal titration calorimetry studies, resulting in the identification of ligands for 15 of them. Experimentation revealed that PA0222 was specific for γ-aminobutyrate (GABA), DppA2 for tripeptides, DppA3 for dipeptides, CysP for thiosulphate, OpuCC for betaine, and AotJ for arginine. Furthermore, RbsB bound D-ribose and D-allose, ModA bound molybdate, tungstate, and chromate, whereas AatJ recognized aspartate and glutamate. The majority of experimentally identified ligands were found to be chemoattractants. Data show that the ligand class recognized by SPBs can be predicted with confidence using bioinformatic methods, but experimental work is necessary to identify the precise ligand profile.This work was supported by FEDER funds and Fondo Social Europeo through a grant from the Spanish Ministry for Economy and Competitiveness to T. Krell (BIO2016-76779-P).Peer reviewedMultidisciplinary Digital Publishing InstituteEuropean CommissionMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/193520reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-76779-Phttps://doi.org/10.3390/ijms20205156Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1935202026-05-22T06:33:51Z
dc.title.none.fl_str_mv Determination of ligand profiles for pseudomonas aeruginosa solute binding proteins
title Determination of ligand profiles for pseudomonas aeruginosa solute binding proteins
spellingShingle Determination of ligand profiles for pseudomonas aeruginosa solute binding proteins
Fernández, Matilde
Solute binding protein
Transport
Chemotaxis
Ligand recognition
title_short Determination of ligand profiles for pseudomonas aeruginosa solute binding proteins
title_full Determination of ligand profiles for pseudomonas aeruginosa solute binding proteins
title_fullStr Determination of ligand profiles for pseudomonas aeruginosa solute binding proteins
title_full_unstemmed Determination of ligand profiles for pseudomonas aeruginosa solute binding proteins
title_sort Determination of ligand profiles for pseudomonas aeruginosa solute binding proteins
dc.creator.none.fl_str_mv Fernández, Matilde
Rico-Jiménez, Miriam
Ortega, Álvaro
Daddaoua, Abdelali
García-García, Ana L.
Martín-Mora, David
Mesa Torres, Noel
Tajuelo, Ana
Matilla, Miguel A.
Krell, Tino
author Fernández, Matilde
author_facet Fernández, Matilde
Rico-Jiménez, Miriam
Ortega, Álvaro
Daddaoua, Abdelali
García-García, Ana L.
Martín-Mora, David
Mesa Torres, Noel
Tajuelo, Ana
Matilla, Miguel A.
Krell, Tino
author_role author
author2 Rico-Jiménez, Miriam
Ortega, Álvaro
Daddaoua, Abdelali
García-García, Ana L.
Martín-Mora, David
Mesa Torres, Noel
Tajuelo, Ana
Matilla, Miguel A.
Krell, Tino
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Solute binding protein
Transport
Chemotaxis
Ligand recognition
topic Solute binding protein
Transport
Chemotaxis
Ligand recognition
description Solute binding proteins (SBPs) form a heterogeneous protein family that is found in all kingdoms of life. In bacteria, the ligand-loaded forms bind to transmembrane transporters providing the substrate. We present here the SBP repertoire of Pseudomonas aeruginosa PAO1 that is composed of 98 proteins. Bioinformatic predictions indicate that many of these proteins have a redundant ligand profile such as 27 SBPs for proteinogenic amino acids, 13 proteins for spermidine/putrescine, or 9 proteins for quaternary amines. To assess the precision of these bioinformatic predictions, we have purified 17 SBPs that were subsequently submitted to high-throughput ligand screening approaches followed by isothermal titration calorimetry studies, resulting in the identification of ligands for 15 of them. Experimentation revealed that PA0222 was specific for γ-aminobutyrate (GABA), DppA2 for tripeptides, DppA3 for dipeptides, CysP for thiosulphate, OpuCC for betaine, and AotJ for arginine. Furthermore, RbsB bound D-ribose and D-allose, ModA bound molybdate, tungstate, and chromate, whereas AatJ recognized aspartate and glutamate. The majority of experimentally identified ligands were found to be chemoattractants. Data show that the ligand class recognized by SPBs can be predicted with confidence using bioinformatic methods, but experimental work is necessary to identify the precise ligand profile.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/193520
url http://hdl.handle.net/10261/193520
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-76779-P
https://doi.org/10.3390/ijms20205156
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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