Age-associated changes in intestinal health biomarkers in dogs

The gut microbiome is critical for maintaining host health. In healthy humans, the aging process is one of the main factors modulating the changes in the intestinal microbiota. However, little is known about the relationship between gut health, microbiota, and the aging process in dogs. The present...

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Autores: Fernández Pinteño, Anna, Pilla, Rachel, Manteca, Xavier, Suchodolski, Jan, Torre, Celina, Salas Mani, Anna
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/202537
Acceso en línea:https://hdl.handle.net/2445/202537
Access Level:acceso abierto
Palabra clave:Microbiota intestinal
Gossos
Marcadors bioquímics
Gastrointestinal microbiome
Dogs
Biochemical markers
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spelling Age-associated changes in intestinal health biomarkers in dogsFernández Pinteño, AnnaPilla, RachelManteca, XavierSuchodolski, JanTorre, CelinaSalas Mani, AnnaMicrobiota intestinalGossosMarcadors bioquímicsGastrointestinal microbiomeDogsBiochemical markersThe gut microbiome is critical for maintaining host health. In healthy humans, the aging process is one of the main factors modulating the changes in the intestinal microbiota. However, little is known about the relationship between gut health, microbiota, and the aging process in dogs. The present study aims to explore the differences in the intestinal microbiota and intestinal health based on fecal biomarkers in a population of dogs of different ages. The study involved 106 dogs of different breeds aged between 0.2 and 15 years categorized as senior (>7 years; n = 40), adult (2-7 years; n = 50), and junior (< 2 years; n = 16). Fecal samples were collected during the same period at the same facilities. The analysis included the following gut health indicators: 16S rRNA gene sequencing to investigate the differences in the fecal microbiota; qPCR to determine the dysbiosis index; fecal short-chain fatty acid concentrations; fecal calprotectin; and immunoglobulin A. Beta diversity analysis revealed a significant difference with a small effect size (p = 0.003; R = 0.087) among age categories based on the unweighted UniFrac metric, but no significance was observed based on the weighted UniFrac metric or Bray-Curtis distances. There were no significant differences in the alpha diversity measures or the fecal dysbiosis index among age categories. Senior dogs had significantly higher relative abundance proportions in phyla Bacteroidota and Pseudomonadota and the genus Faecalibacterium, but not on qPCR analysis. At the family level, Ruminococcaceae, Uncl. Clostridiales.1, Veillonellaceae, Prevotellaceae, Succinivibrionaceae, and Bacteroidaceae abundances were higher in the senior category than in the adult and/or junior categories. Relative proportions, but not concentrations of fecal acetate, were higher in the senior category, while butyrate, isovaleric acid, and valeric acid were lower. The valeric acid concentration was significantly lower in the senior category than in the adult category. Calprotectin and immunoglobulin A levels did not differ significantly across groups. In conclusion, this study observed multiple minor changes in the fecal microbiota composition and the relative amount of short-chain fatty acids in dogs among different age groups, but studies in larger populations representative of all ages are warranted to refine the present results.Frontiers Media SA2023202320232023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion13 p.application/pdfhttps://hdl.handle.net/2445/202537Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3389/fvets.2023.1213287Frontiers in Veterinary Science, 2023, vol. 10https://doi.org/10.3389/fvets.2023.1213287cc by (c) Fernández Pinteño, Anna et al., 2023http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2025372026-05-29T05:05:01Z
dc.title.none.fl_str_mv Age-associated changes in intestinal health biomarkers in dogs
title Age-associated changes in intestinal health biomarkers in dogs
spellingShingle Age-associated changes in intestinal health biomarkers in dogs
Fernández Pinteño, Anna
Microbiota intestinal
Gossos
Marcadors bioquímics
Gastrointestinal microbiome
Dogs
Biochemical markers
title_short Age-associated changes in intestinal health biomarkers in dogs
title_full Age-associated changes in intestinal health biomarkers in dogs
title_fullStr Age-associated changes in intestinal health biomarkers in dogs
title_full_unstemmed Age-associated changes in intestinal health biomarkers in dogs
title_sort Age-associated changes in intestinal health biomarkers in dogs
dc.creator.none.fl_str_mv Fernández Pinteño, Anna
Pilla, Rachel
Manteca, Xavier
Suchodolski, Jan
Torre, Celina
Salas Mani, Anna
author Fernández Pinteño, Anna
author_facet Fernández Pinteño, Anna
Pilla, Rachel
Manteca, Xavier
Suchodolski, Jan
Torre, Celina
Salas Mani, Anna
author_role author
author2 Pilla, Rachel
Manteca, Xavier
Suchodolski, Jan
Torre, Celina
Salas Mani, Anna
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Microbiota intestinal
Gossos
Marcadors bioquímics
Gastrointestinal microbiome
Dogs
Biochemical markers
topic Microbiota intestinal
Gossos
Marcadors bioquímics
Gastrointestinal microbiome
Dogs
Biochemical markers
description The gut microbiome is critical for maintaining host health. In healthy humans, the aging process is one of the main factors modulating the changes in the intestinal microbiota. However, little is known about the relationship between gut health, microbiota, and the aging process in dogs. The present study aims to explore the differences in the intestinal microbiota and intestinal health based on fecal biomarkers in a population of dogs of different ages. The study involved 106 dogs of different breeds aged between 0.2 and 15 years categorized as senior (>7 years; n = 40), adult (2-7 years; n = 50), and junior (< 2 years; n = 16). Fecal samples were collected during the same period at the same facilities. The analysis included the following gut health indicators: 16S rRNA gene sequencing to investigate the differences in the fecal microbiota; qPCR to determine the dysbiosis index; fecal short-chain fatty acid concentrations; fecal calprotectin; and immunoglobulin A. Beta diversity analysis revealed a significant difference with a small effect size (p = 0.003; R = 0.087) among age categories based on the unweighted UniFrac metric, but no significance was observed based on the weighted UniFrac metric or Bray-Curtis distances. There were no significant differences in the alpha diversity measures or the fecal dysbiosis index among age categories. Senior dogs had significantly higher relative abundance proportions in phyla Bacteroidota and Pseudomonadota and the genus Faecalibacterium, but not on qPCR analysis. At the family level, Ruminococcaceae, Uncl. Clostridiales.1, Veillonellaceae, Prevotellaceae, Succinivibrionaceae, and Bacteroidaceae abundances were higher in the senior category than in the adult and/or junior categories. Relative proportions, but not concentrations of fecal acetate, were higher in the senior category, while butyrate, isovaleric acid, and valeric acid were lower. The valeric acid concentration was significantly lower in the senior category than in the adult category. Calprotectin and immunoglobulin A levels did not differ significantly across groups. In conclusion, this study observed multiple minor changes in the fecal microbiota composition and the relative amount of short-chain fatty acids in dogs among different age groups, but studies in larger populations representative of all ages are warranted to refine the present results.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/202537
url https://hdl.handle.net/2445/202537
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fvets.2023.1213287
Frontiers in Veterinary Science, 2023, vol. 10
https://doi.org/10.3389/fvets.2023.1213287
dc.rights.none.fl_str_mv cc by (c) Fernández Pinteño, Anna et al., 2023
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Fernández Pinteño, Anna et al., 2023
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13 p.
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media SA
publisher.none.fl_str_mv Frontiers Media SA
dc.source.none.fl_str_mv Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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