A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines

The existence of a subpopulation of undifferentiated cells with stem-like properties has been suggested in neuroblastoma tumors, but a definitive biomarker for their successful isolation is missing. Here we describe an in vitro culture system for the enrichment in undifferentiated stem-like tumor ce...

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Detalles Bibliográficos
Autores: Amador-Álvarez, Aida, Gómez-Muñoz, María A., Rodríguez-Prieto, Ismael, Pardal Redondo, Ricardo, Vega, Francisco M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/296096
Acceso en línea:http://hdl.handle.net/10261/296096
https://api.elsevier.com/content/abstract/scopus_id/85126904913
Access Level:acceso abierto
Palabra clave:Stem cells
Cancer
Cell biology
Cell culture
Cell isolation
Flow Cytometry/Mass Cytometry
Single cell
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spelling A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell linesAmador-Álvarez, AidaGómez-Muñoz, María A.Rodríguez-Prieto, IsmaelPardal Redondo, RicardoVega, Francisco M.Stem cellsCancerCell biologyCell cultureCell isolationFlow Cytometry/Mass CytometrySingle cellThe existence of a subpopulation of undifferentiated cells with stem-like properties has been suggested in neuroblastoma tumors, but a definitive biomarker for their successful isolation is missing. Here we describe an in vitro culture system for the enrichment in undifferentiated stem-like tumor cells for subsequent functional assays. We make use of clonal non-adherent cell culture conditions together with cell sorting with specific expression markers. This protocol allows for the differential study of heterogeneous cell population in neuroblastoma tumors. For complete details on the use and execution of this protocol, please refer to Vega et al. (2019).This research is supported by grants PID2019-110817RB-I00 funded by MCIN/AEI/ 10.13039/501100011033, grants P18-RT-3151 and US-1262985 funded by Junta de Andalucía-Universidad de Sevilla, and by “ERDF A way of making Europe”. M.A.G. was partially supported by a fellowship from the “Asociación Niños Enfermos de Neuroblastoma (NEN)”. A.A.A. is supported by a FPU grant from the Ministry of Universities.Peer reviewedElsevierAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Junta de AndalucíaUniversidad de SevillaEuropean CommissionAsociación Niños Enfermos de Neuroblastoma (España)Ministerio de Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/296096https://api.elsevier.com/content/abstract/scopus_id/85126904913reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110817RB-I00https://doi.org/10.1016/j.xpro.2022.101260Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2960962026-05-22T06:33:51Z
dc.title.none.fl_str_mv A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines
title A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines
spellingShingle A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines
Amador-Álvarez, Aida
Stem cells
Cancer
Cell biology
Cell culture
Cell isolation
Flow Cytometry/Mass Cytometry
Single cell
title_short A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines
title_full A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines
title_fullStr A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines
title_full_unstemmed A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines
title_sort A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines
dc.creator.none.fl_str_mv Amador-Álvarez, Aida
Gómez-Muñoz, María A.
Rodríguez-Prieto, Ismael
Pardal Redondo, Ricardo
Vega, Francisco M.
author Amador-Álvarez, Aida
author_facet Amador-Álvarez, Aida
Gómez-Muñoz, María A.
Rodríguez-Prieto, Ismael
Pardal Redondo, Ricardo
Vega, Francisco M.
author_role author
author2 Gómez-Muñoz, María A.
Rodríguez-Prieto, Ismael
Pardal Redondo, Ricardo
Vega, Francisco M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Junta de Andalucía
Universidad de Sevilla
European Commission
Asociación Niños Enfermos de Neuroblastoma (España)
Ministerio de Universidades (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Stem cells
Cancer
Cell biology
Cell culture
Cell isolation
Flow Cytometry/Mass Cytometry
Single cell
topic Stem cells
Cancer
Cell biology
Cell culture
Cell isolation
Flow Cytometry/Mass Cytometry
Single cell
description The existence of a subpopulation of undifferentiated cells with stem-like properties has been suggested in neuroblastoma tumors, but a definitive biomarker for their successful isolation is missing. Here we describe an in vitro culture system for the enrichment in undifferentiated stem-like tumor cells for subsequent functional assays. We make use of clonal non-adherent cell culture conditions together with cell sorting with specific expression markers. This protocol allows for the differential study of heterogeneous cell population in neuroblastoma tumors. For complete details on the use and execution of this protocol, please refer to Vega et al. (2019).
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/296096
https://api.elsevier.com/content/abstract/scopus_id/85126904913
url http://hdl.handle.net/10261/296096
https://api.elsevier.com/content/abstract/scopus_id/85126904913
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110817RB-I00
https://doi.org/10.1016/j.xpro.2022.101260
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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