Cloning of a Cicer arietinum β-Galactosidase with Pectin-Degrading Function

The cDNA clone (CanBGal-3) encoding a cell wall pectin- degrading  -galactosidase ( III-Gal) from Cicer arietinum L. cv. Castellana has been identified. The identification was carried out by comparing the deduced amino acid sequences of several isolated chickpea  -galactosidase clones with the purif...

Descripción completa

Detalles Bibliográficos
Autores: Esteban Gallego, María Rocío, Dopico, Berta, Muñoz, Francisco J., Romo, Silvia, Martín Sánchez, José Ignacio, Labrador, Emilia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2003
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/156957
Acceso en línea:http://hdl.handle.net/10366/156957
Access Level:acceso abierto
Palabra clave:Cicer arietinum
Elongation
Epicotyls
Galactosidases
Growth
id ES_37152c1f049fce612381ddcdf9e4e16a
oai_identifier_str oai:gredos.usal.es:10366/156957
network_acronym_str ES
network_name_str España
repository_id_str
spelling Cloning of a Cicer arietinum β-Galactosidase with Pectin-Degrading FunctionEsteban Gallego, María RocíoDopico, BertaMuñoz, Francisco J.Romo, SilviaMartín Sánchez, José IgnacioLabrador, EmiliaCicer arietinumElongationEpicotylsGalactosidasesGrowthThe cDNA clone (CanBGal-3) encoding a cell wall pectin- degrading  -galactosidase ( III-Gal) from Cicer arietinum L. cv. Castellana has been identified. The identification was carried out by comparing the deduced amino acid sequences of several isolated chickpea  -galactosidase clones with the purified  III-Gal protein sequence. The expression pattern of the gene corresponding to CanBGal-3 was in concordance with the fluctuations of the enzyme  III-Gal in different seedling organs, being specific to elongating organs such as epicotyls and roots. Transformation of Solanum tuberosum plants with the chickpea CanBGal-3 clone indicated that the  -galactosidase encoded by this clone is a pectin-degrading enzyme. The authors propose an important role for chickpea  III-Gal in pectin degradation in cell walls of vegetative organs such as epicotyls and roots. The degradation of galactan carried out by this enzyme may determine structural changes and affect cell wall porosity. It is suggested that the increase in the size of cell wall pores could permit access of other cell wall-modifying enzymes to their substrate.This work was supported by a grant from the EC (CT97 2224) and a grant from the Dirección General de Enseñanza Superior e Investigación Científica, Spain (PB98–0290).202420242003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/156957reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésCT97 2224PB98–0290Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1569572026-06-07T06:28:51Z
dc.title.none.fl_str_mv Cloning of a Cicer arietinum β-Galactosidase with Pectin-Degrading Function
title Cloning of a Cicer arietinum β-Galactosidase with Pectin-Degrading Function
spellingShingle Cloning of a Cicer arietinum β-Galactosidase with Pectin-Degrading Function
Esteban Gallego, María Rocío
Cicer arietinum
Elongation
Epicotyls
Galactosidases
Growth
title_short Cloning of a Cicer arietinum β-Galactosidase with Pectin-Degrading Function
title_full Cloning of a Cicer arietinum β-Galactosidase with Pectin-Degrading Function
title_fullStr Cloning of a Cicer arietinum β-Galactosidase with Pectin-Degrading Function
title_full_unstemmed Cloning of a Cicer arietinum β-Galactosidase with Pectin-Degrading Function
title_sort Cloning of a Cicer arietinum β-Galactosidase with Pectin-Degrading Function
dc.creator.none.fl_str_mv Esteban Gallego, María Rocío
Dopico, Berta
Muñoz, Francisco J.
Romo, Silvia
Martín Sánchez, José Ignacio
Labrador, Emilia
author Esteban Gallego, María Rocío
author_facet Esteban Gallego, María Rocío
Dopico, Berta
Muñoz, Francisco J.
Romo, Silvia
Martín Sánchez, José Ignacio
Labrador, Emilia
author_role author
author2 Dopico, Berta
Muñoz, Francisco J.
Romo, Silvia
Martín Sánchez, José Ignacio
Labrador, Emilia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cicer arietinum
Elongation
Epicotyls
Galactosidases
Growth
topic Cicer arietinum
Elongation
Epicotyls
Galactosidases
Growth
description The cDNA clone (CanBGal-3) encoding a cell wall pectin- degrading  -galactosidase ( III-Gal) from Cicer arietinum L. cv. Castellana has been identified. The identification was carried out by comparing the deduced amino acid sequences of several isolated chickpea  -galactosidase clones with the purified  III-Gal protein sequence. The expression pattern of the gene corresponding to CanBGal-3 was in concordance with the fluctuations of the enzyme  III-Gal in different seedling organs, being specific to elongating organs such as epicotyls and roots. Transformation of Solanum tuberosum plants with the chickpea CanBGal-3 clone indicated that the  -galactosidase encoded by this clone is a pectin-degrading enzyme. The authors propose an important role for chickpea  III-Gal in pectin degradation in cell walls of vegetative organs such as epicotyls and roots. The degradation of galactan carried out by this enzyme may determine structural changes and affect cell wall porosity. It is suggested that the increase in the size of cell wall pores could permit access of other cell wall-modifying enzymes to their substrate.
publishDate 2003
dc.date.none.fl_str_mv 2003
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/156957
url http://hdl.handle.net/10366/156957
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv CT97 2224
PB98–0290
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869406008665702400
score 15,301603

Similares en LA Referencia