Free Zn2+ determination in systems with Zn-Glutathione

Zn-Glutathione speciation was studied applying the electrochemical technique AGNES (Absence of Gradients and Nernstian Equilibrium Stripping) to determine the free zinc concentration. In titrations varying either pH, total concentration of gluthatione (cT,GSH) or total concentration of Zn (cT,Zn), f...

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Autores: Lao, Mireia, Dago Busquets, Àngela, Serrano, N., Companys Ferran, Encarnació, Puy Llorens, Jaume, Galceran i Nogués, Josep
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/65162
Acceso en línea:https://doi.org/10.1016/j.jelechem.2015.08.029
http://hdl.handle.net/10459.1/65162
Access Level:acceso abierto
Palabra clave:Absence of Gradients and Nernstian Equilibrium Stripping
Speciation techniques
Zinc complexes
Environmental analysis
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spelling Free Zn2+ determination in systems with Zn-GlutathioneLao, MireiaDago Busquets, ÀngelaSerrano, N.Companys Ferran, EncarnacióPuy Llorens, JaumeGalceran i Nogués, JosepAbsence of Gradients and Nernstian Equilibrium StrippingSpeciation techniquesZinc complexesEnvironmental analysisZn-Glutathione speciation was studied applying the electrochemical technique AGNES (Absence of Gradients and Nernstian Equilibrium Stripping) to determine the free zinc concentration. In titrations varying either pH, total concentration of gluthatione (cT,GSH) or total concentration of Zn (cT,Zn), free Zn concentrations determined with AGNES were compared with the values predicted from previously reported complexation constants. The speciation of Zn was studied in a real sample of root extracts of Hordeum vulgare where the cT,Zn had been determined by ICP-MS and cT,GSH by HPLC. The free [Zn2+] was measured with AGNES using a special device where a mixture of N2/CO2 saturated in milliQ water controls the pH and avoids the evaporation of the sample. The lower free zinc concentration determined with AGNES, in comparison with the predicted one assuming the literature complexation constants and taking into account only the presence of Zn and GSH, indicates that it is necessary to include more ligands apart from GSH (as other phytochelatins) in the speciation model.The authors gratefully acknowledge support of this research by the Spanish Ministry Spanish Ministry of Education and Science (Projects CTQ2009-07831, CTM2012-39183, CTQ2012-32863 and CTM2013-48967) and by the "Comissionat d'Universitats i Recerca de la Generalitat de Catalunya” (2014SGR269 and 2014SGR1132). ML acknowledges a FPI grant from the Spanish Ministry.Elsevier2018201820152018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://doi.org/10.1016/j.jelechem.2015.08.029http://hdl.handle.net/10459.1/65162http://hdl.handle.net/10459.1/65162reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/grantAgreement/MICINN//CTQ2009-07831info:eu-repo/grantAgreement/MINECO//CTM2012-39183-C02-02info:eu-repo/grantAgreement/MINECO//CTQ2012-32863info:eu-repo/grantAgreement/MINECO//CTM2013-48967-C2-1-PVersió postprint del document publicat a: https://doi.org/10.1016/j.jelechem.2015.08.029Journal of Electroanalytical Chemistry, 2015, vol. 756, p. 207-211cc-by-nc-nd (c) Elsevier, 2015info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:recercat.cat:10459.1/651622026-05-29T05:05:01Z
dc.title.none.fl_str_mv Free Zn2+ determination in systems with Zn-Glutathione
title Free Zn2+ determination in systems with Zn-Glutathione
spellingShingle Free Zn2+ determination in systems with Zn-Glutathione
Lao, Mireia
Absence of Gradients and Nernstian Equilibrium Stripping
Speciation techniques
Zinc complexes
Environmental analysis
title_short Free Zn2+ determination in systems with Zn-Glutathione
title_full Free Zn2+ determination in systems with Zn-Glutathione
title_fullStr Free Zn2+ determination in systems with Zn-Glutathione
title_full_unstemmed Free Zn2+ determination in systems with Zn-Glutathione
title_sort Free Zn2+ determination in systems with Zn-Glutathione
dc.creator.none.fl_str_mv Lao, Mireia
Dago Busquets, Àngela
Serrano, N.
Companys Ferran, Encarnació
Puy Llorens, Jaume
Galceran i Nogués, Josep
author Lao, Mireia
author_facet Lao, Mireia
Dago Busquets, Àngela
Serrano, N.
Companys Ferran, Encarnació
Puy Llorens, Jaume
Galceran i Nogués, Josep
author_role author
author2 Dago Busquets, Àngela
Serrano, N.
Companys Ferran, Encarnació
Puy Llorens, Jaume
Galceran i Nogués, Josep
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Absence of Gradients and Nernstian Equilibrium Stripping
Speciation techniques
Zinc complexes
Environmental analysis
topic Absence of Gradients and Nernstian Equilibrium Stripping
Speciation techniques
Zinc complexes
Environmental analysis
description Zn-Glutathione speciation was studied applying the electrochemical technique AGNES (Absence of Gradients and Nernstian Equilibrium Stripping) to determine the free zinc concentration. In titrations varying either pH, total concentration of gluthatione (cT,GSH) or total concentration of Zn (cT,Zn), free Zn concentrations determined with AGNES were compared with the values predicted from previously reported complexation constants. The speciation of Zn was studied in a real sample of root extracts of Hordeum vulgare where the cT,Zn had been determined by ICP-MS and cT,GSH by HPLC. The free [Zn2+] was measured with AGNES using a special device where a mixture of N2/CO2 saturated in milliQ water controls the pH and avoids the evaporation of the sample. The lower free zinc concentration determined with AGNES, in comparison with the predicted one assuming the literature complexation constants and taking into account only the presence of Zn and GSH, indicates that it is necessary to include more ligands apart from GSH (as other phytochelatins) in the speciation model.
publishDate 2015
dc.date.none.fl_str_mv 2015
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.jelechem.2015.08.029
http://hdl.handle.net/10459.1/65162
http://hdl.handle.net/10459.1/65162
url https://doi.org/10.1016/j.jelechem.2015.08.029
http://hdl.handle.net/10459.1/65162
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN//CTQ2009-07831
info:eu-repo/grantAgreement/MINECO//CTM2012-39183-C02-02
info:eu-repo/grantAgreement/MINECO//CTQ2012-32863
info:eu-repo/grantAgreement/MINECO//CTM2013-48967-C2-1-P
Versió postprint del document publicat a: https://doi.org/10.1016/j.jelechem.2015.08.029
Journal of Electroanalytical Chemistry, 2015, vol. 756, p. 207-211
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier, 2015
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier, 2015
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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