Characterization of an antennal carboxylesterase from the pest moth Spodoptera littoralis degrading a host plant odorant.

Background: Carboxyl/cholinesterases (CCEs) are highly diversified in insects. These enzymes have a broad range of proposed functions, in neuro/developmental processes, dietary detoxification, insecticide resistance or hormone/pheromone degradation. As few functional data are available on purified o...

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Autores: Durand, Nicolas, Carot Sans, Gerard, Chertemps, Thomas, Bozzolan, Françoise, Party, Virginie, Renou, Michel, Debernard, Stéphane, Rosell Pellisé, Glòria, Maïbèche-Coisne, Martine
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/34071
Acesso em linha:https://hdl.handle.net/2445/34071
Access Level:acceso abierto
Palavra-chave:Lepidòpters
Enzims
Metabolisme de les plantes
Relacions hoste-paràsit
Metabolisme de proteïnes
Esterases
Lepidoptera
Enzymes
Plant metabolism
Host-parasite relationships
Protein metabolism
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repository_id_str
spelling Characterization of an antennal carboxylesterase from the pest moth Spodoptera littoralis degrading a host plant odorant.Durand, NicolasCarot Sans, GerardChertemps, ThomasBozzolan, FrançoiseParty, VirginieRenou, MichelDebernard, StéphaneRosell Pellisé, GlòriaMaïbèche-Coisne, MartineLepidòptersEnzimsMetabolisme de les plantesRelacions hoste-paràsitMetabolisme de proteïnesEsterasesLepidopteraEnzymesPlant metabolismHost-parasite relationshipsProtein metabolismBackground: Carboxyl/cholinesterases (CCEs) are highly diversified in insects. These enzymes have a broad range of proposed functions, in neuro/developmental processes, dietary detoxification, insecticide resistance or hormone/pheromone degradation. As few functional data are available on purified or recombinant CCEs, the physiological role of most of these enzymes is unknown. Concerning their role in olfaction, only two CCEs able to metabolize sex pheromones have been functionally characterized in insects. These enzymes are only expressed in the male antennae, and secreted into the lumen of the pheromone-sensitive sensilla. CCEs able to hydrolyze other odorants than sex pheromones, such as plant volatiles, have not been identified. Methodology: In Spodoptera littoralis, a major crop pest, a diversity of antennal CCEs has been previously identified. We have employed here a combination of molecular biology, biochemistry and electrophysiology approaches to functionally characterize an intracellular CCE, SlCXE10, whose predominant expression in the olfactory sensilla suggested a role in olfaction. A recombinant protein was produced using the baculovirus system and we tested its catabolic properties towards a plant volatile and the sex pheromone components. Conclusion: We showed that SlCXE10 could efficiently hydrolyze a green leaf volatile and to a lesser extent the sex pheromone components. The transcript level in male antennae was also strongly induced by exposure to this plant odorant. In antennae, SlCXE10 expression was associated with sensilla responding to the sex pheromones and to plant odours. These results suggest that a CCE-based intracellular metabolism of odorants could occur in insect antennae, in addition to the extracellular metabolism occurring within the sensillar lumen. This is the first functional characterization of an Odorant- Degrading Enzyme active towards a host plant volatile.Public Library of Science (PLoS)2010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/34071Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0015026PLoS One, 2010, vol. 5, num. 11, p. e15026http://dx.doi.org/10.1371/journal.pone.0015026cc-by (c) Durand, Nicolas et al., 2010http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/340712026-05-27T06:46:51Z
dc.title.none.fl_str_mv Characterization of an antennal carboxylesterase from the pest moth Spodoptera littoralis degrading a host plant odorant.
title Characterization of an antennal carboxylesterase from the pest moth Spodoptera littoralis degrading a host plant odorant.
spellingShingle Characterization of an antennal carboxylesterase from the pest moth Spodoptera littoralis degrading a host plant odorant.
Durand, Nicolas
Lepidòpters
Enzims
Metabolisme de les plantes
Relacions hoste-paràsit
Metabolisme de proteïnes
Esterases
Lepidoptera
Enzymes
Plant metabolism
Host-parasite relationships
Protein metabolism
title_short Characterization of an antennal carboxylesterase from the pest moth Spodoptera littoralis degrading a host plant odorant.
title_full Characterization of an antennal carboxylesterase from the pest moth Spodoptera littoralis degrading a host plant odorant.
title_fullStr Characterization of an antennal carboxylesterase from the pest moth Spodoptera littoralis degrading a host plant odorant.
title_full_unstemmed Characterization of an antennal carboxylesterase from the pest moth Spodoptera littoralis degrading a host plant odorant.
title_sort Characterization of an antennal carboxylesterase from the pest moth Spodoptera littoralis degrading a host plant odorant.
dc.creator.none.fl_str_mv Durand, Nicolas
Carot Sans, Gerard
Chertemps, Thomas
Bozzolan, Françoise
Party, Virginie
Renou, Michel
Debernard, Stéphane
Rosell Pellisé, Glòria
Maïbèche-Coisne, Martine
author Durand, Nicolas
author_facet Durand, Nicolas
Carot Sans, Gerard
Chertemps, Thomas
Bozzolan, Françoise
Party, Virginie
Renou, Michel
Debernard, Stéphane
Rosell Pellisé, Glòria
Maïbèche-Coisne, Martine
author_role author
author2 Carot Sans, Gerard
Chertemps, Thomas
Bozzolan, Françoise
Party, Virginie
Renou, Michel
Debernard, Stéphane
Rosell Pellisé, Glòria
Maïbèche-Coisne, Martine
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Lepidòpters
Enzims
Metabolisme de les plantes
Relacions hoste-paràsit
Metabolisme de proteïnes
Esterases
Lepidoptera
Enzymes
Plant metabolism
Host-parasite relationships
Protein metabolism
topic Lepidòpters
Enzims
Metabolisme de les plantes
Relacions hoste-paràsit
Metabolisme de proteïnes
Esterases
Lepidoptera
Enzymes
Plant metabolism
Host-parasite relationships
Protein metabolism
description Background: Carboxyl/cholinesterases (CCEs) are highly diversified in insects. These enzymes have a broad range of proposed functions, in neuro/developmental processes, dietary detoxification, insecticide resistance or hormone/pheromone degradation. As few functional data are available on purified or recombinant CCEs, the physiological role of most of these enzymes is unknown. Concerning their role in olfaction, only two CCEs able to metabolize sex pheromones have been functionally characterized in insects. These enzymes are only expressed in the male antennae, and secreted into the lumen of the pheromone-sensitive sensilla. CCEs able to hydrolyze other odorants than sex pheromones, such as plant volatiles, have not been identified. Methodology: In Spodoptera littoralis, a major crop pest, a diversity of antennal CCEs has been previously identified. We have employed here a combination of molecular biology, biochemistry and electrophysiology approaches to functionally characterize an intracellular CCE, SlCXE10, whose predominant expression in the olfactory sensilla suggested a role in olfaction. A recombinant protein was produced using the baculovirus system and we tested its catabolic properties towards a plant volatile and the sex pheromone components. Conclusion: We showed that SlCXE10 could efficiently hydrolyze a green leaf volatile and to a lesser extent the sex pheromone components. The transcript level in male antennae was also strongly induced by exposure to this plant odorant. In antennae, SlCXE10 expression was associated with sensilla responding to the sex pheromones and to plant odours. These results suggest that a CCE-based intracellular metabolism of odorants could occur in insect antennae, in addition to the extracellular metabolism occurring within the sensillar lumen. This is the first functional characterization of an Odorant- Degrading Enzyme active towards a host plant volatile.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/34071
url https://hdl.handle.net/2445/34071
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0015026
PLoS One, 2010, vol. 5, num. 11, p. e15026
http://dx.doi.org/10.1371/journal.pone.0015026
dc.rights.none.fl_str_mv cc-by (c) Durand, Nicolas et al., 2010
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Durand, Nicolas et al., 2010
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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