A Key Role of the EMC Complex for Mitochondrial Respiration and Quiescence in Fission Yeasts

In eukaryotes, oxygen consumption is mainly driven by the respiratory activity of mitochondria, which generates most of the cellular energy that sustains life. This parameter provides direct information about mitochondrial activity of all aerobic biological systems. Using the Seahorse analyzer instr...

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Detalles Bibliográficos
Autores: Berraquero, Modesto, Álvarez Tallada, Víctor, Jiménez, Juan
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad Pablo de Olavide (UPO)
Repositorio:RIO. Repositorio Institucional Olavide
Idioma:inglés
OAI Identifier:oai:dnet:rio_________::af52043c547850217329c3e80656d9aa
Acceso en línea:https://hdl.handle.net/10433/26633
Access Level:acceso abierto
Palabra clave:EMC complex
Ergosterol
Fission yeast
Mitochondria
Oxygen consumption
Quiescence
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spelling A Key Role of the EMC Complex for Mitochondrial Respiration and Quiescence in Fission YeastsBerraquero, ModestoÁlvarez Tallada, VíctorJiménez, JuanEMC complexErgosterolFission yeastMitochondriaOxygen consumptionQuiescenceIn eukaryotes, oxygen consumption is mainly driven by the respiratory activity of mitochondria, which generates most of the cellular energy that sustains life. This parameter provides direct information about mitochondrial activity of all aerobic biological systems. Using the Seahorse analyzer instrument, we show here that deletion of the oca3/emc2 gene (oca3Δ) encoding the Emc2 subunit of the ER membrane complex (EMC), a conserved chaperone/insertase that aids membrane protein biogenesis in the ER, severely affects oxygen consumption rates and quiescence survival in Schizosaccharomyces pombe yeast cells. Remarkably, the respiratory defect of the oca3Δ mutation (EMC dysfunction) is rescued synergistically by disruption of ergosterol biosynthesis (erg5Δ) and the action of the membrane fluidizing agent tween 20, suggesting a direct role of membrane fluidity and sterol composition in mitochondrial respiration in the fission yeast.Wiley20262026-05-2020252025-04-0120252025-04-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10433/26633reponame:RIO. Repositorio Institucional Olavideinstname:Universidad Pablo de Olavide (UPO)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:dnet:rio_________::af52043c547850217329c3e80656d9aa2026-06-13T12:46:27Z
dc.title.none.fl_str_mv A Key Role of the EMC Complex for Mitochondrial Respiration and Quiescence in Fission Yeasts
title A Key Role of the EMC Complex for Mitochondrial Respiration and Quiescence in Fission Yeasts
spellingShingle A Key Role of the EMC Complex for Mitochondrial Respiration and Quiescence in Fission Yeasts
Berraquero, Modesto
EMC complex
Ergosterol
Fission yeast
Mitochondria
Oxygen consumption
Quiescence
title_short A Key Role of the EMC Complex for Mitochondrial Respiration and Quiescence in Fission Yeasts
title_full A Key Role of the EMC Complex for Mitochondrial Respiration and Quiescence in Fission Yeasts
title_fullStr A Key Role of the EMC Complex for Mitochondrial Respiration and Quiescence in Fission Yeasts
title_full_unstemmed A Key Role of the EMC Complex for Mitochondrial Respiration and Quiescence in Fission Yeasts
title_sort A Key Role of the EMC Complex for Mitochondrial Respiration and Quiescence in Fission Yeasts
dc.creator.none.fl_str_mv Berraquero, Modesto
Álvarez Tallada, Víctor
Jiménez, Juan
author Berraquero, Modesto
author_facet Berraquero, Modesto
Álvarez Tallada, Víctor
Jiménez, Juan
author_role author
author2 Álvarez Tallada, Víctor
Jiménez, Juan
author2_role author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv EMC complex
Ergosterol
Fission yeast
Mitochondria
Oxygen consumption
Quiescence
topic EMC complex
Ergosterol
Fission yeast
Mitochondria
Oxygen consumption
Quiescence
description In eukaryotes, oxygen consumption is mainly driven by the respiratory activity of mitochondria, which generates most of the cellular energy that sustains life. This parameter provides direct information about mitochondrial activity of all aerobic biological systems. Using the Seahorse analyzer instrument, we show here that deletion of the oca3/emc2 gene (oca3Δ) encoding the Emc2 subunit of the ER membrane complex (EMC), a conserved chaperone/insertase that aids membrane protein biogenesis in the ER, severely affects oxygen consumption rates and quiescence survival in Schizosaccharomyces pombe yeast cells. Remarkably, the respiratory defect of the oca3Δ mutation (EMC dysfunction) is rescued synergistically by disruption of ergosterol biosynthesis (erg5Δ) and the action of the membrane fluidizing agent tween 20, suggesting a direct role of membrane fluidity and sterol composition in mitochondrial respiration in the fission yeast.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-04-01
2025
2025-04-01
2026
2026-05-20
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10433/26633
url https://hdl.handle.net/10433/26633
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:RIO. Repositorio Institucional Olavide
instname:Universidad Pablo de Olavide (UPO)
instname_str Universidad Pablo de Olavide (UPO)
reponame_str RIO. Repositorio Institucional Olavide
collection RIO. Repositorio Institucional Olavide
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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