A manipulation of carotenoid metabolism influences biomass partitioning and fitness in tomato

[EN] Improving yield, nutritional value and tolerance to abiotic stress are major targets of current breeding and biotechnological approaches that aim at increasing crop production and ensuring food security. Metabolic engineering of carotenoids, the precursor of vitamin-A and plant hormones that re...

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Autores: Mi, Jianing, Vallarino, Jose G., Petrik, Ivan, Novak, Ondrej, Correa, Sandra M., Chodasiewicz, Monika, Havaux, Michel, Al-Babili, Salim, Fernie, Alisdair R., Skirycz, Aleksandra, Moreno, Juan C., RODRIGUEZ-CONCEPCION, Manuel|||0000-0002-1280-2305
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/198244
Acceso en línea:https://riunet.upv.es/handle/10251/198244
Access Level:acceso abierto
Palabra clave:Abiotic stress tolerance
Apocarotenoids
Biomass and yield
Carotenoids
Metabolic engineering
Metabolites and lipids
Phytohormones
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oai_identifier_str oai:riunet.upv.es:10251/198244
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv A manipulation of carotenoid metabolism influences biomass partitioning and fitness in tomato
title A manipulation of carotenoid metabolism influences biomass partitioning and fitness in tomato
spellingShingle A manipulation of carotenoid metabolism influences biomass partitioning and fitness in tomato
Mi, Jianing
Abiotic stress tolerance
Apocarotenoids
Biomass and yield
Carotenoids
Metabolic engineering
Metabolites and lipids
Phytohormones
title_short A manipulation of carotenoid metabolism influences biomass partitioning and fitness in tomato
title_full A manipulation of carotenoid metabolism influences biomass partitioning and fitness in tomato
title_fullStr A manipulation of carotenoid metabolism influences biomass partitioning and fitness in tomato
title_full_unstemmed A manipulation of carotenoid metabolism influences biomass partitioning and fitness in tomato
title_sort A manipulation of carotenoid metabolism influences biomass partitioning and fitness in tomato
dc.creator.none.fl_str_mv Mi, Jianing
Vallarino, Jose G.
Petrik, Ivan
Novak, Ondrej
Correa, Sandra M.
Chodasiewicz, Monika
Havaux, Michel
Al-Babili, Salim
Fernie, Alisdair R.
Skirycz, Aleksandra
Moreno, Juan C.
RODRIGUEZ-CONCEPCION, Manuel|||0000-0002-1280-2305
author Mi, Jianing
author_facet Mi, Jianing
Vallarino, Jose G.
Petrik, Ivan
Novak, Ondrej
Correa, Sandra M.
Chodasiewicz, Monika
Havaux, Michel
Al-Babili, Salim
Fernie, Alisdair R.
Skirycz, Aleksandra
Moreno, Juan C.
RODRIGUEZ-CONCEPCION, Manuel|||0000-0002-1280-2305
author_role author
author2 Vallarino, Jose G.
Petrik, Ivan
Novak, Ondrej
Correa, Sandra M.
Chodasiewicz, Monika
Havaux, Michel
Al-Babili, Salim
Fernie, Alisdair R.
Skirycz, Aleksandra
Moreno, Juan C.
RODRIGUEZ-CONCEPCION, Manuel|||0000-0002-1280-2305
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Palacký University Olomouc
Agencia Estatal de Investigación
European Regional Development Fund
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Abiotic stress tolerance
Apocarotenoids
Biomass and yield
Carotenoids
Metabolic engineering
Metabolites and lipids
Phytohormones
topic Abiotic stress tolerance
Apocarotenoids
Biomass and yield
Carotenoids
Metabolic engineering
Metabolites and lipids
Phytohormones
description [EN] Improving yield, nutritional value and tolerance to abiotic stress are major targets of current breeding and biotechnological approaches that aim at increasing crop production and ensuring food security. Metabolic engineering of carotenoids, the precursor of vitamin-A and plant hormones that regulate plant growth and response to adverse growth conditions, has been mainly focusing on provitamin A biofortification or the production of high-value carotenoids. Here, we show that the introduction of a single gene of the carotenoid biosynthetic pathway in different tomato cultivars induced profound metabolic alterations in carotenoid, apocarotenoid and phytohormones pathways. Alterations in isoprenoid-(abscisic acid, gibberellins, cytokinins) and non-isoprenoid (auxin and jasmonic acid) derived hormones together with enhanced xanthophyll content influenced biomass partitioning and abiotic stress tolerance (high light, salt, and drought), and it caused an up to 77% fruit yield increase and enhanced fruit's provitamin A content. In addition, metabolic and hormonal changes led to accumulation of key primary metabolites (e.g. osmoprotectants and antiaging agents) contributing with enhanced abiotic stress tolerance and fruit shelf life. Our findings pave the way for developing a new generation of crops that combine high productivity and increased nutritional value with the capability to cope with climate change related environmental challenges.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-03-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/198244
url https://riunet.upv.es/handle/10251/198244
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv MEYS MEYS CZ.02.1.01%2F0.0%2F0.0%2F16_019%2F0000827
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 BIO2017-84041-P NUEVAS HERRAMIENTAS BIOTECNOLOGICAS PARA MEJORAR LA PRODUCCION Y EL ALMACENAJE DE VITAMINAS A Y E EN CELULAS VEGETALES
Palacký University Olomouc Palacký University Olomouc IGA_PrF_2021_011
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling A manipulation of carotenoid metabolism influences biomass partitioning and fitness in tomatoMi, JianingVallarino, Jose G.Petrik, IvanNovak, OndrejCorrea, Sandra M.Chodasiewicz, MonikaHavaux, MichelAl-Babili, SalimFernie, Alisdair R.Skirycz, AleksandraMoreno, Juan C.RODRIGUEZ-CONCEPCION, Manuel|||0000-0002-1280-2305Abiotic stress toleranceApocarotenoidsBiomass and yieldCarotenoidsMetabolic engineeringMetabolites and lipidsPhytohormones[EN] Improving yield, nutritional value and tolerance to abiotic stress are major targets of current breeding and biotechnological approaches that aim at increasing crop production and ensuring food security. Metabolic engineering of carotenoids, the precursor of vitamin-A and plant hormones that regulate plant growth and response to adverse growth conditions, has been mainly focusing on provitamin A biofortification or the production of high-value carotenoids. Here, we show that the introduction of a single gene of the carotenoid biosynthetic pathway in different tomato cultivars induced profound metabolic alterations in carotenoid, apocarotenoid and phytohormones pathways. Alterations in isoprenoid-(abscisic acid, gibberellins, cytokinins) and non-isoprenoid (auxin and jasmonic acid) derived hormones together with enhanced xanthophyll content influenced biomass partitioning and abiotic stress tolerance (high light, salt, and drought), and it caused an up to 77% fruit yield increase and enhanced fruit's provitamin A content. In addition, metabolic and hormonal changes led to accumulation of key primary metabolites (e.g. osmoprotectants and antiaging agents) contributing with enhanced abiotic stress tolerance and fruit shelf life. Our findings pave the way for developing a new generation of crops that combine high productivity and increased nutritional value with the capability to cope with climate change related environmental challenges.We are grateful to Prof. Dr. Lothar Willmitzer for his support and advice. We thank Prof. Dr. Ralph Bock (Max Planck Institute of Molecular Plant physiology, Golm, Germany) and Dr. Caterina D'Ambrosio (Centro Ricerche Metapontum Agrobios, ALSIA, Italy) for kindly providing the transplastomic pNLyc#2 and LCe seeds and the homozygous nuclear High Caro (H.C.) lines, respectively. We thank Dr. Camila Caldana and Anne Michaelis for providing the GC facility and running the GC samples, respectively, and Maria Rosa Rodriguez-Goberna for technical support related with pigment analysis (supported by grant BIO2017-84041-P from the Spanish AEI). In addition, we thank Hana Martinkov ' a and Petra Amakorova for their help with phytohormone analyses. The hormonomics work was funded by the Ministry of Education, Youth and Sports of the Czech Republic (European Regional Development Fund-Project "Plants as a tool for sustainable global development" No. CZ.02.1.01/0.0/0.0/16_019/0000827), and the Internal Grant Agency of Palacky University (IGA_PrF_2021_011).ElsevierInstituto Universitario Mixto de Biología Molecular y Celular de PlantasPalacký University OlomoucAgencia Estatal de InvestigaciónEuropean Regional Development FundRepositorio Institucional de la Universitat Politècnica de València Riunet20222022-03-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/198244reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMEYS MEYS CZ.02.1.01%2F0.0%2F0.0%2F16_019%2F0000827Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 BIO2017-84041-P NUEVAS HERRAMIENTAS BIOTECNOLOGICAS PARA MEJORAR LA PRODUCCION Y EL ALMACENAJE DE VITAMINAS A Y E EN CELULAS VEGETALESPalacký University Olomouc Palacký University Olomouc IGA_PrF_2021_011open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1982442026-06-13T07:49:27Z
score 15,301629

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