Phosphomonoesterases and cholinesterases from Taenia pisiformis cysticerci
Phosphohydrolase and cholinesterase activities were found in the cytosolic fraction of Taenia pisiformis cysticerci from naturally infected hares. By using p-nitrophenyl phosphate as substrate, mainly acid (pH 5:0) but also alkaline (pH 10.0) phosphatase activities were quantified. Cholinesterase ac...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2004 |
| País: | España |
| Institución: | Universidad de Alcalá (UAH) |
| Repositorio: | e_Buah Biblioteca Digital Universidad de Alcalá |
| Idioma: | inglés |
| OAI Identifier: | oai:ebuah.uah.es:10017/61192 |
| Acceso en línea: | http://hdl.handle.net/10017/61192 |
| Access Level: | acceso abierto |
| Palabra clave: | acid phosphatase alkaline phosphatase acetylcholinesterase metacestode Taenia pisiformis Medicina Medicine |
| Sumario: | Phosphohydrolase and cholinesterase activities were found in the cytosolic fraction of Taenia pisiformis cysticerci from naturally infected hares. By using p-nitrophenyl phosphate as substrate, mainly acid (pH 5:0) but also alkaline (pH 10.0) phosphatase activities were quantified. Cholinesterase activity was determined in the presence of acetylthiocholine iodide. Both hydrolases were also detected by native poliacrylamide gel electrophoresis, and preliminary characterized by inhibition assays. The possible biological roles of these enzymes in cestodes are discussed. |
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