Transposon insertion in the purL gene induces biofilm depletion in Escherichia coli ATCC 25922

Current Escherichia coli antibiofilm treatments comprise a combination of antibiotics commonly used against planktonic cells, leading to treatment failure. A better understanding of the genes involved in biofilm formation could facilitate the development of efficient and specific new antibiofilm tre...

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Autores: Cepas, Virginio, Ballén, Victoria, Gabasa, Yaiza, Ramírez, Miriam, López, Yuly, Soto González, Sara M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/176419
Acceso en línea:https://hdl.handle.net/2445/176419
Access Level:acceso abierto
Palabra clave:Biofilms
Escheríchia coli
Antibiòtics
Escherichia coli
Antibiotics
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spelling Transposon insertion in the purL gene induces biofilm depletion in Escherichia coli ATCC 25922Cepas, VirginioBallén, VictoriaGabasa, YaizaRamírez, MiriamLópez, YulySoto González, Sara M.BiofilmsEscheríchia coliAntibiòticsBiofilmsEscherichia coliAntibioticsCurrent Escherichia coli antibiofilm treatments comprise a combination of antibiotics commonly used against planktonic cells, leading to treatment failure. A better understanding of the genes involved in biofilm formation could facilitate the development of efficient and specific new antibiofilm treatments. A total of 2578 E. coli mutants were generated by transposon insertion, of which 536 were analysed in this study. After sequencing, Tn263 mutant, classified as low biofilm-former (LF) compared to the wild-type (wt) strain (ATCC 25922), showed an interruption in the purL gene, involved in the de novo purine biosynthesis pathway. To elucidate the role of purL in biofilm formation, a knockout was generated showing reduced production of curli fibres, leading to an impaired biofilm formation. These conditions were restored by complementation of the strain or addition of exogenous inosine. Proteomic and transcriptional analyses were performed to characterise the differences caused by purL alterations. Thirteen proteins were altered compared to wt. The corresponding genes were analysed by qRT-PCR not only in the Tn263 and wt, but also in clinical strains with different biofilm activity. Overall, this study suggests that purL is essential for biofilm formation in E. coli and can be considered as a potential antibiofilm target.MDPI2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/176419Articles publicats en revistes (Fonaments Clínics)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/pathogens9090774Pathogens, 2020, vol. 9, num. 9https://doi.org/10.3390/pathogens9090774cc-by (c) Cepas, Virginio et al., 2020http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1764192026-05-27T06:46:51Z
dc.title.none.fl_str_mv Transposon insertion in the purL gene induces biofilm depletion in Escherichia coli ATCC 25922
title Transposon insertion in the purL gene induces biofilm depletion in Escherichia coli ATCC 25922
spellingShingle Transposon insertion in the purL gene induces biofilm depletion in Escherichia coli ATCC 25922
Cepas, Virginio
Biofilms
Escheríchia coli
Antibiòtics
Biofilms
Escherichia coli
Antibiotics
title_short Transposon insertion in the purL gene induces biofilm depletion in Escherichia coli ATCC 25922
title_full Transposon insertion in the purL gene induces biofilm depletion in Escherichia coli ATCC 25922
title_fullStr Transposon insertion in the purL gene induces biofilm depletion in Escherichia coli ATCC 25922
title_full_unstemmed Transposon insertion in the purL gene induces biofilm depletion in Escherichia coli ATCC 25922
title_sort Transposon insertion in the purL gene induces biofilm depletion in Escherichia coli ATCC 25922
dc.creator.none.fl_str_mv Cepas, Virginio
Ballén, Victoria
Gabasa, Yaiza
Ramírez, Miriam
López, Yuly
Soto González, Sara M.
author Cepas, Virginio
author_facet Cepas, Virginio
Ballén, Victoria
Gabasa, Yaiza
Ramírez, Miriam
López, Yuly
Soto González, Sara M.
author_role author
author2 Ballén, Victoria
Gabasa, Yaiza
Ramírez, Miriam
López, Yuly
Soto González, Sara M.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biofilms
Escheríchia coli
Antibiòtics
Biofilms
Escherichia coli
Antibiotics
topic Biofilms
Escheríchia coli
Antibiòtics
Biofilms
Escherichia coli
Antibiotics
description Current Escherichia coli antibiofilm treatments comprise a combination of antibiotics commonly used against planktonic cells, leading to treatment failure. A better understanding of the genes involved in biofilm formation could facilitate the development of efficient and specific new antibiofilm treatments. A total of 2578 E. coli mutants were generated by transposon insertion, of which 536 were analysed in this study. After sequencing, Tn263 mutant, classified as low biofilm-former (LF) compared to the wild-type (wt) strain (ATCC 25922), showed an interruption in the purL gene, involved in the de novo purine biosynthesis pathway. To elucidate the role of purL in biofilm formation, a knockout was generated showing reduced production of curli fibres, leading to an impaired biofilm formation. These conditions were restored by complementation of the strain or addition of exogenous inosine. Proteomic and transcriptional analyses were performed to characterise the differences caused by purL alterations. Thirteen proteins were altered compared to wt. The corresponding genes were analysed by qRT-PCR not only in the Tn263 and wt, but also in clinical strains with different biofilm activity. Overall, this study suggests that purL is essential for biofilm formation in E. coli and can be considered as a potential antibiofilm target.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/176419
url https://hdl.handle.net/2445/176419
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/pathogens9090774
Pathogens, 2020, vol. 9, num. 9
https://doi.org/10.3390/pathogens9090774
dc.rights.none.fl_str_mv cc-by (c) Cepas, Virginio et al., 2020
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Cepas, Virginio et al., 2020
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Fonaments Clínics)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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