Multiserotype Protection Elicited by a Combinatorial Prime-Boost Vaccination Strategy against Bluetongue Virus

[EN]Bluetongue virus (BTV) belongs to the genus Orbivirus within the family Reoviridae. The development of vector-based vaccines expressing conserved protective antigens results in increased immune activation and could reduce the number of multiserotype vaccinations required, therefore providing a c...

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Autores: Calvo Pinilla, Eva, Navasa Mayo, Nicolás, Anguita, Juan, Ortego, Javier
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:España
Recursos:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/24862
Acesso em linha:https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0034735
https://hdl.handle.net/10612/24862
Access Level:acceso abierto
Palavra-chave:Biología
Bluetongue virus (BTV)
Orbivirus
Vector-based vaccines
Virus de la lengua azul (BTV)
Vacunas basadas en vectores
2302.21 Biología Molecular
3109.11 Virología
3109.03 Inmunología
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spelling Multiserotype Protection Elicited by a Combinatorial Prime-Boost Vaccination Strategy against Bluetongue VirusCalvo Pinilla, EvaNavasa Mayo, NicolásAnguita, JuanOrtego, JavierBiologíaBluetongue virus (BTV)OrbivirusVector-based vaccinesVirus de la lengua azul (BTV)Vacunas basadas en vectores2302.21 Biología Molecular3109.11 Virología3109.03 Inmunología[EN]Bluetongue virus (BTV) belongs to the genus Orbivirus within the family Reoviridae. The development of vector-based vaccines expressing conserved protective antigens results in increased immune activation and could reduce the number of multiserotype vaccinations required, therefore providing a cost-effective product. Recent recombinant DNA technology has allowed the development of novel strategies to develop marker and safe vaccines against BTV. We have now engineered naked DNAs and recombinant modified vaccinia virus Ankara (rMVA) expressing VP2, VP7 and NS1 proteins from BTV-4. IFNAR (-/-) mice inoculated with DNA/rMVA-VP2,-VP7-NS1 in an heterologous prime boost vaccination strategy generated significant levels of antibodies specific of VP2, VP7, and NS1, including those with neutralizing activity against BTV-4. In addition, vaccination stimulated specific CD8 + T cell responses against these three BTV proteins. Importantly, the vaccine combination expressing NS1, VP2 and VP7 proteins of BTV-4, elicited sterile protection against a lethal dose of homologous BTV-4 infection. Remarkably, the vaccine induced cross-protection against lethal doses of heterologous BTV-8 and BTV-1 suggesting that the DNA/rMVA-VP2,-VP7,-NS1 marker vaccine is a promising multiserotype vaccine against BTV.SIThis work was supported by grants AGL2008-00646/GAN and AGL2011-23506/GAN from the Comisión Interministerial de Ciencia y Tecnología (CICYT), 228394-NADIR-Integrating Activities 7th European Union program, and the European Union Network of Excellence EPIZONE (Contract No FOOD-CT-2006-016236).Public Library of ScienceBioquimica y Biologia MolecularFacultad de Veterinaria2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0034735https://hdl.handle.net/10612/24862reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónIngléshttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/248622026-06-24T12:43:27Z
dc.title.none.fl_str_mv Multiserotype Protection Elicited by a Combinatorial Prime-Boost Vaccination Strategy against Bluetongue Virus
title Multiserotype Protection Elicited by a Combinatorial Prime-Boost Vaccination Strategy against Bluetongue Virus
spellingShingle Multiserotype Protection Elicited by a Combinatorial Prime-Boost Vaccination Strategy against Bluetongue Virus
Calvo Pinilla, Eva
Biología
Bluetongue virus (BTV)
Orbivirus
Vector-based vaccines
Virus de la lengua azul (BTV)
Vacunas basadas en vectores
2302.21 Biología Molecular
3109.11 Virología
3109.03 Inmunología
title_short Multiserotype Protection Elicited by a Combinatorial Prime-Boost Vaccination Strategy against Bluetongue Virus
title_full Multiserotype Protection Elicited by a Combinatorial Prime-Boost Vaccination Strategy against Bluetongue Virus
title_fullStr Multiserotype Protection Elicited by a Combinatorial Prime-Boost Vaccination Strategy against Bluetongue Virus
title_full_unstemmed Multiserotype Protection Elicited by a Combinatorial Prime-Boost Vaccination Strategy against Bluetongue Virus
title_sort Multiserotype Protection Elicited by a Combinatorial Prime-Boost Vaccination Strategy against Bluetongue Virus
dc.creator.none.fl_str_mv Calvo Pinilla, Eva
Navasa Mayo, Nicolás
Anguita, Juan
Ortego, Javier
author Calvo Pinilla, Eva
author_facet Calvo Pinilla, Eva
Navasa Mayo, Nicolás
Anguita, Juan
Ortego, Javier
author_role author
author2 Navasa Mayo, Nicolás
Anguita, Juan
Ortego, Javier
author2_role author
author
author
dc.contributor.none.fl_str_mv Bioquimica y Biologia Molecular
Facultad de Veterinaria
dc.subject.none.fl_str_mv Biología
Bluetongue virus (BTV)
Orbivirus
Vector-based vaccines
Virus de la lengua azul (BTV)
Vacunas basadas en vectores
2302.21 Biología Molecular
3109.11 Virología
3109.03 Inmunología
topic Biología
Bluetongue virus (BTV)
Orbivirus
Vector-based vaccines
Virus de la lengua azul (BTV)
Vacunas basadas en vectores
2302.21 Biología Molecular
3109.11 Virología
3109.03 Inmunología
description [EN]Bluetongue virus (BTV) belongs to the genus Orbivirus within the family Reoviridae. The development of vector-based vaccines expressing conserved protective antigens results in increased immune activation and could reduce the number of multiserotype vaccinations required, therefore providing a cost-effective product. Recent recombinant DNA technology has allowed the development of novel strategies to develop marker and safe vaccines against BTV. We have now engineered naked DNAs and recombinant modified vaccinia virus Ankara (rMVA) expressing VP2, VP7 and NS1 proteins from BTV-4. IFNAR (-/-) mice inoculated with DNA/rMVA-VP2,-VP7-NS1 in an heterologous prime boost vaccination strategy generated significant levels of antibodies specific of VP2, VP7, and NS1, including those with neutralizing activity against BTV-4. In addition, vaccination stimulated specific CD8 + T cell responses against these three BTV proteins. Importantly, the vaccine combination expressing NS1, VP2 and VP7 proteins of BTV-4, elicited sterile protection against a lethal dose of homologous BTV-4 infection. Remarkably, the vaccine induced cross-protection against lethal doses of heterologous BTV-8 and BTV-1 suggesting that the DNA/rMVA-VP2,-VP7,-NS1 marker vaccine is a promising multiserotype vaccine against BTV.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0034735
https://hdl.handle.net/10612/24862
url https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0034735
https://hdl.handle.net/10612/24862
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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