Surfactant-Free β-Galactosidase Micromotors for “On-The-Move” Lactose Hydrolysis

Surfactant-free β-galactosidase micromotors are explored here as moving biocatalyst for highly efficient lactose hydrolysis from raw milk. The coupling of the hydrolytic properties of such enzyme with the efficient movement of carbon nanotube tubular micromotors results in nearly 100% lactose hydrol...

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Detalles Bibliográficos
Autores: Jurado Sánchez, Beatriz, Escarpa, Alberto, María Hormigos, Roberto
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad Nacional de Educación a Distancia
Repositorio:e-spacio. Repositorio Institucional de la UNED
Idioma:inglés
OAI Identifier:oai:e-spacio.uned.es:20.500.14468/11579
Acceso en línea:https://hdl.handle.net/20.500.14468/11579
Access Level:acceso abierto
Palabra clave:β-galactosidase
lactose
micromotors
milk
self-propulsion
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spelling Surfactant-Free β-Galactosidase Micromotors for “On-The-Move” Lactose HydrolysisJurado Sánchez, BeatrizEscarpa, AlbertoMaría Hormigos, Robertoβ-galactosidaselactosemicromotorsmilkself-propulsionSurfactant-free β-galactosidase micromotors are explored here as moving biocatalyst for highly efficient lactose hydrolysis from raw milk. The coupling of the hydrolytic properties of such enzyme with the efficient movement of carbon nanotube tubular micromotors results in nearly 100% lactose hydrolysis and two fold removal efficiency as compared with static conditions and with free enzyme. The incorporation of an inner Ni layer allows its reusability to operate in batch mode. The rough micromotor surface area allows the immobilization of a high loading of β-galactosidase and results in an increase in the enzyme affinity toward lactose. The new micromotor concept opens new avenues for the use of micromotors as moving immobilized biocatalyst to improve the technological process not only in food industry but also in other fields.Wiley Online Librarye-Spacio UNED20242024-05-2020182018-01-0120182018-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14468/11579reponame:e-spacio. Repositorio Institucional de la UNEDinstname:Universidad Nacional de Educación a DistanciaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0oai:e-spacio.uned.es:20.500.14468/115792026-06-06T12:38:31Z
dc.title.none.fl_str_mv Surfactant-Free β-Galactosidase Micromotors for “On-The-Move” Lactose Hydrolysis
title Surfactant-Free β-Galactosidase Micromotors for “On-The-Move” Lactose Hydrolysis
spellingShingle Surfactant-Free β-Galactosidase Micromotors for “On-The-Move” Lactose Hydrolysis
Jurado Sánchez, Beatriz
β-galactosidase
lactose
micromotors
milk
self-propulsion
title_short Surfactant-Free β-Galactosidase Micromotors for “On-The-Move” Lactose Hydrolysis
title_full Surfactant-Free β-Galactosidase Micromotors for “On-The-Move” Lactose Hydrolysis
title_fullStr Surfactant-Free β-Galactosidase Micromotors for “On-The-Move” Lactose Hydrolysis
title_full_unstemmed Surfactant-Free β-Galactosidase Micromotors for “On-The-Move” Lactose Hydrolysis
title_sort Surfactant-Free β-Galactosidase Micromotors for “On-The-Move” Lactose Hydrolysis
dc.creator.none.fl_str_mv Jurado Sánchez, Beatriz
Escarpa, Alberto
María Hormigos, Roberto
author Jurado Sánchez, Beatriz
author_facet Jurado Sánchez, Beatriz
Escarpa, Alberto
María Hormigos, Roberto
author_role author
author2 Escarpa, Alberto
María Hormigos, Roberto
author2_role author
author
dc.contributor.none.fl_str_mv e-Spacio UNED
dc.subject.none.fl_str_mv β-galactosidase
lactose
micromotors
milk
self-propulsion
topic β-galactosidase
lactose
micromotors
milk
self-propulsion
description Surfactant-free β-galactosidase micromotors are explored here as moving biocatalyst for highly efficient lactose hydrolysis from raw milk. The coupling of the hydrolytic properties of such enzyme with the efficient movement of carbon nanotube tubular micromotors results in nearly 100% lactose hydrolysis and two fold removal efficiency as compared with static conditions and with free enzyme. The incorporation of an inner Ni layer allows its reusability to operate in batch mode. The rough micromotor surface area allows the immobilization of a high loading of β-galactosidase and results in an increase in the enzyme affinity toward lactose. The new micromotor concept opens new avenues for the use of micromotors as moving immobilized biocatalyst to improve the technological process not only in food industry but also in other fields.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01
2018
2018-01-01
2024
2024-05-20
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14468/11579
url https://hdl.handle.net/20.500.14468/11579
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley Online Library
publisher.none.fl_str_mv Wiley Online Library
dc.source.none.fl_str_mv reponame:e-spacio. Repositorio Institucional de la UNED
instname:Universidad Nacional de Educación a Distancia
instname_str Universidad Nacional de Educación a Distancia
reponame_str e-spacio. Repositorio Institucional de la UNED
collection e-spacio. Repositorio Institucional de la UNED
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405873178148864
score 15,811543

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