Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni

Campylobacter, a major cause of food-borne gastroenteritis worldwide, colonize the gastrointestinal tract of a wide range of animals, being birds the main reservoir. The mechanisms involved in the interaction of Campylobacter with the different hosts are poorly understood. The cytolethal distending...

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Autores: Guirado, P, Iglesias-Torrens, Y, Miro, E, Navarro, F, Attolini, CSO, Balsalobre, C, Madrid, C
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
Repositorio:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
OAI Identifier:oai:iibsantpau.fundanetsuite.com:p13033
Acceso en línea:https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=13033
http://hdl.handle.net/2445/194059
Access Level:acceso abierto
Palabra clave:allelic variability
Campylobacter
cytolethal distending toxin
PCR detection
WGS
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spelling Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuniGuirado, PIglesias-Torrens, YMiro, ENavarro, FAttolini, CSOBalsalobre, CMadrid, Callelic variabilityCampylobactercytolethal distending toxinPCR detectionWGSCampylobacter, a major cause of food-borne gastroenteritis worldwide, colonize the gastrointestinal tract of a wide range of animals, being birds the main reservoir. The mechanisms involved in the interaction of Campylobacter with the different hosts are poorly understood. The cytolethal distending toxin, encoded in the cdtABC operon, is considered a pivotal virulence factor during human infection. Differences in the prevalence of cdtABC genes in Campylobacter isolates from three distinct origins (wild birds, broiler chickens and humans) prompted us to further characterize their allelic variability. The sequence of cdtABC is highly conserved among broiler and human isolates. A high diversity of cdtABC alleles was found among wild bird isolates, including several alleles that do not produce any functional CDT. These results suggest that specific variants of the cdtABC operon might define the host range of specific Campylobacter jejuni isolates. Moreover, our data indicate that PCR methodology is inaccurate to characterize the prevalence of the cdt genes, since negative PCR detection can be the result of divergences in the sequence used for primer design rather than indicating the absence of a specific gene.WILEY2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=13033http://hdl.handle.net/2445/194059Zoonoses and Public HealthISSN: 18631959ISSNe: 18632378reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pauinstname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)Inglésinfo:eu-repo/semantics/openAccessoai:iibsantpau.fundanetsuite.com:p130332026-06-14T12:41:47Z
dc.title.none.fl_str_mv Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni
title Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni
spellingShingle Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni
Guirado, P
allelic variability
Campylobacter
cytolethal distending toxin
PCR detection
WGS
title_short Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni
title_full Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni
title_fullStr Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni
title_full_unstemmed Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni
title_sort Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni
dc.creator.none.fl_str_mv Guirado, P
Iglesias-Torrens, Y
Miro, E
Navarro, F
Attolini, CSO
Balsalobre, C
Madrid, C
author Guirado, P
author_facet Guirado, P
Iglesias-Torrens, Y
Miro, E
Navarro, F
Attolini, CSO
Balsalobre, C
Madrid, C
author_role author
author2 Iglesias-Torrens, Y
Miro, E
Navarro, F
Attolini, CSO
Balsalobre, C
Madrid, C
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv allelic variability
Campylobacter
cytolethal distending toxin
PCR detection
WGS
topic allelic variability
Campylobacter
cytolethal distending toxin
PCR detection
WGS
description Campylobacter, a major cause of food-borne gastroenteritis worldwide, colonize the gastrointestinal tract of a wide range of animals, being birds the main reservoir. The mechanisms involved in the interaction of Campylobacter with the different hosts are poorly understood. The cytolethal distending toxin, encoded in the cdtABC operon, is considered a pivotal virulence factor during human infection. Differences in the prevalence of cdtABC genes in Campylobacter isolates from three distinct origins (wild birds, broiler chickens and humans) prompted us to further characterize their allelic variability. The sequence of cdtABC is highly conserved among broiler and human isolates. A high diversity of cdtABC alleles was found among wild bird isolates, including several alleles that do not produce any functional CDT. These results suggest that specific variants of the cdtABC operon might define the host range of specific Campylobacter jejuni isolates. Moreover, our data indicate that PCR methodology is inaccurate to characterize the prevalence of the cdt genes, since negative PCR detection can be the result of divergences in the sequence used for primer design rather than indicating the absence of a specific gene.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=13033
http://hdl.handle.net/2445/194059
url https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=13033
http://hdl.handle.net/2445/194059
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv WILEY
publisher.none.fl_str_mv WILEY
dc.source.none.fl_str_mv Zoonoses and Public Health
ISSN: 18631959
ISSNe: 18632378
reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
instname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
instname_str Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
reponame_str r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
collection r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
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