First Detection of Methicillin-Resistant Staphylococcus aureus ST398 and Staphylococcus pseudintermedius ST68 from Hospitalized Equines in Spain

Eight coagulase-positive staphylococci from equines with different pathologies obtained between 2005 and 2011 were investigated. Isolates were characterized by different molecular techniques (spa-, agr-, MLST), and clonal relatedness of strains was investigated by ApaI and SmaI PFGE. Anti-microbial...

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Autores: Gómez-Sanz, E. [0000-0001-7666-3642], Simón, C. [0000-0002-4180-1539], Ortega, C. [0000-0002-1210-286X], Gómez, P. [0000-0001-6675-185X], Lozano, C. [0000-0002-0048-9315], Zarazaga, M. [0000-0002-8703-5489], Torres, C. [0000-0003-3709-1690]
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Recursos:Universidad de La Rioja (UR)
Repositorio:RIUR. Repositorio Institucional de la Universidad de La Rioja
OAI Identifier:oai:portal.dialnet.es:doc/5bbc69a3b750603269e81ecc
Acesso em linha:https://investigacion.unirioja.es/documentos/5bbc69a3b750603269e81ecc
Access Level:acceso abierto
Palavra-chave:Equidaes
mecA-positive S. pseudintermedius ST68
MRSA ST398
Spain
Tn559
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network_name_str España
repository_id_str
spelling First Detection of Methicillin-Resistant Staphylococcus aureus ST398 and Staphylococcus pseudintermedius ST68 from Hospitalized Equines in SpainGómez-Sanz, E. [0000-0001-7666-3642]Simón, C. [0000-0002-4180-1539]Ortega, C. [0000-0002-1210-286X]Gómez, P. [0000-0001-6675-185X]Lozano, C. [0000-0002-0048-9315]Zarazaga, M. [0000-0002-8703-5489]Torres, C. [0000-0003-3709-1690]EquidaesmecA-positive S. pseudintermedius ST68MRSA ST398SpainTn559Eight coagulase-positive staphylococci from equines with different pathologies obtained between 2005 and 2011 were investigated. Isolates were characterized by different molecular techniques (spa-, agr-, MLST), and clonal relatedness of strains was investigated by ApaI and SmaI PFGE. Anti-microbial resistance and virulence profiles were determined. Six isolates were identified as Staphylococcus aureus, and two as Staphylococcus pseudintermedius. Of these, four isolates were methicillin-resistant S. aureus (MRSA) ST398 and one S. pseudintermedius was mecA positive and typed as ST68. One MRSA ST398 strain was isolated in 2005 and might be one of the earliest MRSA ST398 descriptions in Spain. All 5 mecA-positive strains were multidrug resistant and were isolated from hospitalized equines. Three MRSA ST398 strains carried the recently described transposon Tn559 within the chromosomal radC gene. The mecA-positive S. pseudintermedius ST68 strain was also multidrug resistant and harboured the erm(B)-Tn5405-like element. This ST68 strain presented a clear susceptible phenotype to oxacillin and cefoxitin regardless of the presence of an integral and conserved mecA gene and mecA promoter, which enhances the need for testing the presence of this gene in routine analysis to avoid treatment failures. These data reflect the extended anti-microbial resistance gene acquisition capacities of both bacterial species and evidence their pathogenic properties. The first detection of MRSA ST398 and S. pseudintermedius ST68 in horses in Spain is reported. © 2013 Blackwell Verlag GmbH.2014info:eu-repo/semantics/articleSubtype: Articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://investigacion.unirioja.es/documentos/5bbc69a3b750603269e81eccreponame:RIUR. Repositorio Institucional de la Universidad de La Riojainstname:Universidad de La Rioja (UR)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1111/ZPH.12059info:eu-repo/semantics/altIdentifier/wos/WOS:000334537200005info:eu-repo/semantics/altIdentifier/pissn/1863-1959First Detection of Methicillin-Resistant Staphylococcus aureus ST398 and Staphylococcus pseudintermedius ST68 from Hospitalized Equines in Spain, 2014, vol. 61, núm. 3, pág. 192-201info:eu-repo/semantics/openAccessoai:portal.dialnet.es:doc/5bbc69a3b750603269e81ecc2026-06-14T12:47:17Z
dc.title.none.fl_str_mv First Detection of Methicillin-Resistant Staphylococcus aureus ST398 and Staphylococcus pseudintermedius ST68 from Hospitalized Equines in Spain
title First Detection of Methicillin-Resistant Staphylococcus aureus ST398 and Staphylococcus pseudintermedius ST68 from Hospitalized Equines in Spain
spellingShingle First Detection of Methicillin-Resistant Staphylococcus aureus ST398 and Staphylococcus pseudintermedius ST68 from Hospitalized Equines in Spain
Gómez-Sanz, E. [0000-0001-7666-3642]
Equidaes
mecA-positive S. pseudintermedius ST68
MRSA ST398
Spain
Tn559
title_short First Detection of Methicillin-Resistant Staphylococcus aureus ST398 and Staphylococcus pseudintermedius ST68 from Hospitalized Equines in Spain
title_full First Detection of Methicillin-Resistant Staphylococcus aureus ST398 and Staphylococcus pseudintermedius ST68 from Hospitalized Equines in Spain
title_fullStr First Detection of Methicillin-Resistant Staphylococcus aureus ST398 and Staphylococcus pseudintermedius ST68 from Hospitalized Equines in Spain
title_full_unstemmed First Detection of Methicillin-Resistant Staphylococcus aureus ST398 and Staphylococcus pseudintermedius ST68 from Hospitalized Equines in Spain
title_sort First Detection of Methicillin-Resistant Staphylococcus aureus ST398 and Staphylococcus pseudintermedius ST68 from Hospitalized Equines in Spain
dc.creator.none.fl_str_mv Gómez-Sanz, E. [0000-0001-7666-3642]
Simón, C. [0000-0002-4180-1539]
Ortega, C. [0000-0002-1210-286X]
Gómez, P. [0000-0001-6675-185X]
Lozano, C. [0000-0002-0048-9315]
Zarazaga, M. [0000-0002-8703-5489]
Torres, C. [0000-0003-3709-1690]
author Gómez-Sanz, E. [0000-0001-7666-3642]
author_facet Gómez-Sanz, E. [0000-0001-7666-3642]
Simón, C. [0000-0002-4180-1539]
Ortega, C. [0000-0002-1210-286X]
Gómez, P. [0000-0001-6675-185X]
Lozano, C. [0000-0002-0048-9315]
Zarazaga, M. [0000-0002-8703-5489]
Torres, C. [0000-0003-3709-1690]
author_role author
author2 Simón, C. [0000-0002-4180-1539]
Ortega, C. [0000-0002-1210-286X]
Gómez, P. [0000-0001-6675-185X]
Lozano, C. [0000-0002-0048-9315]
Zarazaga, M. [0000-0002-8703-5489]
Torres, C. [0000-0003-3709-1690]
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Equidaes
mecA-positive S. pseudintermedius ST68
MRSA ST398
Spain
Tn559
topic Equidaes
mecA-positive S. pseudintermedius ST68
MRSA ST398
Spain
Tn559
description Eight coagulase-positive staphylococci from equines with different pathologies obtained between 2005 and 2011 were investigated. Isolates were characterized by different molecular techniques (spa-, agr-, MLST), and clonal relatedness of strains was investigated by ApaI and SmaI PFGE. Anti-microbial resistance and virulence profiles were determined. Six isolates were identified as Staphylococcus aureus, and two as Staphylococcus pseudintermedius. Of these, four isolates were methicillin-resistant S. aureus (MRSA) ST398 and one S. pseudintermedius was mecA positive and typed as ST68. One MRSA ST398 strain was isolated in 2005 and might be one of the earliest MRSA ST398 descriptions in Spain. All 5 mecA-positive strains were multidrug resistant and were isolated from hospitalized equines. Three MRSA ST398 strains carried the recently described transposon Tn559 within the chromosomal radC gene. The mecA-positive S. pseudintermedius ST68 strain was also multidrug resistant and harboured the erm(B)-Tn5405-like element. This ST68 strain presented a clear susceptible phenotype to oxacillin and cefoxitin regardless of the presence of an integral and conserved mecA gene and mecA promoter, which enhances the need for testing the presence of this gene in routine analysis to avoid treatment failures. These data reflect the extended anti-microbial resistance gene acquisition capacities of both bacterial species and evidence their pathogenic properties. The first detection of MRSA ST398 and S. pseudintermedius ST68 in horses in Spain is reported. © 2013 Blackwell Verlag GmbH.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
Subtype: Article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://investigacion.unirioja.es/documentos/5bbc69a3b750603269e81ecc
url https://investigacion.unirioja.es/documentos/5bbc69a3b750603269e81ecc
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/ZPH.12059
info:eu-repo/semantics/altIdentifier/wos/WOS:000334537200005
info:eu-repo/semantics/altIdentifier/pissn/1863-1959
First Detection of Methicillin-Resistant Staphylococcus aureus ST398 and Staphylococcus pseudintermedius ST68 from Hospitalized Equines in Spain, 2014, vol. 61, núm. 3, pág. 192-201
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIUR. Repositorio Institucional de la Universidad de La Rioja
instname:Universidad de La Rioja (UR)
instname_str Universidad de La Rioja (UR)
reponame_str RIUR. Repositorio Institucional de la Universidad de La Rioja
collection RIUR. Repositorio Institucional de la Universidad de La Rioja
repository.name.fl_str_mv
repository.mail.fl_str_mv
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