Structure-Guided Immobilization of an Evolved Unspecific Peroxygenase

This article belongs to the Special Issue Industrial Enzymes: Structure, Function and Applications.

Detalles Bibliográficos
Autores: Molina-Espeja, Patricia, Santos-Moriano, Paloma, García-Ruiz, Eva, Ballesteros Olmo, Antonio, Plou Gasca, Francisco José, Alcalde Galeote, Miguel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/179141
Acceso en línea:http://hdl.handle.net/10261/179141
Access Level:acceso abierto
Palabra clave:Directed evolution
Structure-guided immobilization
Oxyfunctionalization
Unspecific peroxygenase
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spelling Structure-Guided Immobilization of an Evolved Unspecific PeroxygenaseMolina-Espeja, PatriciaSantos-Moriano, PalomaGarcía-Ruiz, EvaBallesteros Olmo, AntonioPlou Gasca, Francisco JoséAlcalde Galeote, MiguelDirected evolutionStructure-guided immobilizationOxyfunctionalizationUnspecific peroxygenaseThis article belongs to the Special Issue Industrial Enzymes: Structure, Function and Applications.Unspecific peroxygenases (UPOs) are highly promiscuous biocatalyst with self-sufficient mono(per)oxygenase activity. A laboratory-evolved UPO secreted by yeast was covalently immobilized in activated carriers through one-point attachment. In order to maintain the desired orientation without compromising the enzyme’s activity, the S221C mutation was introduced at the surface of the enzyme, enabling a single disulfide bridge to be established between the support and the protein. Fluorescence confocal microscopy demonstrated the homogeneous distribution of the enzyme, regardless of the chemical nature of the carrier. This immobilized biocatalyst was characterized biochemically opening an exciting avenue for research into applied synthetic chemistry.This study is based upon work funded by Repsol S.A and the EU project H2020-BBI-PPP-2015-2-720297-ENZOX2.Peer reviewedMultidisciplinary Digital Publishing InstituteEuropean CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/179141reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/720297https://doi.org/10.3390/ijms20071627Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1791412026-05-22T06:33:51Z
dc.title.none.fl_str_mv Structure-Guided Immobilization of an Evolved Unspecific Peroxygenase
title Structure-Guided Immobilization of an Evolved Unspecific Peroxygenase
spellingShingle Structure-Guided Immobilization of an Evolved Unspecific Peroxygenase
Molina-Espeja, Patricia
Directed evolution
Structure-guided immobilization
Oxyfunctionalization
Unspecific peroxygenase
title_short Structure-Guided Immobilization of an Evolved Unspecific Peroxygenase
title_full Structure-Guided Immobilization of an Evolved Unspecific Peroxygenase
title_fullStr Structure-Guided Immobilization of an Evolved Unspecific Peroxygenase
title_full_unstemmed Structure-Guided Immobilization of an Evolved Unspecific Peroxygenase
title_sort Structure-Guided Immobilization of an Evolved Unspecific Peroxygenase
dc.creator.none.fl_str_mv Molina-Espeja, Patricia
Santos-Moriano, Paloma
García-Ruiz, Eva
Ballesteros Olmo, Antonio
Plou Gasca, Francisco José
Alcalde Galeote, Miguel
author Molina-Espeja, Patricia
author_facet Molina-Espeja, Patricia
Santos-Moriano, Paloma
García-Ruiz, Eva
Ballesteros Olmo, Antonio
Plou Gasca, Francisco José
Alcalde Galeote, Miguel
author_role author
author2 Santos-Moriano, Paloma
García-Ruiz, Eva
Ballesteros Olmo, Antonio
Plou Gasca, Francisco José
Alcalde Galeote, Miguel
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Directed evolution
Structure-guided immobilization
Oxyfunctionalization
Unspecific peroxygenase
topic Directed evolution
Structure-guided immobilization
Oxyfunctionalization
Unspecific peroxygenase
description This article belongs to the Special Issue Industrial Enzymes: Structure, Function and Applications.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/179141
url http://hdl.handle.net/10261/179141
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/720297
https://doi.org/10.3390/ijms20071627
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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