Preclinical Evaluation of the Efficacy of Antivenoms for Snakebite Envenoming: State-of-the-Art and Challenges Ahead.

22 páginas, 3 figuras.

Detalles Bibliográficos
Autores: Gutiérrez, José María, Solano, Gabriela, Pla, Davinia, Herrera, María, Segura, Álvaro, Vargas, Mariángela, Villalta, Mauren, Sánchez, Andrés, Sanz, Libia, Lomonte, Bruno, León, Guillermo, Calvete, Juan J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/151351
Acceso en línea:http://hdl.handle.net/10261/151351
Access Level:acceso abierto
Palabra clave:Antivenomics
Antivenoms
Neutralization tests
Preclinical efficacy
Snake venoms
The 3Rs
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spelling Preclinical Evaluation of the Efficacy of Antivenoms for Snakebite Envenoming: State-of-the-Art and Challenges Ahead.Gutiérrez, José MaríaSolano, GabrielaPla, DaviniaHerrera, MaríaSegura, ÁlvaroVargas, MariángelaVillalta, MaurenSánchez, AndrésSanz, LibiaLomonte, BrunoLeón, GuillermoCalvete, Juan J.AntivenomicsAntivenomsNeutralization testsPreclinical efficacySnake venomsThe 3Rs22 páginas, 3 figuras.Animal-derived antivenoms constitute the mainstay in the therapy of snakebite envenoming. The efficacy of antivenoms to neutralize toxicity of medically-relevant snake venoms has to be demonstrated through meticulous preclinical testing before their introduction into the clinical setting. The gold standard in the preclinical assessment and quality control of antivenoms is the neutralization of venom-induced lethality. In addition, depending on the pathophysiological profile of snake venoms, the neutralization of other toxic activities has to be evaluated, such as hemorrhagic, myotoxic, edema-forming, dermonecrotic, in vitro coagulant, and defibrinogenating effects. There is a need to develop laboratory assays to evaluate neutralization of other relevant venom activities. The concept of the 3Rs (Replacement, Reduction, and Refinement) in Toxinology is of utmost importance, and some advances have been performed in their implementation. A significant leap forward in the study of the immunological reactivity of antivenoms against venoms has been the development of "antivenomics", which brings the analytical power of mass spectrometry to the evaluation of antivenoms. International partnerships are required to assess the preclinical efficacy of antivenoms against snake venoms in different regions of the world in order to have a detailed knowledge on the neutralizing profile of these immunotherapeutics.Many of the studies reviewed in this work have been supported by Vicerrectoría de Investigación (Universidad de Costa Rica), by the program CYTED, and by Grant BFU2013-42833-P from the Ministerio de Economía y Competitividad, Madrid (Spain). The participation of many colleagues and students, in Costa Rica and abroad, in projects dealing with the preclinical efficacy of antivenoms is greatly acknowledged.We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI)Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201720172017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/151351reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#MINECO/BFU2013/42833-PHttp://dx.doi.org/10.3390/toxins9050163Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1513512026-05-22T06:33:51Z
dc.title.none.fl_str_mv Preclinical Evaluation of the Efficacy of Antivenoms for Snakebite Envenoming: State-of-the-Art and Challenges Ahead.
title Preclinical Evaluation of the Efficacy of Antivenoms for Snakebite Envenoming: State-of-the-Art and Challenges Ahead.
spellingShingle Preclinical Evaluation of the Efficacy of Antivenoms for Snakebite Envenoming: State-of-the-Art and Challenges Ahead.
Gutiérrez, José María
Antivenomics
Antivenoms
Neutralization tests
Preclinical efficacy
Snake venoms
The 3Rs
title_short Preclinical Evaluation of the Efficacy of Antivenoms for Snakebite Envenoming: State-of-the-Art and Challenges Ahead.
title_full Preclinical Evaluation of the Efficacy of Antivenoms for Snakebite Envenoming: State-of-the-Art and Challenges Ahead.
title_fullStr Preclinical Evaluation of the Efficacy of Antivenoms for Snakebite Envenoming: State-of-the-Art and Challenges Ahead.
title_full_unstemmed Preclinical Evaluation of the Efficacy of Antivenoms for Snakebite Envenoming: State-of-the-Art and Challenges Ahead.
title_sort Preclinical Evaluation of the Efficacy of Antivenoms for Snakebite Envenoming: State-of-the-Art and Challenges Ahead.
dc.creator.none.fl_str_mv Gutiérrez, José María
Solano, Gabriela
Pla, Davinia
Herrera, María
Segura, Álvaro
Vargas, Mariángela
Villalta, Mauren
Sánchez, Andrés
Sanz, Libia
Lomonte, Bruno
León, Guillermo
Calvete, Juan J.
author Gutiérrez, José María
author_facet Gutiérrez, José María
Solano, Gabriela
Pla, Davinia
Herrera, María
Segura, Álvaro
Vargas, Mariángela
Villalta, Mauren
Sánchez, Andrés
Sanz, Libia
Lomonte, Bruno
León, Guillermo
Calvete, Juan J.
author_role author
author2 Solano, Gabriela
Pla, Davinia
Herrera, María
Segura, Álvaro
Vargas, Mariángela
Villalta, Mauren
Sánchez, Andrés
Sanz, Libia
Lomonte, Bruno
León, Guillermo
Calvete, Juan J.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Antivenomics
Antivenoms
Neutralization tests
Preclinical efficacy
Snake venoms
The 3Rs
topic Antivenomics
Antivenoms
Neutralization tests
Preclinical efficacy
Snake venoms
The 3Rs
description 22 páginas, 3 figuras.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/151351
url http://hdl.handle.net/10261/151351
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
MINECO/BFU2013/42833-P
Http://dx.doi.org/10.3390/toxins9050163
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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