Lipidomics reveals altered biosynthetic pathways of glycerophospholipids and cell signaling as biomarkers of the polycystic ovary syndrome

Purpose: In this work, a non-targeted approach was used to unravel changes in the plasma lipidome of PCOS patients. The aim is to offer new insights in PCOS patients strictly selected in order to avoid confounding factors such as dyslipemia, obesity, altered glucose/insulin metabolism, cardiovascula...

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Autores: Jové Font, Mariona, Pradas Barriga, Irene, Naudí i Farré, Alba, Rovira-Llopis, Susana, Bañuls, Celia, Rocha, Milagros, Portero Otín, Manuel, Hernández-Mijares, Antonio, Victor, Victor M., Pamplona Gras, Reinald
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/62603
Acceso en línea:https://doi.org/10.18632/oncotarget.23393
http://hdl.handle.net/10459.1/62603
Access Level:acceso abierto
Palabra clave:Cell signaling molecules
Glycerophospholipids
Free fatty acids
Lipidomics
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spelling Lipidomics reveals altered biosynthetic pathways of glycerophospholipids and cell signaling as biomarkers of the polycystic ovary syndromeJové Font, MarionaPradas Barriga, IreneNaudí i Farré, AlbaRovira-Llopis, SusanaBañuls, CeliaRocha, MilagrosPortero Otín, ManuelHernández-Mijares, AntonioVictor, Victor M.Pamplona Gras, ReinaldCell signaling moleculesGlycerophospholipidsFree fatty acidsLipidomicsPurpose: In this work, a non-targeted approach was used to unravel changes in the plasma lipidome of PCOS patients. The aim is to offer new insights in PCOS patients strictly selected in order to avoid confounding factors such as dyslipemia, obesity, altered glucose/insulin metabolism, cardiovascular disease, or cancer. Results: Multivariate statistics revealed a specific lipidomic signature for PCOS patients without associated pathologies. This signature implies changes, mainly by down-regulation, in glycerolipid, glycerophospholipid and sphingolipid metabolism suggesting an altered biosynthetic pathway of glycerophospholipids and cell signaling as second messengers in women with PCOS. Conclusions: Our study confirms that a lipidomic approach discriminates a specific phenotype from PCOS women without associated pathologies from healthy controls. Methods: In a cross-sectional pilot study, data were obtained from 34 subjects, allocated to one of two groups: a) lean, healthy controls (n = 20), b) PCOS patients (n = 14) with diagnosis based on hyperandrogenaemia, oligo-anovulation and abnormal ovaries with small follicular cysts. A detailed biochemical characterization was made and lipidomic profiling was performed via an untargeted approach using LC-ESI-QTOF MS/MS.We acknowledge funding from the Fund for Health Research (FIS) and co-funding from the European Regional Development Fund of the European Union (FEDER, ‘Una manera de hacer Europa’): PI15/1424, PI16/1083, PI16/0301 and UGP15-193 by FISABIO, to A.H.M, M.R. and V.M.V, respectively. This research was also in part funded by the Spanish Ministry of Economy and Competitiveness, Institute of Health Carlos III (FIS grants PI14/00328), and the Autonomous Government of Catalonia (2017SGR and SLT002/16/00250) to R.P. This study was co-financed by FEDER funds from the European Union (‘Una manera de hacer Europa’).Impact Journals2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.18632/oncotarget.23393http://hdl.handle.net/10459.1/62603reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a https://doi.org/10.18632/oncotarget.23393Oncotarget, 2017, vol. 9, núm. 4, p. 4522-4536cc-by (c) Jové et al., 2017info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:recercat.cat:10459.1/626032026-05-29T05:05:01Z
dc.title.none.fl_str_mv Lipidomics reveals altered biosynthetic pathways of glycerophospholipids and cell signaling as biomarkers of the polycystic ovary syndrome
title Lipidomics reveals altered biosynthetic pathways of glycerophospholipids and cell signaling as biomarkers of the polycystic ovary syndrome
spellingShingle Lipidomics reveals altered biosynthetic pathways of glycerophospholipids and cell signaling as biomarkers of the polycystic ovary syndrome
Jové Font, Mariona
Cell signaling molecules
Glycerophospholipids
Free fatty acids
Lipidomics
title_short Lipidomics reveals altered biosynthetic pathways of glycerophospholipids and cell signaling as biomarkers of the polycystic ovary syndrome
title_full Lipidomics reveals altered biosynthetic pathways of glycerophospholipids and cell signaling as biomarkers of the polycystic ovary syndrome
title_fullStr Lipidomics reveals altered biosynthetic pathways of glycerophospholipids and cell signaling as biomarkers of the polycystic ovary syndrome
title_full_unstemmed Lipidomics reveals altered biosynthetic pathways of glycerophospholipids and cell signaling as biomarkers of the polycystic ovary syndrome
title_sort Lipidomics reveals altered biosynthetic pathways of glycerophospholipids and cell signaling as biomarkers of the polycystic ovary syndrome
dc.creator.none.fl_str_mv Jové Font, Mariona
Pradas Barriga, Irene
Naudí i Farré, Alba
Rovira-Llopis, Susana
Bañuls, Celia
Rocha, Milagros
Portero Otín, Manuel
Hernández-Mijares, Antonio
Victor, Victor M.
Pamplona Gras, Reinald
author Jové Font, Mariona
author_facet Jové Font, Mariona
Pradas Barriga, Irene
Naudí i Farré, Alba
Rovira-Llopis, Susana
Bañuls, Celia
Rocha, Milagros
Portero Otín, Manuel
Hernández-Mijares, Antonio
Victor, Victor M.
Pamplona Gras, Reinald
author_role author
author2 Pradas Barriga, Irene
Naudí i Farré, Alba
Rovira-Llopis, Susana
Bañuls, Celia
Rocha, Milagros
Portero Otín, Manuel
Hernández-Mijares, Antonio
Victor, Victor M.
Pamplona Gras, Reinald
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cell signaling molecules
Glycerophospholipids
Free fatty acids
Lipidomics
topic Cell signaling molecules
Glycerophospholipids
Free fatty acids
Lipidomics
description Purpose: In this work, a non-targeted approach was used to unravel changes in the plasma lipidome of PCOS patients. The aim is to offer new insights in PCOS patients strictly selected in order to avoid confounding factors such as dyslipemia, obesity, altered glucose/insulin metabolism, cardiovascular disease, or cancer. Results: Multivariate statistics revealed a specific lipidomic signature for PCOS patients without associated pathologies. This signature implies changes, mainly by down-regulation, in glycerolipid, glycerophospholipid and sphingolipid metabolism suggesting an altered biosynthetic pathway of glycerophospholipids and cell signaling as second messengers in women with PCOS. Conclusions: Our study confirms that a lipidomic approach discriminates a specific phenotype from PCOS women without associated pathologies from healthy controls. Methods: In a cross-sectional pilot study, data were obtained from 34 subjects, allocated to one of two groups: a) lean, healthy controls (n = 20), b) PCOS patients (n = 14) with diagnosis based on hyperandrogenaemia, oligo-anovulation and abnormal ovaries with small follicular cysts. A detailed biochemical characterization was made and lipidomic profiling was performed via an untargeted approach using LC-ESI-QTOF MS/MS.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.18632/oncotarget.23393
http://hdl.handle.net/10459.1/62603
url https://doi.org/10.18632/oncotarget.23393
http://hdl.handle.net/10459.1/62603
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a https://doi.org/10.18632/oncotarget.23393
Oncotarget, 2017, vol. 9, núm. 4, p. 4522-4536
dc.rights.none.fl_str_mv cc-by (c) Jové et al., 2017
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Jové et al., 2017
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Impact Journals
publisher.none.fl_str_mv Impact Journals
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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