Genomic and phenotypic characterization of 26 novel marine bacterial strains with relevant biogeochemical roles and widespread presence across the global ocean

Prokaryotes dominate global oceans and shape biogeochemical cycles, yet most taxa remain uncultured and uncharacterized as of today. Here we present the characterization of 26 novel marine bacterial strains from a large isolate collection obtained from Blanes Bay (NW Mediterranean) microcosm experim...

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Autores: Rey-Velasco, Xavier|||0000-0002-6970-6234, Lucena, Teresa, Belda, Ana, Gasol, Josep M.|||0000-0001-5238-2387, Sánchez Martínez, M. Olga|||0000-0003-1254-012X, Arahal, David R.|||0000-0002-8926-9801, Pujalte, María J.|||0000-0001-6048-9723
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:312384
Acceso en línea:https://ddd.uab.cat/record/312384
https://dx.doi.org/urn:doi:10.3389/fmicb.2024.1407904
Access Level:acceso abierto
Palabra clave:Taxonomy
Genomics
Phylogenomics
Isolates
Marine bacteria
Biogeochemistry
Biogeography
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spelling Genomic and phenotypic characterization of 26 novel marine bacterial strains with relevant biogeochemical roles and widespread presence across the global oceanRey-Velasco, Xavier|||0000-0002-6970-6234Lucena, TeresaBelda, AnaGasol, Josep M.|||0000-0001-5238-2387Sánchez Martínez, M. Olga|||0000-0003-1254-012XArahal, David R.|||0000-0002-8926-9801Pujalte, María J.|||0000-0001-6048-9723TaxonomyGenomicsPhylogenomicsIsolatesMarine bacteriaBiogeochemistryBiogeographyProkaryotes dominate global oceans and shape biogeochemical cycles, yet most taxa remain uncultured and uncharacterized as of today. Here we present the characterization of 26 novel marine bacterial strains from a large isolate collection obtained from Blanes Bay (NW Mediterranean) microcosm experiments made in the four seasons. Morphological, cultural, biochemical, physiological, nutritional, genomic, and phylogenomic analyses were used to characterize and phylogenetically place the novel isolates. The strains represent 23 novel bacterial species and six novel genera: three novel species pertaining to class Alphaproteobacteria (families Rhodobacteraceae and Sphingomonadaceae), six novel species and three new genera from class Gammaproteobacteria (families Algiphilaceae, Salinispheraceae, and Alteromonadaceae), 13 novel species and three novel genera from class Bacteroidia (family Flavobacteriaceae), and one new species from class Rhodothermia (family Rubricoccaceae). The bacteria described here have potentially relevant roles in the cycles of carbon (e.g., carbon fixation or energy production via proteorhodopsin), nitrogen (e.g., denitrification or use of urea), sulfur (oxidation of sulfur compounds), phosphorus (acquisition and use of different forms of phosphorus and remodeling of membrane phospholipids), and hydrogen (oxidation of hydrogen to obtain energy). We mapped the genomes of the presented strains to the Tara Oceans metagenomes to reveal that these strains were globally distributed, with those of the family Flavobacteriaceae being the most widespread and abundant, while Rhodothermia being the rarest and most localized. While molecular-only approaches are also important, our study stresses the importance of culturing as a powerful tool to further understand the functioning of marine bacterial communities. 22024-01-0120242024-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/312384https://dx.doi.org/urn:doi:10.3389/fmicb.2024.1407904reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-101025-B-I00Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2021-125469NB-C31Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2021-125469NB-C32Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 CEX2019-000928-Sopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3123842026-06-06T12:50:31Z
dc.title.none.fl_str_mv Genomic and phenotypic characterization of 26 novel marine bacterial strains with relevant biogeochemical roles and widespread presence across the global ocean
title Genomic and phenotypic characterization of 26 novel marine bacterial strains with relevant biogeochemical roles and widespread presence across the global ocean
spellingShingle Genomic and phenotypic characterization of 26 novel marine bacterial strains with relevant biogeochemical roles and widespread presence across the global ocean
Rey-Velasco, Xavier|||0000-0002-6970-6234
Taxonomy
Genomics
Phylogenomics
Isolates
Marine bacteria
Biogeochemistry
Biogeography
title_short Genomic and phenotypic characterization of 26 novel marine bacterial strains with relevant biogeochemical roles and widespread presence across the global ocean
title_full Genomic and phenotypic characterization of 26 novel marine bacterial strains with relevant biogeochemical roles and widespread presence across the global ocean
title_fullStr Genomic and phenotypic characterization of 26 novel marine bacterial strains with relevant biogeochemical roles and widespread presence across the global ocean
title_full_unstemmed Genomic and phenotypic characterization of 26 novel marine bacterial strains with relevant biogeochemical roles and widespread presence across the global ocean
title_sort Genomic and phenotypic characterization of 26 novel marine bacterial strains with relevant biogeochemical roles and widespread presence across the global ocean
dc.creator.none.fl_str_mv Rey-Velasco, Xavier|||0000-0002-6970-6234
Lucena, Teresa
Belda, Ana
Gasol, Josep M.|||0000-0001-5238-2387
Sánchez Martínez, M. Olga|||0000-0003-1254-012X
Arahal, David R.|||0000-0002-8926-9801
Pujalte, María J.|||0000-0001-6048-9723
author Rey-Velasco, Xavier|||0000-0002-6970-6234
author_facet Rey-Velasco, Xavier|||0000-0002-6970-6234
Lucena, Teresa
Belda, Ana
Gasol, Josep M.|||0000-0001-5238-2387
Sánchez Martínez, M. Olga|||0000-0003-1254-012X
Arahal, David R.|||0000-0002-8926-9801
Pujalte, María J.|||0000-0001-6048-9723
author_role author
author2 Lucena, Teresa
Belda, Ana
Gasol, Josep M.|||0000-0001-5238-2387
Sánchez Martínez, M. Olga|||0000-0003-1254-012X
Arahal, David R.|||0000-0002-8926-9801
Pujalte, María J.|||0000-0001-6048-9723
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Taxonomy
Genomics
Phylogenomics
Isolates
Marine bacteria
Biogeochemistry
Biogeography
topic Taxonomy
Genomics
Phylogenomics
Isolates
Marine bacteria
Biogeochemistry
Biogeography
description Prokaryotes dominate global oceans and shape biogeochemical cycles, yet most taxa remain uncultured and uncharacterized as of today. Here we present the characterization of 26 novel marine bacterial strains from a large isolate collection obtained from Blanes Bay (NW Mediterranean) microcosm experiments made in the four seasons. Morphological, cultural, biochemical, physiological, nutritional, genomic, and phylogenomic analyses were used to characterize and phylogenetically place the novel isolates. The strains represent 23 novel bacterial species and six novel genera: three novel species pertaining to class Alphaproteobacteria (families Rhodobacteraceae and Sphingomonadaceae), six novel species and three new genera from class Gammaproteobacteria (families Algiphilaceae, Salinispheraceae, and Alteromonadaceae), 13 novel species and three novel genera from class Bacteroidia (family Flavobacteriaceae), and one new species from class Rhodothermia (family Rubricoccaceae). The bacteria described here have potentially relevant roles in the cycles of carbon (e.g., carbon fixation or energy production via proteorhodopsin), nitrogen (e.g., denitrification or use of urea), sulfur (oxidation of sulfur compounds), phosphorus (acquisition and use of different forms of phosphorus and remodeling of membrane phospholipids), and hydrogen (oxidation of hydrogen to obtain energy). We mapped the genomes of the presented strains to the Tara Oceans metagenomes to reveal that these strains were globally distributed, with those of the family Flavobacteriaceae being the most widespread and abundant, while Rhodothermia being the rarest and most localized. While molecular-only approaches are also important, our study stresses the importance of culturing as a powerful tool to further understand the functioning of marine bacterial communities.
publishDate 2024
dc.date.none.fl_str_mv 2
2024-01-01
2024
2024-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/312384
https://dx.doi.org/urn:doi:10.3389/fmicb.2024.1407904
url https://ddd.uab.cat/record/312384
https://dx.doi.org/urn:doi:10.3389/fmicb.2024.1407904
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-101025-B-I00
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2021-125469NB-C31
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2021-125469NB-C32
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 CEX2019-000928-S
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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