Effect of Extender, Storage Time and Temperature on Kinetic Parameters (CASA) on Bull Semen Samples

CASA kinetic parameters are often evaluated in a diagnostic centre. How storage conditions affect ejaculates up to evaluation is unclear. We assessed, in 25 commercial bulls electroejaculated in the field, the impact of time until evaluation (0–2 h, 4–6 h, and 24 h post-ejaculation), holding tempera...

Descripción completa

Detalles Bibliográficos
Autores: Fernández Novo, Aitor, Santos López, Sergio, Barrajón Masa, Clara, Mozas, Patricia, Mercado, Eduardo de, Cáceres, Elisa, Garrafa, Aizic, González Martín, Juan Vicente, Pérez Villalobos, Natividad, Oliet, Agustín, Astiz Blanco, Susana, Pérez Garnelo, Sonia S.
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Europea (UEM)
Repositorio:ABACUS. Repositorio de Producción Científica
Idioma:inglés
OAI Identifier:oai:abacus.universidadeuropea.com:11268/10411
Acceso en línea:http://hdl.handle.net/11268/10411
Access Level:acceso abierto
Palabra clave:Fertilización
Semen
Medicina veterinaria
Biología
Fecundidad
Ganado vacuno
Descripción
Sumario:CASA kinetic parameters are often evaluated in a diagnostic centre. How storage conditions affect ejaculates up to evaluation is unclear. We assessed, in 25 commercial bulls electroejaculated in the field, the impact of time until evaluation (0–2 h, 4–6 h, and 24 h post-ejaculation), holding temperature (5 °C vs. room temperature), and extender (AndroMed®, BIOXcell® or INRA96®) on CASA kinetic parameters. Total and progressive motility, VCL, VAP, VCL, ALH, BCF, STR, LIN, and WOB were assessed. CASA kinetic parameters were preserved for up to 4–6 h post-ejaculation, except for AndroMed®. Regardless of extender or temperature, motility decreased from 4–6 h up to 24 h, with the best values obtained with BIOXcell® at 5 °C. Our results suggest that BIOXcell® can preserve sperm motility for up to 6 h, either at 5 °C or room temperature, and also INRA96® at room temperature, with motility assessments and the percentage of the most rapid sperms being the lowest with INRA96® at 5 °C. The kinetic parameters decreased when analyses were performed at 24 h. Therefore, we suggest evaluating seminal quality as soon as possible, before 6 h after collection. These results help to fix adequate protocols for the short-term storage and shipment of bovine semen collected under field conditions.

Similares en LA Referencia