RAD51 as a biomarker for homologous recombination deficiency in high-grade serous ovarian carcinoma: robustness and interobserver variability of the RAD51 test

Biomarker; High-hrade serous ovarian carcinoma; Homologous recombination deficiency

Detalhes bibliográficos
Autores: Kramer, Claire, Yaniz-Galende, Elisa, Pellegrino, Benedetta, ter Haar, Natalja, Llop-Guevara, Alba, Herencia Ropero, Andrea, Serra, Violeta
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:11351/10446
Acesso em linha:https://hdl.handle.net/11351/10446
http://hdl.handle.net/11351/10446
Access Level:acceso abierto
Palavra-chave:Ovaris - Càncer - Aspectes genètics
Marcadors tumorals
Recombinació genètica
DISEASES::Neoplasms::Neoplasms by Site::Endocrine Gland Neoplasms::Ovarian Neoplasms
PHENOMENA AND PROCESSES::Genetic Phenomena::Recombination, Genetic::Homologous Recombination
CHEMICALS AND DRUGS::Biological Factors::Biomarkers::Biomarkers, Tumor
CHEMICALS AND DRUGS::Enzymes and Coenzymes::Enzymes::Recombinases::Rec A Recombinases::Rad51 Recombinase
ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias de las glándulas endocrinas::neoplasias ováricas
FENÓMENOS Y PROCESOS::fenómenos genéticos::recombinación genética::recombinación homóloga
COMPUESTOS QUÍMICOS Y DROGAS::factores biológicos::biomarcadores::marcadores tumorales
COMPUESTOS QUÍMICOS Y DROGAS::enzimas y coenzimas::enzimas::recombinasas::rec a recombinasas::rad51 recombinasa
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oai_identifier_str oai:recercat.cat:11351/10446
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv RAD51 as a biomarker for homologous recombination deficiency in high-grade serous ovarian carcinoma: robustness and interobserver variability of the RAD51 test
title RAD51 as a biomarker for homologous recombination deficiency in high-grade serous ovarian carcinoma: robustness and interobserver variability of the RAD51 test
spellingShingle RAD51 as a biomarker for homologous recombination deficiency in high-grade serous ovarian carcinoma: robustness and interobserver variability of the RAD51 test
Kramer, Claire
Ovaris - Càncer - Aspectes genètics
Marcadors tumorals
Recombinació genètica
DISEASES::Neoplasms::Neoplasms by Site::Endocrine Gland Neoplasms::Ovarian Neoplasms
PHENOMENA AND PROCESSES::Genetic Phenomena::Recombination, Genetic::Homologous Recombination
CHEMICALS AND DRUGS::Biological Factors::Biomarkers::Biomarkers, Tumor
CHEMICALS AND DRUGS::Enzymes and Coenzymes::Enzymes::Recombinases::Rec A Recombinases::Rad51 Recombinase
ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias de las glándulas endocrinas::neoplasias ováricas
FENÓMENOS Y PROCESOS::fenómenos genéticos::recombinación genética::recombinación homóloga
COMPUESTOS QUÍMICOS Y DROGAS::factores biológicos::biomarcadores::marcadores tumorales
COMPUESTOS QUÍMICOS Y DROGAS::enzimas y coenzimas::enzimas::recombinasas::rec a recombinasas::rad51 recombinasa
title_short RAD51 as a biomarker for homologous recombination deficiency in high-grade serous ovarian carcinoma: robustness and interobserver variability of the RAD51 test
title_full RAD51 as a biomarker for homologous recombination deficiency in high-grade serous ovarian carcinoma: robustness and interobserver variability of the RAD51 test
title_fullStr RAD51 as a biomarker for homologous recombination deficiency in high-grade serous ovarian carcinoma: robustness and interobserver variability of the RAD51 test
title_full_unstemmed RAD51 as a biomarker for homologous recombination deficiency in high-grade serous ovarian carcinoma: robustness and interobserver variability of the RAD51 test
title_sort RAD51 as a biomarker for homologous recombination deficiency in high-grade serous ovarian carcinoma: robustness and interobserver variability of the RAD51 test
dc.creator.none.fl_str_mv Kramer, Claire
Yaniz-Galende, Elisa
Pellegrino, Benedetta
ter Haar, Natalja
Llop-Guevara, Alba
Herencia Ropero, Andrea
Serra, Violeta
author Kramer, Claire
author_facet Kramer, Claire
Yaniz-Galende, Elisa
Pellegrino, Benedetta
ter Haar, Natalja
Llop-Guevara, Alba
Herencia Ropero, Andrea
Serra, Violeta
author_role author
author2 Yaniz-Galende, Elisa
Pellegrino, Benedetta
ter Haar, Natalja
Llop-Guevara, Alba
Herencia Ropero, Andrea
Serra, Violeta
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Institut Català de la Salut
[Kramer CJ, Ter Haar NT] Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands. [Llop-Guevara A, Herencia-Ropero A, Serra V] Grup de Terapèutica Experimental, Vall d'Hebron Institut d'Oncologia (VHIO), Barcelona, Spain. [Yaniz-Galende E] Department of Oncology, Institute Gustave Roussy, Villejuif, France. [Pellegrino B] Department of Medicine and Surgery, University of Parma, Parma, Italy. Medical Oncology and Breast Unit, University Hospital of Parma, Parma, Italy
Vall d'Hebron Barcelona Hospital Campus
dc.subject.none.fl_str_mv Ovaris - Càncer - Aspectes genètics
Marcadors tumorals
Recombinació genètica
DISEASES::Neoplasms::Neoplasms by Site::Endocrine Gland Neoplasms::Ovarian Neoplasms
PHENOMENA AND PROCESSES::Genetic Phenomena::Recombination, Genetic::Homologous Recombination
CHEMICALS AND DRUGS::Biological Factors::Biomarkers::Biomarkers, Tumor
CHEMICALS AND DRUGS::Enzymes and Coenzymes::Enzymes::Recombinases::Rec A Recombinases::Rad51 Recombinase
ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias de las glándulas endocrinas::neoplasias ováricas
FENÓMENOS Y PROCESOS::fenómenos genéticos::recombinación genética::recombinación homóloga
COMPUESTOS QUÍMICOS Y DROGAS::factores biológicos::biomarcadores::marcadores tumorales
COMPUESTOS QUÍMICOS Y DROGAS::enzimas y coenzimas::enzimas::recombinasas::rec a recombinasas::rad51 recombinasa
topic Ovaris - Càncer - Aspectes genètics
Marcadors tumorals
Recombinació genètica
DISEASES::Neoplasms::Neoplasms by Site::Endocrine Gland Neoplasms::Ovarian Neoplasms
PHENOMENA AND PROCESSES::Genetic Phenomena::Recombination, Genetic::Homologous Recombination
CHEMICALS AND DRUGS::Biological Factors::Biomarkers::Biomarkers, Tumor
CHEMICALS AND DRUGS::Enzymes and Coenzymes::Enzymes::Recombinases::Rec A Recombinases::Rad51 Recombinase
ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias de las glándulas endocrinas::neoplasias ováricas
FENÓMENOS Y PROCESOS::fenómenos genéticos::recombinación genética::recombinación homóloga
COMPUESTOS QUÍMICOS Y DROGAS::factores biológicos::biomarcadores::marcadores tumorales
COMPUESTOS QUÍMICOS Y DROGAS::enzimas y coenzimas::enzimas::recombinasas::rec a recombinasas::rad51 recombinasa
description Biomarker; High-hrade serous ovarian carcinoma; Homologous recombination deficiency
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11351/10446
http://hdl.handle.net/11351/10446
url https://hdl.handle.net/11351/10446
http://hdl.handle.net/11351/10446
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The Journal of Pathology: Clinical Research;9(6)
https://doi.org/10.1002/cjp2.336
info:eu-repo/grantAgreement/ES/PE2017-2020/CPII19%2F00033
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Scientia
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405786702086144
spelling RAD51 as a biomarker for homologous recombination deficiency in high-grade serous ovarian carcinoma: robustness and interobserver variability of the RAD51 testKramer, ClaireYaniz-Galende, ElisaPellegrino, Benedettater Haar, NataljaLlop-Guevara, AlbaHerencia Ropero, AndreaSerra, VioletaOvaris - Càncer - Aspectes genèticsMarcadors tumoralsRecombinació genèticaDISEASES::Neoplasms::Neoplasms by Site::Endocrine Gland Neoplasms::Ovarian NeoplasmsPHENOMENA AND PROCESSES::Genetic Phenomena::Recombination, Genetic::Homologous RecombinationCHEMICALS AND DRUGS::Biological Factors::Biomarkers::Biomarkers, TumorCHEMICALS AND DRUGS::Enzymes and Coenzymes::Enzymes::Recombinases::Rec A Recombinases::Rad51 RecombinaseENFERMEDADES::neoplasias::neoplasias por localización::neoplasias de las glándulas endocrinas::neoplasias ováricasFENÓMENOS Y PROCESOS::fenómenos genéticos::recombinación genética::recombinación homólogaCOMPUESTOS QUÍMICOS Y DROGAS::factores biológicos::biomarcadores::marcadores tumoralesCOMPUESTOS QUÍMICOS Y DROGAS::enzimas y coenzimas::enzimas::recombinasas::rec a recombinasas::rad51 recombinasaBiomarker; High-hrade serous ovarian carcinoma; Homologous recombination deficiencyBiomarcador; Carcinoma de ovario seroso de alto grado; Deficiencia de recombinación homólogaBiomarcador; Carcinoma d'ovari serós d'alt grau; Deficiència de recombinació homòlogaThe RAD51 test is emerging as a promising biomarker for the assessment of functional homologous recombination deficiency (HRD). Yet, the robustness and reproducibility of the immunofluorescence-based RAD51 test, in different academic laboratories, have not been systematically investigated. Therefore, we tested the performance of the RAD51 assay in formalin-fixed paraffin-embedded (FFPE) high-grade serous ovarian carcinoma (HGSOC) samples in four European laboratories. Here, we confirm that subtle differences in staining procedures result in low variability of RAD51 and γH2AX scores. However, substantial variability in RAD51 scoring was observed in some samples, likely due to complicating technical and biological features, such as high RAD51 signal-to-noise ratio and RAD51 heterogeneity. These results support the need to identify and perform additional quality control steps and/or automating image analysis. Altogether, resolving technical issues should be a priority, as identifying tumours with functional HRD is urgently needed to guide the individual treatment of HGSOC patients. Follow-up studies are needed to define the key tissue quality requirements to assess HRD by RAD51 in FFPE tumour samples, as this test could help in guiding the individual treatment of HGSOC patients.This work was supported by the Dutch Cancer Society (KWF) (grant: 12995 to TB and MV) and by ERA PerMed (grant ERAPERMED2019-215 to VS). VS received funding from Instituto de Salud Carlos III (CPII19/00033). ALG received funding from Asociación Española Contra el Cáncer (AECC) (INVES20095LLOP) and AHR from Generalitat de Catalunya (PERIS SLT017/20/000081).WileyInstitut Català de la Salut[Kramer CJ, Ter Haar NT] Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands. [Llop-Guevara A, Herencia-Ropero A, Serra V] Grup de Terapèutica Experimental, Vall d'Hebron Institut d'Oncologia (VHIO), Barcelona, Spain. [Yaniz-Galende E] Department of Oncology, Institute Gustave Roussy, Villejuif, France. [Pellegrino B] Department of Medicine and Surgery, University of Parma, Parma, Italy. Medical Oncology and Breast Unit, University Hospital of Parma, Parma, ItalyVall d'Hebron Barcelona Hospital Campus202320232023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11351/10446http://hdl.handle.net/11351/10446Scientiareponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésThe Journal of Pathology: Clinical Research;9(6)https://doi.org/10.1002/cjp2.336info:eu-repo/grantAgreement/ES/PE2017-2020/CPII19%2F00033Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:11351/104462026-05-29T05:05:01Z
score 15,812429

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