Attenuated epigenetic suppression of muscle stem cell necroptosis is required for efficient regeneration of dystrophic muscles

Somatic stem cells expand massively during tissue regeneration, which might require control of cell fitness, allowing elimination of non-competitive, potentially harmful cells. How or if such cells are removed to restore organ function is not fully understood. Here, we show that a substantial fracti...

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Bibliographic Details
Authors: Sreenivasan, Krishnamoorthy, Ianni, Alessandro, Künne, Carsten, Strilic, Boris, Günther, Stefan, Perdiguero, Eusebio, 1968-, Krüger, Marcus, Spuler, Simone, Offermanns, Stefan, Gómez del Arco, Pablo, Redondo, Juan Miguel, Muñoz Cánoves, Pura, 1962-, Kim, Johnny, Braun, Thomas
Format: article
Status:Published version
Publication Date:2020
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/44880
Online Access:http://hdl.handle.net/10230/44880
http://dx.doi.org/10.1016/j.celrep.2020.107652
Access Level:Open access
Keyword:Chd4/NuRD
Ripk3
Muscle dystrophy
Muscle stem cells
Necroptosis
Regeneration
Description
Summary:Somatic stem cells expand massively during tissue regeneration, which might require control of cell fitness, allowing elimination of non-competitive, potentially harmful cells. How or if such cells are removed to restore organ function is not fully understood. Here, we show that a substantial fraction of muscle stem cells (MuSCs) undergo necroptosis because of epigenetic rewiring during chronic skeletal muscle regeneration, which is required for efficient regeneration of dystrophic muscles. Inhibition of necroptosis strongly enhances suppression of MuSC expansion in a non-cell-autonomous manner. Prevention of necroptosis in MuSCs of healthy muscles is mediated by the chromatin remodeler CHD4, which directly represses the necroptotic effector Ripk3, while CHD4-dependent Ripk3 repression is dramatically attenuated in dystrophic muscles. Loss of Ripk3 repression by inactivation of Chd4 causes massive necroptosis of MuSCs, abolishing regeneration. Our study demonstrates how programmed cell death in MuSCs is tightly controlled to achieve optimal tissue regeneration.

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