The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility

[EN]The ubiquitin proteasome system regulates meiotic recombination in yeast through its association with the synaptonemal complex, a ‘zipper’-like structure that holds homologous chromosome pairs in synapsis during meiotic prophase I. In mammals, the proteasome activator subunit PA200 targets acety...

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Autores: Gómez Hernández, Laura, Felipe-Medina, Natalia, Condezo, Yazmine B., García-Valiente, Rodrigo, Ramos, Isabel, Suja, José Angel, Barbero, José Luis, Roig, Ignasi, Sánchez Martín, Manuel Adolfo, de Rooij, Dirk G., Llano Cuadra, María Elena, Martín Pendás, Alberto
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/143907
Acceso en línea:http://hdl.handle.net/10366/143907
Access Level:acceso abierto
Palabra clave:Meiotic prophase
Spermatogenesis
Proteasomes
Protein extraction
Meiotic Prophase I
2415 Biología Molecular
profase meiótica I
espermatogénesis
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oai_identifier_str oai:gredos.usal.es:10366/143907
network_acronym_str ES
network_name_str España
repository_id_str
spelling The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertilityGómez Hernández, LauraFelipe-Medina, NataliaCondezo, Yazmine B.García-Valiente, RodrigoRamos, IsabelSuja, José AngelBarbero, José LuisRoig, IgnasiSánchez Martín, Manuel Adolfode Rooij, Dirk G.Llano Cuadra, María ElenaMartín Pendás, AlbertoMeiotic prophaseSpermatogenesisProteasomesProtein extractionSpermatogenesisMeiotic Prophase I2415 Biología Molecularprofase meiótica Iespermatogénesis[EN]The ubiquitin proteasome system regulates meiotic recombination in yeast through its association with the synaptonemal complex, a ‘zipper’-like structure that holds homologous chromosome pairs in synapsis during meiotic prophase I. In mammals, the proteasome activator subunit PA200 targets acetylated histones for degradation during somatic DNA double strand break repair and during histone replacement during spermiogenesis. We investigated the role of the testis-specific proteasomal subunit α4s (PSMA8) during spermatogenesis, and found that PSMA8 was localized to and dependent on the central region of the synaptonemal complex. Accordingly, synapsis-deficient mice show delocalization of PSMA8. Moreover, though Psma8-deficient mice are proficient in meiotic homologous recombination, there are alterations in the proteostasis of several key meiotic players that, in addition to the known substrate acetylated histones, have been shown by a proteomic approach to interact with PSMA8, such as SYCP3, SYCP1, CDK1 and TRIP13. These alterations lead to an accumulation of spermatocytes in metaphase I and II which either enter massively into apoptosis or give rise to a low number of aberrant round spermatids that apoptose before histone replacement takes place.MINECO ; Junta de Castilla y LeónPublic Library of Science (New York)202020202019info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10366/143907reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésBFU2017-89408-RCSI239P18Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1439072026-06-07T06:28:51Z
dc.title.none.fl_str_mv The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility
title The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility
spellingShingle The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility
Gómez Hernández, Laura
Meiotic prophase
Spermatogenesis
Proteasomes
Protein extraction
Spermatogenesis
Meiotic Prophase I
2415 Biología Molecular
profase meiótica I
espermatogénesis
title_short The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility
title_full The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility
title_fullStr The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility
title_full_unstemmed The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility
title_sort The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility
dc.creator.none.fl_str_mv Gómez Hernández, Laura
Felipe-Medina, Natalia
Condezo, Yazmine B.
García-Valiente, Rodrigo
Ramos, Isabel
Suja, José Angel
Barbero, José Luis
Roig, Ignasi
Sánchez Martín, Manuel Adolfo
de Rooij, Dirk G.
Llano Cuadra, María Elena
Martín Pendás, Alberto
author Gómez Hernández, Laura
author_facet Gómez Hernández, Laura
Felipe-Medina, Natalia
Condezo, Yazmine B.
García-Valiente, Rodrigo
Ramos, Isabel
Suja, José Angel
Barbero, José Luis
Roig, Ignasi
Sánchez Martín, Manuel Adolfo
de Rooij, Dirk G.
Llano Cuadra, María Elena
Martín Pendás, Alberto
author_role author
author2 Felipe-Medina, Natalia
Condezo, Yazmine B.
García-Valiente, Rodrigo
Ramos, Isabel
Suja, José Angel
Barbero, José Luis
Roig, Ignasi
Sánchez Martín, Manuel Adolfo
de Rooij, Dirk G.
Llano Cuadra, María Elena
Martín Pendás, Alberto
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Meiotic prophase
Spermatogenesis
Proteasomes
Protein extraction
Spermatogenesis
Meiotic Prophase I
2415 Biología Molecular
profase meiótica I
espermatogénesis
topic Meiotic prophase
Spermatogenesis
Proteasomes
Protein extraction
Spermatogenesis
Meiotic Prophase I
2415 Biología Molecular
profase meiótica I
espermatogénesis
description [EN]The ubiquitin proteasome system regulates meiotic recombination in yeast through its association with the synaptonemal complex, a ‘zipper’-like structure that holds homologous chromosome pairs in synapsis during meiotic prophase I. In mammals, the proteasome activator subunit PA200 targets acetylated histones for degradation during somatic DNA double strand break repair and during histone replacement during spermiogenesis. We investigated the role of the testis-specific proteasomal subunit α4s (PSMA8) during spermatogenesis, and found that PSMA8 was localized to and dependent on the central region of the synaptonemal complex. Accordingly, synapsis-deficient mice show delocalization of PSMA8. Moreover, though Psma8-deficient mice are proficient in meiotic homologous recombination, there are alterations in the proteostasis of several key meiotic players that, in addition to the known substrate acetylated histones, have been shown by a proteomic approach to interact with PSMA8, such as SYCP3, SYCP1, CDK1 and TRIP13. These alterations lead to an accumulation of spermatocytes in metaphase I and II which either enter massively into apoptosis or give rise to a low number of aberrant round spermatids that apoptose before histone replacement takes place.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/143907
url http://hdl.handle.net/10366/143907
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv BFU2017-89408-R
CSI239P18
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (New York)
publisher.none.fl_str_mv Public Library of Science (New York)
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405782619979776
score 15.300724

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