Ultrastructure of the early stages of Colletotrichum acutatum infection of strawberry tissues
The early stages of the infection of attached leaves and petioles of strawberry (Fragaria ×ananassa Duch. ‘Camarosa’) by Colletotrichum acutatum Simmonds were studied using scanning and transmission electron microscopy. Pre-penetration events of these tissues were similar, but the production of seco...
| Autores: | , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2005 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/40306 |
| Acceso en línea: | http://hdl.handle.net/11441/40306 https://doi.org/10.1139/B05-022 |
| Access Level: | acceso abierto |
| Palabra clave: | Colletotrichum acutatum Fragaria ×ananassa Infection vesicle Invasion strategy Penetration peg |
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oai:idus.us.es:11441/40306 |
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España |
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|
| dc.title.none.fl_str_mv |
Ultrastructure of the early stages of Colletotrichum acutatum infection of strawberry tissues |
| title |
Ultrastructure of the early stages of Colletotrichum acutatum infection of strawberry tissues |
| spellingShingle |
Ultrastructure of the early stages of Colletotrichum acutatum infection of strawberry tissues Arroyo Cordero, Francisco Teodoro Colletotrichum acutatum Fragaria ×ananassa Infection vesicle Invasion strategy Penetration peg |
| title_short |
Ultrastructure of the early stages of Colletotrichum acutatum infection of strawberry tissues |
| title_full |
Ultrastructure of the early stages of Colletotrichum acutatum infection of strawberry tissues |
| title_fullStr |
Ultrastructure of the early stages of Colletotrichum acutatum infection of strawberry tissues |
| title_full_unstemmed |
Ultrastructure of the early stages of Colletotrichum acutatum infection of strawberry tissues |
| title_sort |
Ultrastructure of the early stages of Colletotrichum acutatum infection of strawberry tissues |
| dc.creator.none.fl_str_mv |
Arroyo Cordero, Francisco Teodoro Moreno Onorato, Francisco Javier García Herdugo, Gregorio Santos García de Paredes, Berta de los Barrau García, Carmen Porras, María Blanco Ruiz, César Romero Muñoz, Fernando |
| author |
Arroyo Cordero, Francisco Teodoro |
| author_facet |
Arroyo Cordero, Francisco Teodoro Moreno Onorato, Francisco Javier García Herdugo, Gregorio Santos García de Paredes, Berta de los Barrau García, Carmen Porras, María Blanco Ruiz, César Romero Muñoz, Fernando |
| author_role |
author |
| author2 |
Moreno Onorato, Francisco Javier García Herdugo, Gregorio Santos García de Paredes, Berta de los Barrau García, Carmen Porras, María Blanco Ruiz, César Romero Muñoz, Fernando |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Biología Celular |
| dc.subject.none.fl_str_mv |
Colletotrichum acutatum Fragaria ×ananassa Infection vesicle Invasion strategy Penetration peg |
| topic |
Colletotrichum acutatum Fragaria ×ananassa Infection vesicle Invasion strategy Penetration peg |
| description |
The early stages of the infection of attached leaves and petioles of strawberry (Fragaria ×ananassa Duch. ‘Camarosa’) by Colletotrichum acutatum Simmonds were studied using scanning and transmission electron microscopy. Pre-penetration events of these tissues were similar, but the production of secondary conidia (microcyclic conidiation) was detected only on leaves. At the ultrastructural level, different stages of maturation of appressoria were observed and described. In young appressoria, the cell wall was composed of two layers and the plasma membrane displayed a wavy appearance. In the following stage, the appressorium developed a third electron-transparent layer between the cell wall and the plasma membrane. This new electron-transparent material was especially visible in the region of the appressorium near the cuticle. The plasma membrane of this appressorium showed a smooth appearance. Afterwards, a penetration peg emerged through the pore penetrating the cuticle and reached the epidermal wall where it enlarged to form an intramural infection vesicle. Both structures of infection, the penetration peg and the intramural infection vesicle, produced during the early phases of infection of strawbery tissues by C. acutatum, have not been previously reported and confirm that its invasion strategy is that of a subcuticular intramural pathogen. Once the infection was well established, abundant subcuticular and intramural hyphae were produced on petioles, causing severe degradation of the host cell walls. Occasionally, the cuticle appeared disrupted in those regions where the host walls were very degraded and dilated. Differences between colonization of petioles and leaves were observed. |
| publishDate |
2005 |
| dc.date.none.fl_str_mv |
2005 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11441/40306 https://doi.org/10.1139/B05-022 |
| url |
http://hdl.handle.net/11441/40306 https://doi.org/10.1139/B05-022 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
National Research Council of Canada |
| publisher.none.fl_str_mv |
National Research Council of Canada |
| dc.source.none.fl_str_mv |
reponame:idUS. Depósito de Investigación de la Universidad de Sevilla instname:Universidad de Sevilla (US) |
| instname_str |
Universidad de Sevilla (US) |
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idUS. Depósito de Investigación de la Universidad de Sevilla |
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idUS. Depósito de Investigación de la Universidad de Sevilla |
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|
| repository.mail.fl_str_mv |
|
| _version_ |
1869405781392097280 |
| spelling |
Ultrastructure of the early stages of Colletotrichum acutatum infection of strawberry tissuesArroyo Cordero, Francisco TeodoroMoreno Onorato, Francisco JavierGarcía Herdugo, GregorioSantos García de Paredes, Berta de losBarrau García, CarmenPorras, MaríaBlanco Ruiz, CésarRomero Muñoz, FernandoColletotrichum acutatumFragaria ×ananassaInfection vesicleInvasion strategyPenetration pegThe early stages of the infection of attached leaves and petioles of strawberry (Fragaria ×ananassa Duch. ‘Camarosa’) by Colletotrichum acutatum Simmonds were studied using scanning and transmission electron microscopy. Pre-penetration events of these tissues were similar, but the production of secondary conidia (microcyclic conidiation) was detected only on leaves. At the ultrastructural level, different stages of maturation of appressoria were observed and described. In young appressoria, the cell wall was composed of two layers and the plasma membrane displayed a wavy appearance. In the following stage, the appressorium developed a third electron-transparent layer between the cell wall and the plasma membrane. This new electron-transparent material was especially visible in the region of the appressorium near the cuticle. The plasma membrane of this appressorium showed a smooth appearance. Afterwards, a penetration peg emerged through the pore penetrating the cuticle and reached the epidermal wall where it enlarged to form an intramural infection vesicle. Both structures of infection, the penetration peg and the intramural infection vesicle, produced during the early phases of infection of strawbery tissues by C. acutatum, have not been previously reported and confirm that its invasion strategy is that of a subcuticular intramural pathogen. Once the infection was well established, abundant subcuticular and intramural hyphae were produced on petioles, causing severe degradation of the host cell walls. Occasionally, the cuticle appeared disrupted in those regions where the host walls were very degraded and dilated. Differences between colonization of petioles and leaves were observed.À l’aide de la microscopie électronique par balayage et par transmission, les auteurs ont étudié les premiers stades de l’infection des feuilles attachées et des pétioles du fraisier (Fragaria ×ananassa Duch. ‘Camarosa’), par le Colletotrichum acutatum Simmonds. Les évènements de pré-pénétration de ces tissus sont similaires, mais la production de conidies secondaires (conidiation microcyclique) ne s’observe que sur les feuilles. Les auteurs ont observé et décrivent les ultrastructures, aux différents stades de maturation des appressoriums. Chez les jeunes appressoriums, la paroi cellulaire est composée de deux couches et la membrane plasmique montre une apparence ondulée. Au stade suivant, l’appressorium développe une troisième couche transparente aux électrons, entre la paroi cellulaire et la plasmalemme. Ce nouveau matériel transparent aux électrons est particulièrement visible dans la région de l’appressorium voisin de la cuticule. La plasmalemme de cet appressorium est d’apparence lisse. Par après, l’hyphe de pénétration émerge à travers le pore et pénètre la cuticule pour atteindre la paroi épidermique, où il s’élargit pour former une vésicule d’infection intramurale. Les deux structures d’infection, hyphe de pénétration et vésicule d’infection intramurale, produites au cours des premières phases d’infection des tissus du fraisier par le C. acutatum, n’ont jamais été rapportées jusqu’ici et confirment sa stratégie d’invasion par pathogénèse subcuticulaire intra murale. Une fois l’infection bien établie, on observe la formation de nombreux hyphes subcuticulaires et intramuraux sur les pétioles, entraînant la dégradation des parois des cellules de l’hôte. Occasionnellement, la cuticule apparaît éclatée dans les régions où les parois de l’hôte sont vraiment dégradées et dilatées. On observe des différences entre la colonisation des pétioles et des feuilles.National Research Council of CanadaBiología Celular2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/40306https://doi.org/10.1139/B05-022reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)Inglésinfo:eu-repo/semantics/openAccessoai:idus.us.es:11441/403062026-06-17T12:51:07Z |
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15,300719 |