Comparative sensitivity of tumor and non-tumor cell lines as a reliable approach for in vitro cytotoxicity screening of lysine-based surfactants with potential pharmaceutical applications

Surfactants are used as additives in topical pharmaceuticals and drug delivery systems. The biocompatibility of amino acid-based surfactants makes them highly suitable for use in these fields, but tests are needed to evaluate their potential toxicity. Here we addressed the sensitivity of tumor (HeLa...

Descripción completa

Detalles Bibliográficos
Autores: Nogueira, Daniele Rubert, Mitjans, Montserrat, Infante, María Rosa, Vinardell, M. Pilar
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/332651
Acceso en línea:http://hdl.handle.net/10261/332651
https://api.elsevier.com/content/abstract/scopus_id/80054705804
Access Level:acceso abierto
Palabra clave:Cell sensitivity
Counterions
Cytotoxicity
Lysine-based surfactants
MTT
NRU
id ES_33f5692fc172a976dc28c03e79013a4e
oai_identifier_str oai:digital.csic.es:10261/332651
network_acronym_str ES
network_name_str España
repository_id_str
spelling Comparative sensitivity of tumor and non-tumor cell lines as a reliable approach for in vitro cytotoxicity screening of lysine-based surfactants with potential pharmaceutical applicationsNogueira, Daniele RubertMitjans, MontserratInfante, María RosaVinardell, M. PilarCell sensitivityCounterionsCytotoxicityLysine-based surfactantsMTTNRUSurfactants are used as additives in topical pharmaceuticals and drug delivery systems. The biocompatibility of amino acid-based surfactants makes them highly suitable for use in these fields, but tests are needed to evaluate their potential toxicity. Here we addressed the sensitivity of tumor (HeLa, MCF-7) and non-tumor (3T3, 3T6, HaCaT, NCTC 2544) cell lines to the toxic effects of lysine-based surfactants by means of two in vitro endpoints (MTT and NRU). This comparative assay may serve as a reliable approach for predictive toxicity screening of chemicals prior to pharmaceutical applications. After 24-h of cell exposure to surfactants, differing toxic responses were observed. NCTC 2544 and 3T6 cell lines were the most sensitive, while both tumor cells and 3T3 fibroblasts were more resistant to the cytotoxic effects of surfactants. IC(50)-values revealed that cytotoxicity was detected earlier by MTT assay than by NRU assay, regardless of the compound or cell line. The overall results showed that surfactants with organic counterions were less cytotoxic than those with inorganic counterions. Our findings highlight the relevance of the correct choice and combination of cell lines and bioassays in toxicity studies for a safe and reliable screen of chemicals with potential interest in pharmaceutical industry.This research was supported by Project CTQ2009-14151-C02-02 from Ministerio de Ciencia e Innovación (Spain). Daniele Rubert Nogueira holds doctoral grant from MAEC-AECID (Spain).Peer reviewedElsevier BVComisión Asesora de Investigación Científica y Técnica, CAICYT (España)Ministerio de Asuntos Exteriores y Cooperación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/332651https://api.elsevier.com/content/abstract/scopus_id/80054705804reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.ijpharm.2011.08.020Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3326512026-05-22T06:33:51Z
dc.title.none.fl_str_mv Comparative sensitivity of tumor and non-tumor cell lines as a reliable approach for in vitro cytotoxicity screening of lysine-based surfactants with potential pharmaceutical applications
title Comparative sensitivity of tumor and non-tumor cell lines as a reliable approach for in vitro cytotoxicity screening of lysine-based surfactants with potential pharmaceutical applications
spellingShingle Comparative sensitivity of tumor and non-tumor cell lines as a reliable approach for in vitro cytotoxicity screening of lysine-based surfactants with potential pharmaceutical applications
Nogueira, Daniele Rubert
Cell sensitivity
Counterions
Cytotoxicity
Lysine-based surfactants
MTT
NRU
title_short Comparative sensitivity of tumor and non-tumor cell lines as a reliable approach for in vitro cytotoxicity screening of lysine-based surfactants with potential pharmaceutical applications
title_full Comparative sensitivity of tumor and non-tumor cell lines as a reliable approach for in vitro cytotoxicity screening of lysine-based surfactants with potential pharmaceutical applications
title_fullStr Comparative sensitivity of tumor and non-tumor cell lines as a reliable approach for in vitro cytotoxicity screening of lysine-based surfactants with potential pharmaceutical applications
title_full_unstemmed Comparative sensitivity of tumor and non-tumor cell lines as a reliable approach for in vitro cytotoxicity screening of lysine-based surfactants with potential pharmaceutical applications
title_sort Comparative sensitivity of tumor and non-tumor cell lines as a reliable approach for in vitro cytotoxicity screening of lysine-based surfactants with potential pharmaceutical applications
dc.creator.none.fl_str_mv Nogueira, Daniele Rubert
Mitjans, Montserrat
Infante, María Rosa
Vinardell, M. Pilar
author Nogueira, Daniele Rubert
author_facet Nogueira, Daniele Rubert
Mitjans, Montserrat
Infante, María Rosa
Vinardell, M. Pilar
author_role author
author2 Mitjans, Montserrat
Infante, María Rosa
Vinardell, M. Pilar
author2_role author
author
author
dc.contributor.none.fl_str_mv Comisión Asesora de Investigación Científica y Técnica, CAICYT (España)
Ministerio de Asuntos Exteriores y Cooperación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cell sensitivity
Counterions
Cytotoxicity
Lysine-based surfactants
MTT
NRU
topic Cell sensitivity
Counterions
Cytotoxicity
Lysine-based surfactants
MTT
NRU
description Surfactants are used as additives in topical pharmaceuticals and drug delivery systems. The biocompatibility of amino acid-based surfactants makes them highly suitable for use in these fields, but tests are needed to evaluate their potential toxicity. Here we addressed the sensitivity of tumor (HeLa, MCF-7) and non-tumor (3T3, 3T6, HaCaT, NCTC 2544) cell lines to the toxic effects of lysine-based surfactants by means of two in vitro endpoints (MTT and NRU). This comparative assay may serve as a reliable approach for predictive toxicity screening of chemicals prior to pharmaceutical applications. After 24-h of cell exposure to surfactants, differing toxic responses were observed. NCTC 2544 and 3T6 cell lines were the most sensitive, while both tumor cells and 3T3 fibroblasts were more resistant to the cytotoxic effects of surfactants. IC(50)-values revealed that cytotoxicity was detected earlier by MTT assay than by NRU assay, regardless of the compound or cell line. The overall results showed that surfactants with organic counterions were less cytotoxic than those with inorganic counterions. Our findings highlight the relevance of the correct choice and combination of cell lines and bioassays in toxicity studies for a safe and reliable screen of chemicals with potential interest in pharmaceutical industry.
publishDate 2011
dc.date.none.fl_str_mv 2011
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/332651
https://api.elsevier.com/content/abstract/scopus_id/80054705804
url http://hdl.handle.net/10261/332651
https://api.elsevier.com/content/abstract/scopus_id/80054705804
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.ijpharm.2011.08.020
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405780205109248
score 15,812429

Similares en LA Referencia