Spatio-temporal dynamics of intercellular Notch signaling: a modeling approach
[eng] Intercellular signalling is a mechanism by which cells communicate and mediate coordinated responses. In this Thesis we perform theoretical and computational analyses to evaluate spatio-temporal organizations that can arise in developing embryos through juxtacrine signalling mediated by Notch...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/107214 |
| Acceso en línea: | https://hdl.handle.net/2445/107214 http://hdl.handle.net/10803/400610 |
| Access Level: | acceso abierto |
| Palabra clave: | Proteïnes de membrana Diferenciació cel·lular Membrane proteins Cell diferentiation |
| Sumario: | [eng] Intercellular signalling is a mechanism by which cells communicate and mediate coordinated responses. In this Thesis we perform theoretical and computational analyses to evaluate spatio-temporal organizations that can arise in developing embryos through juxtacrine signalling mediated by Notch receptor. Juxtacrine signalling is a type of intercellular communication in which adjacent cells mutually interact through membrane-attached proteins (receptors and ligands). In the case of Notch signalling, the ligands are regulated by the signalling, establishing a feedback loop on the activation of the signal between adjacent cells. Our study focused on the following open questions: 1. What coordinated responses arise when Notch signalling is activated by two (or more) co-expressing ligands? 2. What is the effect on the response mediated by Notch signalling when considering a gene involved in cell differentiation that promotes it on transcription, but in turn is repressed by the signal? 3. Which processes can modulate the signalling efficiency of a ligand? These questions encompass aspects occurring at different levels, ranging from the type of response that arises in a tissue (salt-and-pepper patterning, homogeneous states or propagation of a cell fate in an expansive wave), the signalling state of cells (sending, receiver, among others), up to the dynamics of the intracellular processing of Notch. We used a modelling and computational approach for their study and compared, when possible, with previously reported phenotypes in developing embryos. |
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