Analysis of the xanthans, produced by Xanthomonas albilineans, by capillary electrophoresis: Identification of the product of reaction of an UDP-glucose dehydrogenase
Leaf scald, a bacterial-vascular disease of sugar cane, has Xanthomonas albilineans as casual organism. The organism may invade the parenchyma between the bundles and produces reddened pockets of gum, identified as a xanthan. This xanthan consists of a basal tetramer composed by two molecules of glu...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2009 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/91765 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/91765 |
| Access Level: | acceso abierto |
| Palabra clave: | Capillary Electrophoresis UDP-glucuronic acid UDP-glucose dehydrogenase Sugarcane Xanthomonas albilineans Xanthans Fisiología vegetal (Biología) 3108 Fitopatología |
| Sumario: | Leaf scald, a bacterial-vascular disease of sugar cane, has Xanthomonas albilineans as casual organism. The organism may invade the parenchyma between the bundles and produces reddened pockets of gum, identified as a xanthan. This xanthan consists of a basal tetramer composed by two molecules of glucose, one mannose rest and a final glucuronic acid that is highly repeated to form the macromolecule. The occurrence of glucuronate rest in the polysaccharide requires the action of bacterial UDP-glucose dehydrogenase. In order to verify that the obtained protein is a true UDP-glucose dehydrogenase, the identification of the UDP-glucuronic acid like only product of reaction was necessary. The detection of the UDP-glucuronic acid has been carried out by Capillary Electrophoresis. |
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