Selenium Agronomic Biofortification of Durum Wheat Fertilized with Organic Products: Se Content and Speciation in Grain

The biofortification of cereals is a potential solution for increasing Se levels in the human diet. Here we evaluated whether applying selenate and organic N and S sources via foliar alters Se content and species in durum wheat grain. Field trials were conducted in a Mediterranean environment in 201...

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Detalles Bibliográficos
Autores: Carucci, Federica, Moreno Martín, Gustavo, Madrid-Albarrán, Yolanda, Gatta, Giuseppe, De Vita, Pasquale, Michela Giuliani, Marcella
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/72210
Acceso en línea:https://hdl.handle.net/20.500.14352/72210
Access Level:acceso abierto
Palabra clave:543
sodium selenate
nitrogen
sulphur
Triticum durum
SeMetSeCys
SeMet
foliar fertilization
ICP-MS
HPLC-ICP-MS
HPLC-ESI-MS/MS
Química analítica (Química)
2301 Química Analítica
Descripción
Sumario:The biofortification of cereals is a potential solution for increasing Se levels in the human diet. Here we evaluated whether applying selenate and organic N and S sources via foliar alters Se content and species in durum wheat grain. Field trials were conducted in a Mediterranean environment in 2017–2018 and 2018–2019 on old (Cappelli and old Saragolla) and modern (Marco Aurelio and Nadif) durum wheat varieties. Four organic fertilization strategies were evaluated: the control (dry blood meal at sowing), the application of foliar S (Bio-sulphur at flag leaf) and N (liquid blood meal at the beginning of heading), and their combined use in interaction with a foliar application of selenate at the booting stage. The Se content in grain was determined using ICP-MS and its speciation throughout HPLC-ICP-MS and HPLC-ESI-MS/MS. In 2018, the lower rainfall in the ten days before the Se foliar application caused a higher Se accumulation in the grain (1.63 vs. 1.44 mg kg−1 in 2017–2018 and 2018–2019, respectively). A negative effect of organic foliar S on Se content in grain was observed (−24% with respect to the control). The modern Nadif showed the highest Se content (1.87 mg kg−1), followed by the old variety Cappelli (1.57 mg kg−1). Finally, the Se speciation analysis showed the presence of SeMet and SeMetSeCys. The organic fertilization affected only SeMet, with lower values under organic S fertilization and higher values under organic N fertilization