Effect of Carbon, Nitrogen Sources and Abiotic Stress on Phosphate Solubilization by Bacterial Strains Isolated from a Moroccan Rock Phosphate Deposit

Abstract Experiments were conducted to evaluate the solubilization of tri-calcium phosphate (TCP) by phosphatesolubilizing bacterial isolates PSB 4, 5 and 6 identified as Enterobacter sp., Bacterium DR172, and Enterobacter hormaechei, respectively, in addition to Acinetobacter sp. used as a positive...

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Detalles Bibliográficos
Autores: Mardad, Illham, Serrano, Aurelio, Soukri, A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/178456
Acceso en línea:http://hdl.handle.net/10261/178456
Access Level:acceso abierto
Palabra clave:Carbon source
Enterobacter
Acinetobacter
Nitrogen source
Stress
Phosphate solubilization
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spelling Effect of Carbon, Nitrogen Sources and Abiotic Stress on Phosphate Solubilization by Bacterial Strains Isolated from a Moroccan Rock Phosphate DepositMardad, IllhamSerrano, AurelioSoukri, A.Carbon sourceEnterobacterAcinetobacterNitrogen sourceStressPhosphate solubilizationAbstract Experiments were conducted to evaluate the solubilization of tri-calcium phosphate (TCP) by phosphatesolubilizing bacterial isolates PSB 4, 5 and 6 identified as Enterobacter sp., Bacterium DR172, and Enterobacter hormaechei, respectively, in addition to Acinetobacter sp. used as a positive control strain. The study was carried out in culture media using different carbon and nitrogen sources, and under different abiotic stress conditions such as high salt, pH, and temperature. The effect of EDTA on growth was also tested. Organic acids produced by the PSB isolates were also determined by reverse phase HPLC. The isolates behave differently with different types of N- and C-sources and seem to adapt to different conditions, with glucose significantly promoting TCP solubilization in all cases. The highest production of orthophosphate (866 mg.L-1, pH 3.2) was showed by Enterobacter hormaechei using glucose as C-source, (NH4)2SO4 as N-source, at 37°C and pH 7, and without any addition of EDTA. The solubilized phosphate also did attaint 110.71% in comparison with control when sorbitol was used as a C-source by Bacterium DR172. We also noticed a decrease in pH due to the organic acids secreted to the medium. Newly carboxylic acids were identified to be produced by these strains in addition to others previously identified, 2 ketogluconic acid was found when galactose was used as a C-source for all isolates except for Acinetobacter sp. which secreted lactic, glutaric and glucuronic acids. Analysis of the Solubilization Index (SI) of the bacterial strains at different temperatures revealed different effectiveness to degrade and assimilate TCP, the SI being maximal at 25°C for the three isolates (4.17, 3.83 and 4.44, for PSB4, 5 and 6), whereas for Acinetobacter sp. the highest SI (3.83) was achieved at 30°C.Peer ReviewedLongdom PublishingConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920142019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/178456reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1784562026-05-22T06:33:51Z
dc.title.none.fl_str_mv Effect of Carbon, Nitrogen Sources and Abiotic Stress on Phosphate Solubilization by Bacterial Strains Isolated from a Moroccan Rock Phosphate Deposit
title Effect of Carbon, Nitrogen Sources and Abiotic Stress on Phosphate Solubilization by Bacterial Strains Isolated from a Moroccan Rock Phosphate Deposit
spellingShingle Effect of Carbon, Nitrogen Sources and Abiotic Stress on Phosphate Solubilization by Bacterial Strains Isolated from a Moroccan Rock Phosphate Deposit
Mardad, Illham
Carbon source
Enterobacter
Acinetobacter
Nitrogen source
Stress
Phosphate solubilization
title_short Effect of Carbon, Nitrogen Sources and Abiotic Stress on Phosphate Solubilization by Bacterial Strains Isolated from a Moroccan Rock Phosphate Deposit
title_full Effect of Carbon, Nitrogen Sources and Abiotic Stress on Phosphate Solubilization by Bacterial Strains Isolated from a Moroccan Rock Phosphate Deposit
title_fullStr Effect of Carbon, Nitrogen Sources and Abiotic Stress on Phosphate Solubilization by Bacterial Strains Isolated from a Moroccan Rock Phosphate Deposit
title_full_unstemmed Effect of Carbon, Nitrogen Sources and Abiotic Stress on Phosphate Solubilization by Bacterial Strains Isolated from a Moroccan Rock Phosphate Deposit
title_sort Effect of Carbon, Nitrogen Sources and Abiotic Stress on Phosphate Solubilization by Bacterial Strains Isolated from a Moroccan Rock Phosphate Deposit
dc.creator.none.fl_str_mv Mardad, Illham
Serrano, Aurelio
Soukri, A.
author Mardad, Illham
author_facet Mardad, Illham
Serrano, Aurelio
Soukri, A.
author_role author
author2 Serrano, Aurelio
Soukri, A.
author2_role author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Carbon source
Enterobacter
Acinetobacter
Nitrogen source
Stress
Phosphate solubilization
topic Carbon source
Enterobacter
Acinetobacter
Nitrogen source
Stress
Phosphate solubilization
description Abstract Experiments were conducted to evaluate the solubilization of tri-calcium phosphate (TCP) by phosphatesolubilizing bacterial isolates PSB 4, 5 and 6 identified as Enterobacter sp., Bacterium DR172, and Enterobacter hormaechei, respectively, in addition to Acinetobacter sp. used as a positive control strain. The study was carried out in culture media using different carbon and nitrogen sources, and under different abiotic stress conditions such as high salt, pH, and temperature. The effect of EDTA on growth was also tested. Organic acids produced by the PSB isolates were also determined by reverse phase HPLC. The isolates behave differently with different types of N- and C-sources and seem to adapt to different conditions, with glucose significantly promoting TCP solubilization in all cases. The highest production of orthophosphate (866 mg.L-1, pH 3.2) was showed by Enterobacter hormaechei using glucose as C-source, (NH4)2SO4 as N-source, at 37°C and pH 7, and without any addition of EDTA. The solubilized phosphate also did attaint 110.71% in comparison with control when sorbitol was used as a C-source by Bacterium DR172. We also noticed a decrease in pH due to the organic acids secreted to the medium. Newly carboxylic acids were identified to be produced by these strains in addition to others previously identified, 2 ketogluconic acid was found when galactose was used as a C-source for all isolates except for Acinetobacter sp. which secreted lactic, glutaric and glucuronic acids. Analysis of the Solubilization Index (SI) of the bacterial strains at different temperatures revealed different effectiveness to degrade and assimilate TCP, the SI being maximal at 25°C for the three isolates (4.17, 3.83 and 4.44, for PSB4, 5 and 6), whereas for Acinetobacter sp. the highest SI (3.83) was achieved at 30°C.
publishDate 2014
dc.date.none.fl_str_mv 2014
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/178456
url http://hdl.handle.net/10261/178456
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Longdom Publishing
publisher.none.fl_str_mv Longdom Publishing
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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