Immunogenicity and protection against Mycobacterium caprae challenge in goats vaccinated with BCG and revaccinated after one year

Vaccination has been proposed as a supplementary tool for the control of tuberculosis in livestock. The long-term immunogenicity elicited by bacillus Calmette-Guerin (BCG) and the efficacy of revaccination were investigated in thirty goat kids distributed into three groups: unvaccinated controls, BC...

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Authors: Arrieta-Villegas, Claudia, Vidal, Enric, Martín, Maite, Verdés, Judit, Moll, Xavier, Espada, Yvonne, Singh, Mahavir, Villarreal-Ramos, Bernardo, Domingo, Mariano, Pérez de Val, Bernat
Format: article
Publication Date:2020
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/1063
Online Access:http://hdl.handle.net/20.500.12327/1063
https://doi.org/10.3390/vaccines8040751
Access Level:Open access
Keyword:619
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spelling Immunogenicity and protection against Mycobacterium caprae challenge in goats vaccinated with BCG and revaccinated after one yearArrieta-Villegas, ClaudiaVidal, EnricMartín, MaiteVerdés, JuditMoll, XavierEspada, YvonneSingh, MahavirVillarreal-Ramos, BernardoDomingo, MarianoPérez de Val, Bernat619Vaccination has been proposed as a supplementary tool for the control of tuberculosis in livestock. The long-term immunogenicity elicited by bacillus Calmette-Guerin (BCG) and the efficacy of revaccination were investigated in thirty goat kids distributed into three groups: unvaccinated controls, BCG (vaccinated at week 0) and BCG-BCG (vaccinated at weeks 0 and 56). Sixty-four weeks after the first vaccination, all animals were challenged with Mycobacterium caprae and examined post-mortem (pathology and bacterial load) at week 73. Antigen-specific interferon-gamma (IFN-γ) release was measured throughout the experiment. At week 59, peripheral blood mononuclear cells were stained for CD4, CD45RO and IFN-γ to determine the presence of antigen-specific cells secreting IFN-γ. The BCG-BCG group showed reductions in rectal temperatures, M. caprae DNA load in pulmonary lymph nodes (LN), the volume of lesions in pulmonary LN, mineralization in lungs, and higher weight gains compared to unvaccinated controls. IFN-γ responses were undetectable from 32 weeks after primary vaccination until revaccination, when the BCG-BCG group showed detectable IFN-γ production and a greater percentage of antigen-specific CD4+CD45RO+IFNγ+ and CD4-CD45RO+IFNγ+ cells compared to the BCG and control groups, which may be an indicator of the mechanisms of protection. Thus, re-vaccination of goats with BCG appears to prolong protection against infection with M. caprae.info:eu-repo/semantics/publishedVersionMDPIProducció AnimalSanitat Animal2020info:eu-repo/semantics/article16http://hdl.handle.net/20.500.12327/1063https://doi.org/10.3390/vaccines8040751reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVaccinesINIA-FEDER/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2015-0043-C02-01/ES/Estudio de vacunas, estrategias de vacunación y nuevas herramientas de lucha frente a la tuberculosis en la especie caprina: Estrategia DIVA/Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/10632026-05-29T05:05:01Z
dc.title.none.fl_str_mv Immunogenicity and protection against Mycobacterium caprae challenge in goats vaccinated with BCG and revaccinated after one year
title Immunogenicity and protection against Mycobacterium caprae challenge in goats vaccinated with BCG and revaccinated after one year
spellingShingle Immunogenicity and protection against Mycobacterium caprae challenge in goats vaccinated with BCG and revaccinated after one year
Arrieta-Villegas, Claudia
619
title_short Immunogenicity and protection against Mycobacterium caprae challenge in goats vaccinated with BCG and revaccinated after one year
title_full Immunogenicity and protection against Mycobacterium caprae challenge in goats vaccinated with BCG and revaccinated after one year
title_fullStr Immunogenicity and protection against Mycobacterium caprae challenge in goats vaccinated with BCG and revaccinated after one year
title_full_unstemmed Immunogenicity and protection against Mycobacterium caprae challenge in goats vaccinated with BCG and revaccinated after one year
title_sort Immunogenicity and protection against Mycobacterium caprae challenge in goats vaccinated with BCG and revaccinated after one year
dc.creator.none.fl_str_mv Arrieta-Villegas, Claudia
Vidal, Enric
Martín, Maite
Verdés, Judit
Moll, Xavier
Espada, Yvonne
Singh, Mahavir
Villarreal-Ramos, Bernardo
Domingo, Mariano
Pérez de Val, Bernat
author Arrieta-Villegas, Claudia
author_facet Arrieta-Villegas, Claudia
Vidal, Enric
Martín, Maite
Verdés, Judit
Moll, Xavier
Espada, Yvonne
Singh, Mahavir
Villarreal-Ramos, Bernardo
Domingo, Mariano
Pérez de Val, Bernat
author_role author
author2 Vidal, Enric
Martín, Maite
Verdés, Judit
Moll, Xavier
Espada, Yvonne
Singh, Mahavir
Villarreal-Ramos, Bernardo
Domingo, Mariano
Pérez de Val, Bernat
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Sanitat Animal
dc.subject.none.fl_str_mv 619
topic 619
description Vaccination has been proposed as a supplementary tool for the control of tuberculosis in livestock. The long-term immunogenicity elicited by bacillus Calmette-Guerin (BCG) and the efficacy of revaccination were investigated in thirty goat kids distributed into three groups: unvaccinated controls, BCG (vaccinated at week 0) and BCG-BCG (vaccinated at weeks 0 and 56). Sixty-four weeks after the first vaccination, all animals were challenged with Mycobacterium caprae and examined post-mortem (pathology and bacterial load) at week 73. Antigen-specific interferon-gamma (IFN-γ) release was measured throughout the experiment. At week 59, peripheral blood mononuclear cells were stained for CD4, CD45RO and IFN-γ to determine the presence of antigen-specific cells secreting IFN-γ. The BCG-BCG group showed reductions in rectal temperatures, M. caprae DNA load in pulmonary lymph nodes (LN), the volume of lesions in pulmonary LN, mineralization in lungs, and higher weight gains compared to unvaccinated controls. IFN-γ responses were undetectable from 32 weeks after primary vaccination until revaccination, when the BCG-BCG group showed detectable IFN-γ production and a greater percentage of antigen-specific CD4+CD45RO+IFNγ+ and CD4-CD45RO+IFNγ+ cells compared to the BCG and control groups, which may be an indicator of the mechanisms of protection. Thus, re-vaccination of goats with BCG appears to prolong protection against infection with M. caprae.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/1063
https://doi.org/10.3390/vaccines8040751
url http://hdl.handle.net/20.500.12327/1063
https://doi.org/10.3390/vaccines8040751
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Vaccines
INIA-FEDER/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2015-0043-C02-01/ES/Estudio de vacunas, estrategias de vacunación y nuevas herramientas de lucha frente a la tuberculosis en la especie caprina: Estrategia DIVA/
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 16
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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