Mechanics of epithelial closure over non-adherent environments

The closure of gaps within epithelia is crucial to maintain its integrity during biological processes such as wound healing and gastrulation. Depending on the distribution of extracellular matrix, gap closure occurs through assembly of multicellular actin-based contractile cables or protrusive activ...

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Autores: Vedula, Sri Ram Krishna, Peyret, Grégoire, Cheddadi, Ibrahim, Chen, Tianchi, Brugués, Agustí, Hirata, Hiroaki, López Menéndez Horacio, Toyama, Yusuke, Almeida, Luis Neves de, Trepat Guixer, Xavier, Lim, Chwee Teck, Ladoux, Benoit
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/118609
Acceso en línea:https://hdl.handle.net/2445/118609
Access Level:acceso abierto
Palabra clave:Cèl·lules epitelials
Biomecànica
Teixits (Histologia)
Epithelial cells
Biomechanics
Tissues
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spelling Mechanics of epithelial closure over non-adherent environmentsVedula, Sri Ram KrishnaPeyret, GrégoireCheddadi, IbrahimChen, TianchiBrugués, AgustíHirata, HiroakiLópez Menéndez HoracioToyama, YusukeAlmeida, Luis Neves deTrepat Guixer, XavierLim, Chwee TeckLadoux, BenoitCèl·lules epitelialsBiomecànicaTeixits (Histologia)Epithelial cellsBiomechanicsTissuesThe closure of gaps within epithelia is crucial to maintain its integrity during biological processes such as wound healing and gastrulation. Depending on the distribution of extracellular matrix, gap closure occurs through assembly of multicellular actin-based contractile cables or protrusive activity of border cells into the gap. Here we show that the supracellular actomyosin contractility of cells near the gap edge exerts sufficient tension on the surrounding tissue to promote closure of non-adherent gaps. Using traction force microscopy, we observe that cell-generated forces on the substrate at the gap edge first point away from the centre of the gap and then increase in the radial direction pointing into the gap as closure proceeds. Combining with numerical simulations, we show that the increase in force relies less on localized purse-string contractility and more on large-scale remodelling of the suspended tissue around the gap. Our results provide a framework for understanding the assembly and the mechanics of cellular contractility at the tissue level.Nature Publishing Group2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/118609Articles publicats en revistes (Biomedicina)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1038/ncomms7111Nature Communications, 2015, vol. 6, p. 6111https://doi.org/10.1038/ncomms7111info:eu-repo/grantAgreement/EC/FP7/242993info:eu-repo/grantAgreement/EC/FP7/617233cc-by (c) Vedula, Sri Ram Krishna et al., 2015http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1186092026-05-27T06:46:51Z
dc.title.none.fl_str_mv Mechanics of epithelial closure over non-adherent environments
title Mechanics of epithelial closure over non-adherent environments
spellingShingle Mechanics of epithelial closure over non-adherent environments
Vedula, Sri Ram Krishna
Cèl·lules epitelials
Biomecànica
Teixits (Histologia)
Epithelial cells
Biomechanics
Tissues
title_short Mechanics of epithelial closure over non-adherent environments
title_full Mechanics of epithelial closure over non-adherent environments
title_fullStr Mechanics of epithelial closure over non-adherent environments
title_full_unstemmed Mechanics of epithelial closure over non-adherent environments
title_sort Mechanics of epithelial closure over non-adherent environments
dc.creator.none.fl_str_mv Vedula, Sri Ram Krishna
Peyret, Grégoire
Cheddadi, Ibrahim
Chen, Tianchi
Brugués, Agustí
Hirata, Hiroaki
López Menéndez Horacio
Toyama, Yusuke
Almeida, Luis Neves de
Trepat Guixer, Xavier
Lim, Chwee Teck
Ladoux, Benoit
author Vedula, Sri Ram Krishna
author_facet Vedula, Sri Ram Krishna
Peyret, Grégoire
Cheddadi, Ibrahim
Chen, Tianchi
Brugués, Agustí
Hirata, Hiroaki
López Menéndez Horacio
Toyama, Yusuke
Almeida, Luis Neves de
Trepat Guixer, Xavier
Lim, Chwee Teck
Ladoux, Benoit
author_role author
author2 Peyret, Grégoire
Cheddadi, Ibrahim
Chen, Tianchi
Brugués, Agustí
Hirata, Hiroaki
López Menéndez Horacio
Toyama, Yusuke
Almeida, Luis Neves de
Trepat Guixer, Xavier
Lim, Chwee Teck
Ladoux, Benoit
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cèl·lules epitelials
Biomecànica
Teixits (Histologia)
Epithelial cells
Biomechanics
Tissues
topic Cèl·lules epitelials
Biomecànica
Teixits (Histologia)
Epithelial cells
Biomechanics
Tissues
description The closure of gaps within epithelia is crucial to maintain its integrity during biological processes such as wound healing and gastrulation. Depending on the distribution of extracellular matrix, gap closure occurs through assembly of multicellular actin-based contractile cables or protrusive activity of border cells into the gap. Here we show that the supracellular actomyosin contractility of cells near the gap edge exerts sufficient tension on the surrounding tissue to promote closure of non-adherent gaps. Using traction force microscopy, we observe that cell-generated forces on the substrate at the gap edge first point away from the centre of the gap and then increase in the radial direction pointing into the gap as closure proceeds. Combining with numerical simulations, we show that the increase in force relies less on localized purse-string contractility and more on large-scale remodelling of the suspended tissue around the gap. Our results provide a framework for understanding the assembly and the mechanics of cellular contractility at the tissue level.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/118609
url https://hdl.handle.net/2445/118609
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/ncomms7111
Nature Communications, 2015, vol. 6, p. 6111
https://doi.org/10.1038/ncomms7111
info:eu-repo/grantAgreement/EC/FP7/242993
info:eu-repo/grantAgreement/EC/FP7/617233
dc.rights.none.fl_str_mv cc-by (c) Vedula, Sri Ram Krishna et al., 2015
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Vedula, Sri Ram Krishna et al., 2015
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301629

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