Exacerbated response to oxidative stress in the Retinitis Pigmentosa CerklKD/KO mouse model triggers retinal degeneration pathways upon acute light stress
The retina is particularly vulnerable to genetic and environmental alterations that generate oxidative stress and cause cellular damage in photoreceptors and other retinal neurons, eventually leading to cell death. <em>CERKL </em>(<em>CERamide Kinase-Like</em>) mutations caus...
| Autores: | , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/206412 |
| Acceso en línea: | https://hdl.handle.net/2445/206412 |
| Access Level: | acceso abierto |
| Palabra clave: | Malalties de la retina Estrès oxidatiu Retinal diseases Oxidative stress |
| Sumario: | The retina is particularly vulnerable to genetic and environmental alterations that generate oxidative stress and cause cellular damage in photoreceptors and other retinal neurons, eventually leading to cell death. <em>CERKL </em>(<em>CERamide Kinase-Like</em>) mutations cause Retinitis Pigmentosa and Cone-Rod Dystrophy in humans, two disorders characterized by photoreceptor degeneration and progressive vision loss. <em>CERKL </em>is a resilience gene against oxidative stress, and its overexpression protects cells from oxidative stress-induced apoptosis. Besides, CERKL contributes to stress granule-formation and regulates mitochondrial dynamics in the retina. Using the <em>CerklKD/KO </em>albino mouse model, which recapitulates the human disease, we aimed to study the impact of <em>Cerkl </em>knockdown on stress response and activation of photoreceptor death mechanisms upon light/oxidative stress. After acute light injury, we assessed immediate or late retinal stress response, by combining both omic and non-omic approaches. Our results show that <em>Cerkl </em>knockdown increases ROS levels and causes a basal exacerbated stress state in the retina, through alterations in glutathione metabolism and stress granule production, overall compromising an adequate response to additional oxidative damage. As a consequence, several cell death mechanisms are triggered in <em>CerklKD/KO </em>retinas after acute light stress. Our studies indicate that <em>Cerkl </em>gene is a pivotal player in regulating light-challenged retinal homeostasis and shed light on how mutations in <em>CERKL </em>lead to blindness by dysregulation of the basal oxidative stress response in the retina. |
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