Clinical course impacts early kinetics,magnitude, and amplitude of SARS-CoV-2 neutralizing antibodies beyond 1 year after infection

To understand the determinants of long-term immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the concurrent impact of vaccination and emerging variants, we follow a prospective cohort of 332 patients with coronavirus disease 2019 (COVID-19) over more than a year a...

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Bibliographic Details
Authors: Pradenas, Edwards, Trinité, Benjamin, Urrea, Víctor, Marfil, Silvia, Tarrés Freixas, Ferran, Guallar, Víctor|||0000-0002-4580-1114, Valencia, Alfonso|||0000-0002-8937-6789
Format: article
Publication Date:2022
Country:España
Institution:Universitat Politècnica de Catalunya (UPC)
Repository:UPCommons. Portal del coneixement obert de la UPC
Language:English
OAI Identifier:oai:upcommons.upc.edu:2117/363107
Online Access:https://hdl.handle.net/2117/363107
https://dx.doi.org/10.1016/j.xcrm.2022.100523
Access Level:Open access
Keyword:SARS-CoV-2 disease
Acute respiratory syndrome, Severe
Vaccination
SARS-CoV-2
Humoral response
B cell memory
Pseudovirus
Neutralizing antibodies
Variants of concern
Severity
Durability
Half-life
SARS-CoV-2 (Malaltia)
Àrees temàtiques de la UPC::Informàtica::Aplicacions de la informàtica::Bioinformàtica
Description
Summary:To understand the determinants of long-term immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the concurrent impact of vaccination and emerging variants, we follow a prospective cohort of 332 patients with coronavirus disease 2019 (COVID-19) over more than a year after symptom onset. We evaluate plasma-neutralizing activity using HIV-based pseudoviruses expressing the spike of different SARS-CoV-2 variants and analyze them longitudinally using mixed-effects models. Long-term neutralizing activity is stable beyond 1 year after infection in mild/asymptomatic and hospitalized participants. However, longitudinal models suggest that hospitalized individuals generate both short- and long-lived memory B cells, while the responses of non-hospitalized individuals are dominated by long-lived B cells. In both groups, vaccination boosts responses to natural infection. Long-term (>300 days from infection) responses in unvaccinated participants show a reduced efficacy against beta, but not alpha nor delta, variants. Multivariate analysis identifies the severity of primary infection as an independent determinant of higher magnitude and lower relative cross-neutralization activity of long-term neutralizing responses.