Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development

Free and glycosylated sterols localize in the plant cell plasma membrane, where in combination with other lipids regulate its structure and function. The role of glycosylated sterols in regulating membrane-associated biological processes is more relevant in plants like tomato (Solanum lycopersicum),...

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Autores: Chávez, Ángel, Castillo, Nídia, López-Tubau, Joan Manel, Atanasov, Kostadin Evgeniev, Fernández-Crespo, Emma, Camañes, Gemma, Altabella Artigas, Teresa, Ferrer i Prats, Albert
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/193496
Acceso en línea:https://hdl.handle.net/2445/193496
Access Level:acceso abierto
Palabra clave:Tomàquets
Arabidopsis
Glucòsids
Tomatoes
Glucosides
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spelling Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit developmentChávez, ÁngelCastillo, NídiaLópez-Tubau, Joan ManelAtanasov, Kostadin EvgenievFernández-Crespo, EmmaCamañes, GemmaAltabella Artigas, TeresaFerrer i Prats, AlbertTomàquetsArabidopsisGlucòsidsTomatoesArabidopsisGlucosidesFree and glycosylated sterols localize in the plant cell plasma membrane, where in combination with other lipids regulate its structure and function. The role of glycosylated sterols in regulating membrane-associated biological processes is more relevant in plants like tomato (Solanum lycopersicum), in which glycosylated sterols are the predominant sterols. A proper ratio of free sterols versus glycosylated sterols has proven to be essential for proper plant performance in several species, but almost nothing is known in tomato. To assess the role of glycosylated sterols in tomato plant and fruit development, we generated transgenic lines of tomato cultivar Micro-Tom expressing two different amiRNAs devised to silence STEROL GLYCOSYLTRANSFERASE 1, the most actively expressed of the four genes encoding sterol glycosyltransferases in this plant. STEROL GLYCOSYLTRANSFERASE 1 gene silencing caused moderate plant dwarfism and reduced fruit size. Analysis of the profile of glycosylated sterols throughout fruit development demonstrated that the maintenance of proper levels of these compounds during the early stages of fruit development is essential for normal fruit growth, since reduced levels of glycosylated sterols trigger a transcriptional downregulatory response that affects genes involved in processes that are critical for proper fruit development, such as seed filling, cell wall extension and auxin signaling.Elsevier B.V.2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/193496Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1016/j.envexpbot.2022.105181Environmental and Experimental Botany, 2022, vol. 206, num. 105181https://doi.org/10.1016/j.envexpbot.2022.105181cc-by-nc-nd (c) Ángel Chávez, et al, 2022https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1934962026-05-27T06:46:51Z
dc.title.none.fl_str_mv Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development
title Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development
spellingShingle Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development
Chávez, Ángel
Tomàquets
Arabidopsis
Glucòsids
Tomatoes
Arabidopsis
Glucosides
title_short Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development
title_full Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development
title_fullStr Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development
title_full_unstemmed Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development
title_sort Tomato STEROL GLYCOSYLTRANSFERASE 1 silencing unveils a major role of steryl glycosides in plant and fruit development
dc.creator.none.fl_str_mv Chávez, Ángel
Castillo, Nídia
López-Tubau, Joan Manel
Atanasov, Kostadin Evgeniev
Fernández-Crespo, Emma
Camañes, Gemma
Altabella Artigas, Teresa
Ferrer i Prats, Albert
author Chávez, Ángel
author_facet Chávez, Ángel
Castillo, Nídia
López-Tubau, Joan Manel
Atanasov, Kostadin Evgeniev
Fernández-Crespo, Emma
Camañes, Gemma
Altabella Artigas, Teresa
Ferrer i Prats, Albert
author_role author
author2 Castillo, Nídia
López-Tubau, Joan Manel
Atanasov, Kostadin Evgeniev
Fernández-Crespo, Emma
Camañes, Gemma
Altabella Artigas, Teresa
Ferrer i Prats, Albert
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Tomàquets
Arabidopsis
Glucòsids
Tomatoes
Arabidopsis
Glucosides
topic Tomàquets
Arabidopsis
Glucòsids
Tomatoes
Arabidopsis
Glucosides
description Free and glycosylated sterols localize in the plant cell plasma membrane, where in combination with other lipids regulate its structure and function. The role of glycosylated sterols in regulating membrane-associated biological processes is more relevant in plants like tomato (Solanum lycopersicum), in which glycosylated sterols are the predominant sterols. A proper ratio of free sterols versus glycosylated sterols has proven to be essential for proper plant performance in several species, but almost nothing is known in tomato. To assess the role of glycosylated sterols in tomato plant and fruit development, we generated transgenic lines of tomato cultivar Micro-Tom expressing two different amiRNAs devised to silence STEROL GLYCOSYLTRANSFERASE 1, the most actively expressed of the four genes encoding sterol glycosyltransferases in this plant. STEROL GLYCOSYLTRANSFERASE 1 gene silencing caused moderate plant dwarfism and reduced fruit size. Analysis of the profile of glycosylated sterols throughout fruit development demonstrated that the maintenance of proper levels of these compounds during the early stages of fruit development is essential for normal fruit growth, since reduced levels of glycosylated sterols trigger a transcriptional downregulatory response that affects genes involved in processes that are critical for proper fruit development, such as seed filling, cell wall extension and auxin signaling.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/193496
url https://hdl.handle.net/2445/193496
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.envexpbot.2022.105181
Environmental and Experimental Botany, 2022, vol. 206, num. 105181
https://doi.org/10.1016/j.envexpbot.2022.105181
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Ángel Chávez, et al, 2022
https://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Ángel Chávez, et al, 2022
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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