Constitutive expression of transgenes encoding derivatives of the synthetic antimicrobial peptide BP100: impact on rice host plant fitness

The Biopeptide BP100 is a synthetic and strongly cationic α-helical undecapeptide with high, specific antibacterial activity against economically important plant-pathogenic bacteria, and very low toxicity. It was selected from a library of synthetic peptides, along with other peptides with activitie...

Descripción completa

Detalles Bibliográficos
Autores: Nadal i Matamala, Anna, Montero Mirabet, Maria, Company Casadevall, Núria, Badosa Romañó, Esther, Messeguer i Peypoch, Joaquima, Montesinos Barreda, Laura, Montesinos Seguí, Emilio, Pla i de Solà-Morales, Maria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/7547
Acceso en línea:http://hdl.handle.net/10256/7547
Access Level:acceso abierto
Palabra clave:Antibiòtics pèptids
Peptide antibiotics
Plantes transgèniques
Transgenic plants
Arròs -- Genètica
Rice -- Genetics
id ES_3241a37ece2a4e42e9412904507d8bb7
oai_identifier_str oai:recercat.cat:10256/7547
network_acronym_str ES
network_name_str España
repository_id_str
spelling Constitutive expression of transgenes encoding derivatives of the synthetic antimicrobial peptide BP100: impact on rice host plant fitnessNadal i Matamala, AnnaMontero Mirabet, MariaCompany Casadevall, NúriaBadosa Romañó, EstherMesseguer i Peypoch, JoaquimaMontesinos Barreda, LauraMontesinos Seguí, EmilioPla i de Solà-Morales, MariaAntibiòtics pèptidsPeptide antibioticsPlantes transgèniquesTransgenic plantsArròs -- GenèticaRice -- GeneticsThe Biopeptide BP100 is a synthetic and strongly cationic α-helical undecapeptide with high, specific antibacterial activity against economically important plant-pathogenic bacteria, and very low toxicity. It was selected from a library of synthetic peptides, along with other peptides with activities against relevant bacterial and fungal species. Expression of the BP100 series of peptides in plants is of major interest to establish disease-resistant plants and facilitate molecular farming. Specific challenges were the small length, peptide degradation by plant proteases and toxicity to the host plant. Here we approached the expression of the BP100 peptide series in plants using BP100 as a proof-of-concept. Results: Our design considered up to three tandemly arranged BP100 units and peptide accumulation in the endoplasmic reticulum (ER), analyzing five BP100 derivatives. The ER retention sequence did not reduce the antimicrobial activity of chemically synthesized BP100 derivatives, making this strategy possible. Transformation with sequences encoding BP100 derivatives (bp100der) was over ten-fold less efficient than that of the hygromycin phosphotransferase (hptII) transgene. The BP100 direct tandems did not show higher antimicrobial activity than BP100, and genetically modified (GM) plants constitutively expressing them were not viable. In contrast, inverted repeats of BP100, whether or not elongated with a portion of a natural antimicrobial peptide (AMP), had higher antimicrobial activity, and fertile GM rice lines constitutively expressing bp100der were produced. These GM lines had increased resistance to the pathogens Dickeya chrysanthemi and Fusarium verticillioides, and tolerance to oxidative stress, with agronomic performance comparable to untransformed lines. Conclusions: Constitutive expression of transgenes encoding short cationic α-helical synthetic peptides can have a strong negative impact on rice fitness. However, GM plants expressing, for example, BP100 based on inverted repeats, have adequate agronomic performance and resistant phenotypes as a result of a complex equilibrium between bp100der toxicity to plant cells, antimicrobial activity and transgene-derived plant stress response. It is likely that these results can be extended to other peptides with similar characteristicsThis work was financially supported by the Spanish Ministerio de Ciencia e Innovacion (projects AGL2010-17181/AGR and PLANT-KBBE EUI2008-03769) and the Generalitat de Catalunya (SGR-2009-12)BioMed CentralMinisterio de Ciencia e Innovación (Espanya)2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10256/7547BMC Plant Biology, 2012, vol. 12, p. 159-180Articles publicats (D-EQATA)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2229-12-159info:eu-repo/semantics/altIdentifier/issn/1471-2229info:eu-repo/semantics/altIdentifier/eissn/1471-2229info:eu-repo/grantAgreement/MICINN//AGL2010-17181Attribution 2.0 Spainhttp://creativecommons.org/licenses/by/2.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:10256/75472026-05-29T05:05:01Z
dc.title.none.fl_str_mv Constitutive expression of transgenes encoding derivatives of the synthetic antimicrobial peptide BP100: impact on rice host plant fitness
title Constitutive expression of transgenes encoding derivatives of the synthetic antimicrobial peptide BP100: impact on rice host plant fitness
spellingShingle Constitutive expression of transgenes encoding derivatives of the synthetic antimicrobial peptide BP100: impact on rice host plant fitness
Nadal i Matamala, Anna
Antibiòtics pèptids
Peptide antibiotics
Plantes transgèniques
Transgenic plants
Arròs -- Genètica
Rice -- Genetics
title_short Constitutive expression of transgenes encoding derivatives of the synthetic antimicrobial peptide BP100: impact on rice host plant fitness
title_full Constitutive expression of transgenes encoding derivatives of the synthetic antimicrobial peptide BP100: impact on rice host plant fitness
title_fullStr Constitutive expression of transgenes encoding derivatives of the synthetic antimicrobial peptide BP100: impact on rice host plant fitness
title_full_unstemmed Constitutive expression of transgenes encoding derivatives of the synthetic antimicrobial peptide BP100: impact on rice host plant fitness
title_sort Constitutive expression of transgenes encoding derivatives of the synthetic antimicrobial peptide BP100: impact on rice host plant fitness
dc.creator.none.fl_str_mv Nadal i Matamala, Anna
Montero Mirabet, Maria
Company Casadevall, Núria
Badosa Romañó, Esther
Messeguer i Peypoch, Joaquima
Montesinos Barreda, Laura
Montesinos Seguí, Emilio
Pla i de Solà-Morales, Maria
author Nadal i Matamala, Anna
author_facet Nadal i Matamala, Anna
Montero Mirabet, Maria
Company Casadevall, Núria
Badosa Romañó, Esther
Messeguer i Peypoch, Joaquima
Montesinos Barreda, Laura
Montesinos Seguí, Emilio
Pla i de Solà-Morales, Maria
author_role author
author2 Montero Mirabet, Maria
Company Casadevall, Núria
Badosa Romañó, Esther
Messeguer i Peypoch, Joaquima
Montesinos Barreda, Laura
Montesinos Seguí, Emilio
Pla i de Solà-Morales, Maria
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (Espanya)
dc.subject.none.fl_str_mv Antibiòtics pèptids
Peptide antibiotics
Plantes transgèniques
Transgenic plants
Arròs -- Genètica
Rice -- Genetics
topic Antibiòtics pèptids
Peptide antibiotics
Plantes transgèniques
Transgenic plants
Arròs -- Genètica
Rice -- Genetics
description The Biopeptide BP100 is a synthetic and strongly cationic α-helical undecapeptide with high, specific antibacterial activity against economically important plant-pathogenic bacteria, and very low toxicity. It was selected from a library of synthetic peptides, along with other peptides with activities against relevant bacterial and fungal species. Expression of the BP100 series of peptides in plants is of major interest to establish disease-resistant plants and facilitate molecular farming. Specific challenges were the small length, peptide degradation by plant proteases and toxicity to the host plant. Here we approached the expression of the BP100 peptide series in plants using BP100 as a proof-of-concept. Results: Our design considered up to three tandemly arranged BP100 units and peptide accumulation in the endoplasmic reticulum (ER), analyzing five BP100 derivatives. The ER retention sequence did not reduce the antimicrobial activity of chemically synthesized BP100 derivatives, making this strategy possible. Transformation with sequences encoding BP100 derivatives (bp100der) was over ten-fold less efficient than that of the hygromycin phosphotransferase (hptII) transgene. The BP100 direct tandems did not show higher antimicrobial activity than BP100, and genetically modified (GM) plants constitutively expressing them were not viable. In contrast, inverted repeats of BP100, whether or not elongated with a portion of a natural antimicrobial peptide (AMP), had higher antimicrobial activity, and fertile GM rice lines constitutively expressing bp100der were produced. These GM lines had increased resistance to the pathogens Dickeya chrysanthemi and Fusarium verticillioides, and tolerance to oxidative stress, with agronomic performance comparable to untransformed lines. Conclusions: Constitutive expression of transgenes encoding short cationic α-helical synthetic peptides can have a strong negative impact on rice fitness. However, GM plants expressing, for example, BP100 based on inverted repeats, have adequate agronomic performance and resistant phenotypes as a result of a complex equilibrium between bp100der toxicity to plant cells, antimicrobial activity and transgene-derived plant stress response. It is likely that these results can be extended to other peptides with similar characteristics
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/7547
url http://hdl.handle.net/10256/7547
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2229-12-159
info:eu-repo/semantics/altIdentifier/issn/1471-2229
info:eu-repo/semantics/altIdentifier/eissn/1471-2229
info:eu-repo/grantAgreement/MICINN//AGL2010-17181
dc.rights.none.fl_str_mv Attribution 2.0 Spain
http://creativecommons.org/licenses/by/2.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 2.0 Spain
http://creativecommons.org/licenses/by/2.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv BMC Plant Biology, 2012, vol. 12, p. 159-180
Articles publicats (D-EQATA)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405663844630528
score 15.811543

Similares en LA Referencia