The woodchuck interferon-alpha system: Cloning, family description, and biologic activity
Interferon-alpha (IFN-alpha) is a key element in the defense against viral infection because, in addition to a direct antiviral effect, it exhibits potent immunostimulatory activity. To investigate the function of this cytokine in the woodchuck model of chronic hepatitis B, the woodchuck IFN-alpha g...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2002 |
| País: | España |
| Institución: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/23233 |
| Acceso en línea: | https://hdl.handle.net/10171/23233 |
| Access Level: | acceso abierto |
| Palabra clave: | Antiviral Viral hepatitis 2–5- oligoadenylate synthetase |
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The woodchuck interferon-alpha system: Cloning, family description, and biologic activityBerraondo-López, P. (Pedro)|||/items/b1f8ccc3-8e08-4ece-967c-64ccfc0e5b91Garcia-Navarro, R. (Raquel)|||/items/9c09be64-f0be-4416-9a09-f79c70d15934González-Aseguinolaza, G. (Gloria)|||/items/cb28732d-02bf-4339-ab60-ff738ee191acVales, A. (África)|||/items/f3b4e7dd-a2f9-4e77-9562-32b512a3772aBlanco-Urgoiti, B. (Begoña)|||/items/2728c716-9ca1-401e-9f5a-20fb7915c856Larrea-Leoz, E. (Esther)|||/items/37a0521f-3293-4001-85d1-bc71241d8a83Riezu-Boj, J.I. (José Ignacio)|||/items/87349e89-7360-4cdd-81be-2e8a6174b5f6Prieto, J. (Jesús)|||/items/0d9c3dec-4a09-400d-8c83-23ece1096c71Ruiz, J. (Juan)|||/items/e71ee758-aef7-4ed6-b282-e483e9d9ba8eAntiviralViral hepatitis2–5- oligoadenylate synthetaseInterferon-alpha (IFN-alpha) is a key element in the defense against viral infection because, in addition to a direct antiviral effect, it exhibits potent immunostimulatory activity. To investigate the function of this cytokine in the woodchuck model of chronic hepatitis B, the woodchuck IFN-alpha gene (IFNA) family was cloned and examined. The data indicate that this is a multigenic family from which 12 IFNA functional sequences and four pseudogene sequences were isolated. The overall identity of the amino acid sequence among the members of the woodchuck IFN-alpha family is 85%, and the identity with the IFN-alpha family from other species such as mice and humans is 50%. The analysis of hepatic expression of IFNA genes showed that wIFNA5a was the subtype transcribed preferentially in the woodchuck liver. The wIFNA genes transcribed in the liver were tested in an eukaryotic expression system and were found to enhance 2-5-oligoadenylate synthetase (2-5-OAS) mRNA levels and to posses a potent antiviral activity. Cloning of woodchuck IFNA genes will allow testing diverse forms of IFN-alpha delivery as well as different combination therapies in woodchuck hepatitis virus infection, thus providing useful information for the design of new strategies for the treatment of patients with chronic hepatitis B.Wiley-BlackwellDadun. Depósito Académico Digital Universidad de Navarra20122012-09-2620022002-01-0120022002-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/23233reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/232332026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
The woodchuck interferon-alpha system: Cloning, family description, and biologic activity |
| title |
The woodchuck interferon-alpha system: Cloning, family description, and biologic activity |
| spellingShingle |
The woodchuck interferon-alpha system: Cloning, family description, and biologic activity Berraondo-López, P. (Pedro)|||/items/b1f8ccc3-8e08-4ece-967c-64ccfc0e5b91 Antiviral Viral hepatitis 2–5- oligoadenylate synthetase |
| title_short |
The woodchuck interferon-alpha system: Cloning, family description, and biologic activity |
| title_full |
The woodchuck interferon-alpha system: Cloning, family description, and biologic activity |
| title_fullStr |
The woodchuck interferon-alpha system: Cloning, family description, and biologic activity |
| title_full_unstemmed |
The woodchuck interferon-alpha system: Cloning, family description, and biologic activity |
| title_sort |
The woodchuck interferon-alpha system: Cloning, family description, and biologic activity |
| dc.creator.none.fl_str_mv |
Berraondo-López, P. (Pedro)|||/items/b1f8ccc3-8e08-4ece-967c-64ccfc0e5b91 Garcia-Navarro, R. (Raquel)|||/items/9c09be64-f0be-4416-9a09-f79c70d15934 González-Aseguinolaza, G. (Gloria)|||/items/cb28732d-02bf-4339-ab60-ff738ee191ac Vales, A. (África)|||/items/f3b4e7dd-a2f9-4e77-9562-32b512a3772a Blanco-Urgoiti, B. (Begoña)|||/items/2728c716-9ca1-401e-9f5a-20fb7915c856 Larrea-Leoz, E. (Esther)|||/items/37a0521f-3293-4001-85d1-bc71241d8a83 Riezu-Boj, J.I. (José Ignacio)|||/items/87349e89-7360-4cdd-81be-2e8a6174b5f6 Prieto, J. (Jesús)|||/items/0d9c3dec-4a09-400d-8c83-23ece1096c71 Ruiz, J. (Juan)|||/items/e71ee758-aef7-4ed6-b282-e483e9d9ba8e |
| author |
Berraondo-López, P. (Pedro)|||/items/b1f8ccc3-8e08-4ece-967c-64ccfc0e5b91 |
| author_facet |
Berraondo-López, P. (Pedro)|||/items/b1f8ccc3-8e08-4ece-967c-64ccfc0e5b91 Garcia-Navarro, R. (Raquel)|||/items/9c09be64-f0be-4416-9a09-f79c70d15934 González-Aseguinolaza, G. (Gloria)|||/items/cb28732d-02bf-4339-ab60-ff738ee191ac Vales, A. (África)|||/items/f3b4e7dd-a2f9-4e77-9562-32b512a3772a Blanco-Urgoiti, B. (Begoña)|||/items/2728c716-9ca1-401e-9f5a-20fb7915c856 Larrea-Leoz, E. (Esther)|||/items/37a0521f-3293-4001-85d1-bc71241d8a83 Riezu-Boj, J.I. (José Ignacio)|||/items/87349e89-7360-4cdd-81be-2e8a6174b5f6 Prieto, J. (Jesús)|||/items/0d9c3dec-4a09-400d-8c83-23ece1096c71 Ruiz, J. (Juan)|||/items/e71ee758-aef7-4ed6-b282-e483e9d9ba8e |
| author_role |
author |
| author2 |
Garcia-Navarro, R. (Raquel)|||/items/9c09be64-f0be-4416-9a09-f79c70d15934 González-Aseguinolaza, G. (Gloria)|||/items/cb28732d-02bf-4339-ab60-ff738ee191ac Vales, A. (África)|||/items/f3b4e7dd-a2f9-4e77-9562-32b512a3772a Blanco-Urgoiti, B. (Begoña)|||/items/2728c716-9ca1-401e-9f5a-20fb7915c856 Larrea-Leoz, E. (Esther)|||/items/37a0521f-3293-4001-85d1-bc71241d8a83 Riezu-Boj, J.I. (José Ignacio)|||/items/87349e89-7360-4cdd-81be-2e8a6174b5f6 Prieto, J. (Jesús)|||/items/0d9c3dec-4a09-400d-8c83-23ece1096c71 Ruiz, J. (Juan)|||/items/e71ee758-aef7-4ed6-b282-e483e9d9ba8e |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Antiviral Viral hepatitis 2–5- oligoadenylate synthetase |
| topic |
Antiviral Viral hepatitis 2–5- oligoadenylate synthetase |
| description |
Interferon-alpha (IFN-alpha) is a key element in the defense against viral infection because, in addition to a direct antiviral effect, it exhibits potent immunostimulatory activity. To investigate the function of this cytokine in the woodchuck model of chronic hepatitis B, the woodchuck IFN-alpha gene (IFNA) family was cloned and examined. The data indicate that this is a multigenic family from which 12 IFNA functional sequences and four pseudogene sequences were isolated. The overall identity of the amino acid sequence among the members of the woodchuck IFN-alpha family is 85%, and the identity with the IFN-alpha family from other species such as mice and humans is 50%. The analysis of hepatic expression of IFNA genes showed that wIFNA5a was the subtype transcribed preferentially in the woodchuck liver. The wIFNA genes transcribed in the liver were tested in an eukaryotic expression system and were found to enhance 2-5-oligoadenylate synthetase (2-5-OAS) mRNA levels and to posses a potent antiviral activity. Cloning of woodchuck IFNA genes will allow testing diverse forms of IFN-alpha delivery as well as different combination therapies in woodchuck hepatitis virus infection, thus providing useful information for the design of new strategies for the treatment of patients with chronic hepatitis B. |
| publishDate |
2002 |
| dc.date.none.fl_str_mv |
2002 2002-01-01 2002 2002-01-01 2012 2012-09-26 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/23233 |
| url |
https://hdl.handle.net/10171/23233 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
| publisher.none.fl_str_mv |
Wiley-Blackwell |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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1869405660285763584 |
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15,301603 |