Fluorescence lifetime nanothermometer based on the equilibrium formation of anthracene AIE-excimers in living cells

The effective measurement of temperature in living systems at the nano and microscopic scales continues to be a challenge to this day. Here, we study the use of 2-(anthracen-2-yl)-1,3-diisopropylguanidine, 1, as a nanothermometer based on fluorescence lifetime measurements and its bioimaging applica...

ver descrição completa

Detalhes bibliográficos
Autores: Ripoll Lorente, María Consuelo, Campo Balguerías, Almudena del, Alonso Moreno, Carlos, Herrera Ochoa, Diego, Ocaña, Alberto, Martín Álvarez, Cristina, Garzón Ruiz, Andrés, Bravo Pérez, Iván
Tipo de documento: artigo
Data de publicação:2024
País:España
Recursos:Universidad de Castilla-La Mancha
Repositório:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/41565
Acesso em linha:https://doi.org/10.1016/j.jcis.2024.06.157
https://hdl.handle.net/10578/41565
Access Level:Acceso aberto
Palavra-chave:Aggregation Induced Emission
Anthracene Aggregates
Breast Cancer
Fluorescence Lifetime Imaging Microscopy
Living Systems
Nanothermometer
id ES_32127db2732e1b8bdf793c1f0dfd4ba4
oai_identifier_str oai:ruidera.uclm.es:10578/41565
network_acronym_str ES
network_name_str España
repository_id_str
spelling Fluorescence lifetime nanothermometer based on the equilibrium formation of anthracene AIE-excimers in living cellsRipoll Lorente, María ConsueloCampo Balguerías, Almudena delAlonso Moreno, CarlosHerrera Ochoa, DiegoOcaña, AlbertoMartín Álvarez, CristinaGarzón Ruiz, AndrésBravo Pérez, IvánAggregation Induced EmissionAnthracene AggregatesBreast CancerFluorescence Lifetime Imaging MicroscopyLiving SystemsNanothermometerThe effective measurement of temperature in living systems at the nano and microscopic scales continues to be a challenge to this day. Here, we study the use of 2-(anthracen-2-yl)-1,3-diisopropylguanidine, 1, as a nanothermometer based on fluorescence lifetime measurements and its bioimaging applications. In aqueous solution, 1 is shown in aggregated form and the equilibrium between the two main aggregate types (T-shaped and p-p) is highly sensitive to the temperature. The heating of the medium shifts the equilibrium toward the formation of highly emissive T-shaped aggregates. This species shows a high fluorescence emission and a long lifetime in comparison with the p-p aggregates and the freé monomer. A linear relationship between the fluorescence lifetime and the temperature both in aqueous solution and in a synthetic intracellular buffer was found. Fluorescence lifetime imaging microscopy (FLIM) also showed a linear relationship between lifetime and temperature with an excellent sensitivity in MCF7 breast cancer cells, which opens the door for its potential use as FLIM nanothermometer in the biomedical field.Elsevier202520252024info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://doi.org/10.1016/j.jcis.2024.06.157https://hdl.handle.net/10578/41565reponame:RUIdeRA. Repositorio Institucional de la UCLMinstname:Universidad de Castilla-La ManchaInglésPID2020-117788RB-I00RED2018-102387-TSBPLY/21/180501/0000502022-GRIN-341432019-GRIN-27175Predoctoral fellowship 2019/5964Postdoctoral fellowship 2021-UNIVERS-10414info:eu-repo/semantics/openAccessoai:ruidera.uclm.es:10578/415652026-05-27T07:36:41Z
dc.title.none.fl_str_mv Fluorescence lifetime nanothermometer based on the equilibrium formation of anthracene AIE-excimers in living cells
title Fluorescence lifetime nanothermometer based on the equilibrium formation of anthracene AIE-excimers in living cells
spellingShingle Fluorescence lifetime nanothermometer based on the equilibrium formation of anthracene AIE-excimers in living cells
Ripoll Lorente, María Consuelo
Aggregation Induced Emission
Anthracene Aggregates
Breast Cancer
Fluorescence Lifetime Imaging Microscopy
Living Systems
Nanothermometer
title_short Fluorescence lifetime nanothermometer based on the equilibrium formation of anthracene AIE-excimers in living cells
title_full Fluorescence lifetime nanothermometer based on the equilibrium formation of anthracene AIE-excimers in living cells
title_fullStr Fluorescence lifetime nanothermometer based on the equilibrium formation of anthracene AIE-excimers in living cells
title_full_unstemmed Fluorescence lifetime nanothermometer based on the equilibrium formation of anthracene AIE-excimers in living cells
title_sort Fluorescence lifetime nanothermometer based on the equilibrium formation of anthracene AIE-excimers in living cells
dc.creator.none.fl_str_mv Ripoll Lorente, María Consuelo
Campo Balguerías, Almudena del
Alonso Moreno, Carlos
Herrera Ochoa, Diego
Ocaña, Alberto
Martín Álvarez, Cristina
Garzón Ruiz, Andrés
Bravo Pérez, Iván
author Ripoll Lorente, María Consuelo
author_facet Ripoll Lorente, María Consuelo
Campo Balguerías, Almudena del
Alonso Moreno, Carlos
Herrera Ochoa, Diego
Ocaña, Alberto
Martín Álvarez, Cristina
Garzón Ruiz, Andrés
Bravo Pérez, Iván
author_role author
author2 Campo Balguerías, Almudena del
Alonso Moreno, Carlos
Herrera Ochoa, Diego
Ocaña, Alberto
Martín Álvarez, Cristina
Garzón Ruiz, Andrés
Bravo Pérez, Iván
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Aggregation Induced Emission
Anthracene Aggregates
Breast Cancer
Fluorescence Lifetime Imaging Microscopy
Living Systems
Nanothermometer
topic Aggregation Induced Emission
Anthracene Aggregates
Breast Cancer
Fluorescence Lifetime Imaging Microscopy
Living Systems
Nanothermometer
description The effective measurement of temperature in living systems at the nano and microscopic scales continues to be a challenge to this day. Here, we study the use of 2-(anthracen-2-yl)-1,3-diisopropylguanidine, 1, as a nanothermometer based on fluorescence lifetime measurements and its bioimaging applications. In aqueous solution, 1 is shown in aggregated form and the equilibrium between the two main aggregate types (T-shaped and p-p) is highly sensitive to the temperature. The heating of the medium shifts the equilibrium toward the formation of highly emissive T-shaped aggregates. This species shows a high fluorescence emission and a long lifetime in comparison with the p-p aggregates and the freé monomer. A linear relationship between the fluorescence lifetime and the temperature both in aqueous solution and in a synthetic intracellular buffer was found. Fluorescence lifetime imaging microscopy (FLIM) also showed a linear relationship between lifetime and temperature with an excellent sensitivity in MCF7 breast cancer cells, which opens the door for its potential use as FLIM nanothermometer in the biomedical field.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.jcis.2024.06.157
https://hdl.handle.net/10578/41565
url https://doi.org/10.1016/j.jcis.2024.06.157
https://hdl.handle.net/10578/41565
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PID2020-117788RB-I00
RED2018-102387-T
SBPLY/21/180501/000050
2022-GRIN-34143
2019-GRIN-27175
Predoctoral fellowship 2019/5964
Postdoctoral fellowship 2021-UNIVERS-10414
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:RUIdeRA. Repositorio Institucional de la UCLM
instname:Universidad de Castilla-La Mancha
instname_str Universidad de Castilla-La Mancha
reponame_str RUIdeRA. Repositorio Institucional de la UCLM
collection RUIdeRA. Repositorio Institucional de la UCLM
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405652234797056
score 15,811543

Registros relacionados