Modulation in Wistar rats of blood corticosterone compartmentation by sex and prior exposure to a cafeteria diet

In the metabolic syndrome, glucocorticoid activity is increased, but circulating levels show little change. Most of blood glucocorticoids are bound to corticosteroid-binding globulin (CBG), which liver expression and circulating levels are higher in females than in males. Since blood hormones are al...

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Autores: Romero Romero, María del Mar, Holmgren-Holm, Fredrik, Grasa Martínez, Maria del Mar, Esteve Ràfols, Montserrat, Remesar Betlloch, Xavier, Fernández López, José Antonio, Alemany, Marià, 1946-
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/53114
Acceso en línea:https://hdl.handle.net/2445/53114
Access Level:acceso abierto
Palabra clave:Dieta
Hormones esteroides
Corticosteroides
Diferències entre sexes
Rates (Animals de laboratori)
Diet
Steroid hormones
Adrenocortical hormones
Sex differences
Rats as laboratory animals
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spelling Modulation in Wistar rats of blood corticosterone compartmentation by sex and prior exposure to a cafeteria dietRomero Romero, María del MarHolmgren-Holm, FredrikGrasa Martínez, Maria del MarEsteve Ràfols, MontserratRemesar Betlloch, XavierFernández López, José AntonioAlemany, Marià, 1946-DietaHormones esteroidesCorticosteroidesDiferències entre sexesRates (Animals de laboratori)DietSteroid hormonesAdrenocortical hormonesSex differencesRats as laboratory animalsIn the metabolic syndrome, glucocorticoid activity is increased, but circulating levels show little change. Most of blood glucocorticoids are bound to corticosteroid-binding globulin (CBG), which liver expression and circulating levels are higher in females than in males. Since blood hormones are also bound to blood cells, and the size of this compartment is considerable for androgens and estrogens, we analyzed whether sex or eating a cafeteria diet altered the compartmentation of corticosterone in rat blood. The main corticosterone compartment in rat blood is that specifically bound to plasma proteins, with smaller compartments bound to blood cells or free. Cafeteria diet increased the expression of liver CBG gene, binding plasma capacity and the proportion of blood cell-bound corticosterone. There were marked sex differences in blood corticosterone compartmentation in rats, which were unrelated to testosterone. The use of a monoclonal antibody ELISA and a polyclonal Western blot for plasma CBG compared with both specific plasma binding of corticosterone and CBG gene expression suggested the existence of different forms of CBG, with varying affinities for corticosterone in males and females, since ELISA data showed higher plasma CBG for males, but binding and Western blot analyses (plus liver gene expression) and higher physiological effectiveness for females. Good cross- reactivity to the antigen for polyclonal CBG antibody suggests that in all cases we were measuring CBG.The different immunoreactivity and binding affinity may help explain the marked sex-related differences in plasma hormone binding as sex-linked different proportions of CBG forms.Public Library of Science (PLoS)2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/53114Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0057342PLoS One, 2013, vol. 8, num. 2, p. e57342http://dx.doi.org/10.1371/journal.pone.0057342cc-by (c) Romero Romero, María del Mar et al., 2013http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/531142026-05-27T06:46:51Z
dc.title.none.fl_str_mv Modulation in Wistar rats of blood corticosterone compartmentation by sex and prior exposure to a cafeteria diet
title Modulation in Wistar rats of blood corticosterone compartmentation by sex and prior exposure to a cafeteria diet
spellingShingle Modulation in Wistar rats of blood corticosterone compartmentation by sex and prior exposure to a cafeteria diet
Romero Romero, María del Mar
Dieta
Hormones esteroides
Corticosteroides
Diferències entre sexes
Rates (Animals de laboratori)
Diet
Steroid hormones
Adrenocortical hormones
Sex differences
Rats as laboratory animals
title_short Modulation in Wistar rats of blood corticosterone compartmentation by sex and prior exposure to a cafeteria diet
title_full Modulation in Wistar rats of blood corticosterone compartmentation by sex and prior exposure to a cafeteria diet
title_fullStr Modulation in Wistar rats of blood corticosterone compartmentation by sex and prior exposure to a cafeteria diet
title_full_unstemmed Modulation in Wistar rats of blood corticosterone compartmentation by sex and prior exposure to a cafeteria diet
title_sort Modulation in Wistar rats of blood corticosterone compartmentation by sex and prior exposure to a cafeteria diet
dc.creator.none.fl_str_mv Romero Romero, María del Mar
Holmgren-Holm, Fredrik
Grasa Martínez, Maria del Mar
Esteve Ràfols, Montserrat
Remesar Betlloch, Xavier
Fernández López, José Antonio
Alemany, Marià, 1946-
author Romero Romero, María del Mar
author_facet Romero Romero, María del Mar
Holmgren-Holm, Fredrik
Grasa Martínez, Maria del Mar
Esteve Ràfols, Montserrat
Remesar Betlloch, Xavier
Fernández López, José Antonio
Alemany, Marià, 1946-
author_role author
author2 Holmgren-Holm, Fredrik
Grasa Martínez, Maria del Mar
Esteve Ràfols, Montserrat
Remesar Betlloch, Xavier
Fernández López, José Antonio
Alemany, Marià, 1946-
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Dieta
Hormones esteroides
Corticosteroides
Diferències entre sexes
Rates (Animals de laboratori)
Diet
Steroid hormones
Adrenocortical hormones
Sex differences
Rats as laboratory animals
topic Dieta
Hormones esteroides
Corticosteroides
Diferències entre sexes
Rates (Animals de laboratori)
Diet
Steroid hormones
Adrenocortical hormones
Sex differences
Rats as laboratory animals
description In the metabolic syndrome, glucocorticoid activity is increased, but circulating levels show little change. Most of blood glucocorticoids are bound to corticosteroid-binding globulin (CBG), which liver expression and circulating levels are higher in females than in males. Since blood hormones are also bound to blood cells, and the size of this compartment is considerable for androgens and estrogens, we analyzed whether sex or eating a cafeteria diet altered the compartmentation of corticosterone in rat blood. The main corticosterone compartment in rat blood is that specifically bound to plasma proteins, with smaller compartments bound to blood cells or free. Cafeteria diet increased the expression of liver CBG gene, binding plasma capacity and the proportion of blood cell-bound corticosterone. There were marked sex differences in blood corticosterone compartmentation in rats, which were unrelated to testosterone. The use of a monoclonal antibody ELISA and a polyclonal Western blot for plasma CBG compared with both specific plasma binding of corticosterone and CBG gene expression suggested the existence of different forms of CBG, with varying affinities for corticosterone in males and females, since ELISA data showed higher plasma CBG for males, but binding and Western blot analyses (plus liver gene expression) and higher physiological effectiveness for females. Good cross- reactivity to the antigen for polyclonal CBG antibody suggests that in all cases we were measuring CBG.The different immunoreactivity and binding affinity may help explain the marked sex-related differences in plasma hormone binding as sex-linked different proportions of CBG forms.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/53114
url https://hdl.handle.net/2445/53114
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0057342
PLoS One, 2013, vol. 8, num. 2, p. e57342
http://dx.doi.org/10.1371/journal.pone.0057342
dc.rights.none.fl_str_mv cc-by (c) Romero Romero, María del Mar et al., 2013
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Romero Romero, María del Mar et al., 2013
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869405650927222784
score 15,301603

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