Cloning and expression analysis of two ROR-γ homologues (ROR-γa1 and ROR-γa2) in rainbow trout Oncorhynchus mykiss

10 páginas, 8 figuras, 1 tabla

Detalles Bibliográficos
Autores: Monte, Milena M., Wang, Tiehui, Costa, M. M., Harun, N. O., Secombes, Christopher J.
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/64698
Acceso en línea:http://hdl.handle.net/10261/64698
Access Level:acceso abierto
Palabra clave:Oncorhynchus mykiss
ROR-γ
Bacterial infection
Vaccination
Viral infection
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spelling Cloning and expression analysis of two ROR-γ homologues (ROR-γa1 and ROR-γa2) in rainbow trout Oncorhynchus mykissMonte, Milena M.Wang, TiehuiCosta, M. M.Harun, N. O.Secombes, Christopher J.Oncorhynchus mykissROR-γBacterial infectionVaccinationViral infection10 páginas, 8 figuras, 1 tablaThis paper describes the cloning and characterisation of two retinoid-related orphan receptor (ROR)-γ homologues (ROR-γa1 and -γa2) in rainbow trout (Oncorhynchus mykiss). The coding region predicted for both homologues consists of 1410 base pairs (bp), which translate into two 469 amino acid (aa) proteins. The trout ROR-γs revealed a high conservation of both DNA- and ligand-binding domains (functional regions of the nuclear receptor family), and shared a high homology to mammalian ROR-γt. A phylogenetic tree containing ROR family members confirmed that both trout homologues clustered within the ROR-γ group. Both results suggested that these molecules are likely to be ROR-γ homologues, more similar to the mammalian splice variant ROR-γt than the full length ROR-γ. Expression analysis of tissues obtained from healthy fish revealed highest constitutive expression of trout ROR-γ in muscle, followed by the brain, heart and skin. This suggests that these genes may play an important role in such tissues. In vitro studies, using trout cell lines, demonstrated that ROR-γ is induced significantly by LPS and down-regulated by the presence of PolyI:C and recombinant interferon (IFN)-γ. Moreover, analysis of this gene in head kidney macrophages and mixed primary leucocyte cultures indicated that differences were apparent between the different cell types/sources used, indicating that its expression may be cell-type dependent. Additional studies to investigate the regulation of this gene in vivo demonstrated that its expression was significantly higher in vaccinated vs unvaccinated fish following bacterial (Yersinia ruckeri) challenge but it was down-regulated after a viral (VHSV) infection. This suggests a potential role of trout ROR-γ, a putative TH17 transcription factor, in protection against extracellular bacteria.This work was supported financially by a PhD studentship from the FCT (Foundation for Science and Technology, Portugal) and POPH/FSE (Grant no. SFRH/BD/38236/2007) to Milena M. Monte, and the European Commission Contract No. 007103 (IMAQUANIM-Improved immunity of aquacultured animals). Maria M. Costa received funding from the Consejo Superior de Investigaciones Científicas (CSIC, Spain) and the Xunta de Galicia for her “Ángeles Alvariño” postdoctoral contract. Nor O. Harun received funding from the Ministry of Higher Education (Malaysia) and the University Malaysia Terengganu for her PhD studiesPeer reviewedElsevierConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201320132012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/64698reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.fsi.2012.05.023Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/646982026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cloning and expression analysis of two ROR-γ homologues (ROR-γa1 and ROR-γa2) in rainbow trout Oncorhynchus mykiss
title Cloning and expression analysis of two ROR-γ homologues (ROR-γa1 and ROR-γa2) in rainbow trout Oncorhynchus mykiss
spellingShingle Cloning and expression analysis of two ROR-γ homologues (ROR-γa1 and ROR-γa2) in rainbow trout Oncorhynchus mykiss
Monte, Milena M.
Oncorhynchus mykiss
ROR-γ
Bacterial infection
Vaccination
Viral infection
title_short Cloning and expression analysis of two ROR-γ homologues (ROR-γa1 and ROR-γa2) in rainbow trout Oncorhynchus mykiss
title_full Cloning and expression analysis of two ROR-γ homologues (ROR-γa1 and ROR-γa2) in rainbow trout Oncorhynchus mykiss
title_fullStr Cloning and expression analysis of two ROR-γ homologues (ROR-γa1 and ROR-γa2) in rainbow trout Oncorhynchus mykiss
title_full_unstemmed Cloning and expression analysis of two ROR-γ homologues (ROR-γa1 and ROR-γa2) in rainbow trout Oncorhynchus mykiss
title_sort Cloning and expression analysis of two ROR-γ homologues (ROR-γa1 and ROR-γa2) in rainbow trout Oncorhynchus mykiss
dc.creator.none.fl_str_mv Monte, Milena M.
Wang, Tiehui
Costa, M. M.
Harun, N. O.
Secombes, Christopher J.
author Monte, Milena M.
author_facet Monte, Milena M.
Wang, Tiehui
Costa, M. M.
Harun, N. O.
Secombes, Christopher J.
author_role author
author2 Wang, Tiehui
Costa, M. M.
Harun, N. O.
Secombes, Christopher J.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Oncorhynchus mykiss
ROR-γ
Bacterial infection
Vaccination
Viral infection
topic Oncorhynchus mykiss
ROR-γ
Bacterial infection
Vaccination
Viral infection
description 10 páginas, 8 figuras, 1 tabla
publishDate 2012
dc.date.none.fl_str_mv 2012
2013
2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/64698
url http://hdl.handle.net/10261/64698
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.fsi.2012.05.023
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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