In vivo testing of gold nanoparticles using the Caenorhabditis elegans model organism

Gold nanoparticles (AuNPs) are present in many man-made products and cosmetics, and are also used by the food and medical industries. Tight regulations regarding the use of mammalian animals for product testing can hamper the study of the specific interactions between engineered nanoparticles and bi...

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Autores: Gonzalez-Moragasa, Laura, Berto, Clara, Vilches, Clara, Quidant, Romain, Kolovouc, Androniki, Santarella-Mellwig, Rachel, Schwab, Yannick, Stürzenbaum, Stephen, Roig, Anna, Laromainea, Anna
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/100837
Acceso en línea:https://hdl.handle.net/2117/100837
Access Level:acceso abierto
Palabra clave:Caenorhabditis elegans
Àrees temàtiques de la UPC::Física
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spelling In vivo testing of gold nanoparticles using the Caenorhabditis elegans model organismGonzalez-Moragasa, LauraBerto, ClaraVilches, ClaraQuidant, RomainKolovouc, AndronikiSantarella-Mellwig, RachelSchwab, YannickStürzenbaum, StephenRoig, AnnaLaromainea, AnnaCaenorhabditis elegansCaenorhabditis elegansCaenorhabditis elegansÀrees temàtiques de la UPC::FísicaGold nanoparticles (AuNPs) are present in many man-made products and cosmetics, and are also used by the food and medical industries. Tight regulations regarding the use of mammalian animals for product testing can hamper the study of the specific interactions between engineered nanoparticles and biological systems. Invertebrate models, such as the nematode Caenorhabditis elegans (C. elegans), can offer alternative approaches during the early phases of nanoparticle discovery. Here, we thoroughly evaluated the biodistribution of 11-nm and 150-nm citrate-capped AuNPs in the model organism C. elegans at multiple scales, moving from micrometric to nanometric resolution and from the organismal to cellular level. We confirmed that the nanoparticles were not able to cross the intestinal and dermal barriers. We investigated the effect of AuNPs on the survival and reproductive performance of C. elegans, and correlated these effects with the uptake of AuNPs in terms of their number, surface area, and metal mass. In general, exposure to 11-nm AuNPs resulted in a higher toxicity than the larger 150-nm AuNPs. NP aggregation inside C. elegans was determined using absorbance microspectroscopy, which allowed the plasmonic properties of AuNPs to be correlated with their confinement inside the intestinal lumen, where anatomical traits, acidic pH and the presence of biomolecules play an essential role on NP aggregation. Finally, quantitative PCR of selected molecular markers indicated that exposure to AuNPs did not significantly affect endocytosis and intestinal barrier integrity. Statement of significance This work highlights how the simple, yet information-rich, animal model C. elegans is ideally suited for preliminary screening of nanoparticles or chemicals mitigating most of the difficulties associated with mammalian animal models, namely the ethical issues, the high cost, and time constraints. This is of particular relevance to the cosmetic, food, and pharmaceutical industries, which all have to justify the use of animals, especially during the discovery, development and initial screening phases. This work provides a detailed and thorough analysis of 11-nm and 150-nm AuNPs at multiple levels of organization (the whole organism, organs, tissues, cells and molecules).Peer ReviewedElsevier20172017-02-0120172017-02-10journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/100837reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)InglésengMinisterio de Economía y Competitividad http://doi.org/10.13039/501100003329 SEV-2015-0496 INSTITUTO DE CIENCIA DE MATERIALES DE BARCELONA ICMABEuropean Commission http://dx.doi.org/10.13039/100011102 Seventh Framework Programme 303630 Interactions in Three-dimensional (3D) in vitro environments of Nanoparticles and Cellsopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/1008372026-05-27T15:37:01Z
dc.title.none.fl_str_mv In vivo testing of gold nanoparticles using the Caenorhabditis elegans model organism
title In vivo testing of gold nanoparticles using the Caenorhabditis elegans model organism
spellingShingle In vivo testing of gold nanoparticles using the Caenorhabditis elegans model organism
Gonzalez-Moragasa, Laura
Caenorhabditis elegans
Caenorhabditis elegans
Caenorhabditis elegans
Àrees temàtiques de la UPC::Física
title_short In vivo testing of gold nanoparticles using the Caenorhabditis elegans model organism
title_full In vivo testing of gold nanoparticles using the Caenorhabditis elegans model organism
title_fullStr In vivo testing of gold nanoparticles using the Caenorhabditis elegans model organism
title_full_unstemmed In vivo testing of gold nanoparticles using the Caenorhabditis elegans model organism
title_sort In vivo testing of gold nanoparticles using the Caenorhabditis elegans model organism
dc.creator.none.fl_str_mv Gonzalez-Moragasa, Laura
Berto, Clara
Vilches, Clara
Quidant, Romain
Kolovouc, Androniki
Santarella-Mellwig, Rachel
Schwab, Yannick
Stürzenbaum, Stephen
Roig, Anna
Laromainea, Anna
author Gonzalez-Moragasa, Laura
author_facet Gonzalez-Moragasa, Laura
Berto, Clara
Vilches, Clara
Quidant, Romain
Kolovouc, Androniki
Santarella-Mellwig, Rachel
Schwab, Yannick
Stürzenbaum, Stephen
Roig, Anna
Laromainea, Anna
author_role author
author2 Berto, Clara
Vilches, Clara
Quidant, Romain
Kolovouc, Androniki
Santarella-Mellwig, Rachel
Schwab, Yannick
Stürzenbaum, Stephen
Roig, Anna
Laromainea, Anna
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Caenorhabditis elegans
Caenorhabditis elegans
Caenorhabditis elegans
Àrees temàtiques de la UPC::Física
topic Caenorhabditis elegans
Caenorhabditis elegans
Caenorhabditis elegans
Àrees temàtiques de la UPC::Física
description Gold nanoparticles (AuNPs) are present in many man-made products and cosmetics, and are also used by the food and medical industries. Tight regulations regarding the use of mammalian animals for product testing can hamper the study of the specific interactions between engineered nanoparticles and biological systems. Invertebrate models, such as the nematode Caenorhabditis elegans (C. elegans), can offer alternative approaches during the early phases of nanoparticle discovery. Here, we thoroughly evaluated the biodistribution of 11-nm and 150-nm citrate-capped AuNPs in the model organism C. elegans at multiple scales, moving from micrometric to nanometric resolution and from the organismal to cellular level. We confirmed that the nanoparticles were not able to cross the intestinal and dermal barriers. We investigated the effect of AuNPs on the survival and reproductive performance of C. elegans, and correlated these effects with the uptake of AuNPs in terms of their number, surface area, and metal mass. In general, exposure to 11-nm AuNPs resulted in a higher toxicity than the larger 150-nm AuNPs. NP aggregation inside C. elegans was determined using absorbance microspectroscopy, which allowed the plasmonic properties of AuNPs to be correlated with their confinement inside the intestinal lumen, where anatomical traits, acidic pH and the presence of biomolecules play an essential role on NP aggregation. Finally, quantitative PCR of selected molecular markers indicated that exposure to AuNPs did not significantly affect endocytosis and intestinal barrier integrity. Statement of significance This work highlights how the simple, yet information-rich, animal model C. elegans is ideally suited for preliminary screening of nanoparticles or chemicals mitigating most of the difficulties associated with mammalian animal models, namely the ethical issues, the high cost, and time constraints. This is of particular relevance to the cosmetic, food, and pharmaceutical industries, which all have to justify the use of animals, especially during the discovery, development and initial screening phases. This work provides a detailed and thorough analysis of 11-nm and 150-nm AuNPs at multiple levels of organization (the whole organism, organs, tissues, cells and molecules).
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-02-01
2017
2017-02-10
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/100837
url https://hdl.handle.net/2117/100837
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://doi.org/10.13039/501100003329 SEV-2015-0496 INSTITUTO DE CIENCIA DE MATERIALES DE BARCELONA ICMAB
European Commission http://dx.doi.org/10.13039/100011102 Seventh Framework Programme 303630 Interactions in Three-dimensional (3D) in vitro environments of Nanoparticles and Cells
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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