The Role of Testosterone in Spermatogenesis: lessons from proteome profiling of human spermatozoa in testosterone deficiency

Testosterone is essential to maintain qualitative spermatogenesis. Nonetheless, no studies have been yet performed in humans to analyze the testosterone-mediated expression of sperm proteins and their importance in reproduction. Thus, this study aimed to identify sperm protein alterations in male hy...

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Autores: Grande, Giuseppe, Barrachina, Ferran, Soler Ventura, Ada, Jodar Bifet, Meritxell, Mancini, Francesca, Marana, Riccardo, Chiloiro, Sabrina, Pontecorvi, Alfredo, Oliva Virgili, Rafael, Milardi, Domenico
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/191121
Acceso en línea:https://hdl.handle.net/2445/191121
Access Level:acceso abierto
Palabra clave:Espermatozoides
Testosterona
Proteòmica
Hipogonadisme
Hormona luteostimulant
Spermatozoa
Testosterone
Proteomics
Hypogonadism
Luteinizing hormone
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spelling The Role of Testosterone in Spermatogenesis: lessons from proteome profiling of human spermatozoa in testosterone deficiencyGrande, GiuseppeBarrachina, FerranSoler Ventura, AdaJodar Bifet, MeritxellMancini, FrancescaMarana, RiccardoChiloiro, SabrinaPontecorvi, AlfredoOliva Virgili, RafaelMilardi, DomenicoEspermatozoidesTestosteronaProteòmicaHipogonadismeHormona luteostimulantSpermatozoaTestosteroneProteomicsHypogonadismLuteinizing hormoneTestosterone is essential to maintain qualitative spermatogenesis. Nonetheless, no studies have been yet performed in humans to analyze the testosterone-mediated expression of sperm proteins and their importance in reproduction. Thus, this study aimed to identify sperm protein alterations in male hypogonadism using proteomic profiling. We have performed a comparative proteomic analysis comparing sperm from fertile controls (a pool of 5 normogonadic normozoospermic fertile men) versus sperm from patients with secondary hypogonadism (a pool of 5 oligozoospermic hypogonadic patients due to isolated LH deficiency). Sperm protein composition was analyzed, after peptide labelling with Isobaric Tags, via liquid chromatography followed by tandem mass spectrometry (LC-MS/MS) on an LTQ Velos-Orbitrap mass spectrometer. LC-MS/MS data were analyzed using Proteome Discoverer. Criteria used to accept protein identification included a false discovery rate (FDR) of 1% and at least 1 peptide match per protein. Up to 986 proteins were identified and, of those, 43 proteins were differentially expressed: 32 proteins were under-expressed and 11 were over-expressed in the pool of hypogonadic patients compared to the controls. Bioinformatic analyses were performed using UniProt Knowledgebase, and the Gene Ontology Consortium database based on PANTHER. Notably, 13 of these 43 differentially expressed proteins have been previously reported to be related to sperm function and spermatogenesis. Western blot analyses for A-Kinase Anchoring Protein 3 (AKAP3) and the Prolactin Inducible Protein (PIP) were used to confirm the proteomics data. In summary, a high-resolution mass spectrometry-based proteomic approach was used for the first time to describe alterations of the sperm proteome in secondary male hypogonadism. Some of the differential sperm proteins described in this study, which include Prosaposin, SMOC-1, SERPINA5, SPANXB1, GSG1, ELSPBP1, fibronectin, 5-oxoprolinase, AKAP3, AKAP4, HYDIN, ROPN1B, ß-Microseminoprotein and Protein S100-A8, could represent new targets for the design of infertility treatments due to androgen deficiency.Frontiers Media2022202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion11 p.application/pdfhttps://hdl.handle.net/2445/191121Articles publicats en revistes (Biomedicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3389/fendo.2022.852661Frontiers In Endocrinology, 2022, vol. 13, p. 852661https://doi.org/10.3389/fendo.2022.852661cc-by (c) Grande, Giuseppe et al., 2022https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1911212026-05-29T05:05:01Z
dc.title.none.fl_str_mv The Role of Testosterone in Spermatogenesis: lessons from proteome profiling of human spermatozoa in testosterone deficiency
title The Role of Testosterone in Spermatogenesis: lessons from proteome profiling of human spermatozoa in testosterone deficiency
spellingShingle The Role of Testosterone in Spermatogenesis: lessons from proteome profiling of human spermatozoa in testosterone deficiency
Grande, Giuseppe
Espermatozoides
Testosterona
Proteòmica
Hipogonadisme
Hormona luteostimulant
Spermatozoa
Testosterone
Proteomics
Hypogonadism
Luteinizing hormone
title_short The Role of Testosterone in Spermatogenesis: lessons from proteome profiling of human spermatozoa in testosterone deficiency
title_full The Role of Testosterone in Spermatogenesis: lessons from proteome profiling of human spermatozoa in testosterone deficiency
title_fullStr The Role of Testosterone in Spermatogenesis: lessons from proteome profiling of human spermatozoa in testosterone deficiency
title_full_unstemmed The Role of Testosterone in Spermatogenesis: lessons from proteome profiling of human spermatozoa in testosterone deficiency
title_sort The Role of Testosterone in Spermatogenesis: lessons from proteome profiling of human spermatozoa in testosterone deficiency
dc.creator.none.fl_str_mv Grande, Giuseppe
Barrachina, Ferran
Soler Ventura, Ada
Jodar Bifet, Meritxell
Mancini, Francesca
Marana, Riccardo
Chiloiro, Sabrina
Pontecorvi, Alfredo
Oliva Virgili, Rafael
Milardi, Domenico
author Grande, Giuseppe
author_facet Grande, Giuseppe
Barrachina, Ferran
Soler Ventura, Ada
Jodar Bifet, Meritxell
Mancini, Francesca
Marana, Riccardo
Chiloiro, Sabrina
Pontecorvi, Alfredo
Oliva Virgili, Rafael
Milardi, Domenico
author_role author
author2 Barrachina, Ferran
Soler Ventura, Ada
Jodar Bifet, Meritxell
Mancini, Francesca
Marana, Riccardo
Chiloiro, Sabrina
Pontecorvi, Alfredo
Oliva Virgili, Rafael
Milardi, Domenico
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Espermatozoides
Testosterona
Proteòmica
Hipogonadisme
Hormona luteostimulant
Spermatozoa
Testosterone
Proteomics
Hypogonadism
Luteinizing hormone
topic Espermatozoides
Testosterona
Proteòmica
Hipogonadisme
Hormona luteostimulant
Spermatozoa
Testosterone
Proteomics
Hypogonadism
Luteinizing hormone
description Testosterone is essential to maintain qualitative spermatogenesis. Nonetheless, no studies have been yet performed in humans to analyze the testosterone-mediated expression of sperm proteins and their importance in reproduction. Thus, this study aimed to identify sperm protein alterations in male hypogonadism using proteomic profiling. We have performed a comparative proteomic analysis comparing sperm from fertile controls (a pool of 5 normogonadic normozoospermic fertile men) versus sperm from patients with secondary hypogonadism (a pool of 5 oligozoospermic hypogonadic patients due to isolated LH deficiency). Sperm protein composition was analyzed, after peptide labelling with Isobaric Tags, via liquid chromatography followed by tandem mass spectrometry (LC-MS/MS) on an LTQ Velos-Orbitrap mass spectrometer. LC-MS/MS data were analyzed using Proteome Discoverer. Criteria used to accept protein identification included a false discovery rate (FDR) of 1% and at least 1 peptide match per protein. Up to 986 proteins were identified and, of those, 43 proteins were differentially expressed: 32 proteins were under-expressed and 11 were over-expressed in the pool of hypogonadic patients compared to the controls. Bioinformatic analyses were performed using UniProt Knowledgebase, and the Gene Ontology Consortium database based on PANTHER. Notably, 13 of these 43 differentially expressed proteins have been previously reported to be related to sperm function and spermatogenesis. Western blot analyses for A-Kinase Anchoring Protein 3 (AKAP3) and the Prolactin Inducible Protein (PIP) were used to confirm the proteomics data. In summary, a high-resolution mass spectrometry-based proteomic approach was used for the first time to describe alterations of the sperm proteome in secondary male hypogonadism. Some of the differential sperm proteins described in this study, which include Prosaposin, SMOC-1, SERPINA5, SPANXB1, GSG1, ELSPBP1, fibronectin, 5-oxoprolinase, AKAP3, AKAP4, HYDIN, ROPN1B, ß-Microseminoprotein and Protein S100-A8, could represent new targets for the design of infertility treatments due to androgen deficiency.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/191121
url https://hdl.handle.net/2445/191121
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fendo.2022.852661
Frontiers In Endocrinology, 2022, vol. 13, p. 852661
https://doi.org/10.3389/fendo.2022.852661
dc.rights.none.fl_str_mv cc-by (c) Grande, Giuseppe et al., 2022
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Grande, Giuseppe et al., 2022
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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