Determination of Methotrexate in pH-Sensitive Chitosan Nanoparticles by Validated RP-LC and UV Spectrophotometric Methods
Nanotechnology-based drug delivery systems are in constant development and, therefore, it is of great importance to have rapid, efficient and accurate analytical methodology to quantify the encapsulated drugs. Here, simple and fast methods, by reversed-phase liquid chromatography (RP-LC) and UV spec...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/97209 |
| Acceso en línea: | https://hdl.handle.net/2445/97209 |
| Access Level: | acceso abierto |
| Palabra clave: | Quitosan Nanopartícules Medicaments antineoplàstics Metotrexat Agents tensioactius Cromatografia Espectroscòpia Chitosan Nanoparticles Antineoplastic agents Methotrexate Surface active agents Chromatography Spectrum analysis |
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Determination of Methotrexate in pH-Sensitive Chitosan Nanoparticles by Validated RP-LC and UV Spectrophotometric MethodsNogueira, Daniele R.Macedo, Letícia B.Scheeren, Laís E.Mitjans Arnal, MontserratInfante Martínez-Pardo, Ma. RosaRolim, Clarice M. B.Vinardell Martínez-Hidalgo, Ma. PilarQuitosanNanopartículesMedicaments antineoplàsticsMetotrexatAgents tensioactiusCromatografiaEspectroscòpiaChitosanNanoparticlesAntineoplastic agentsMethotrexateSurface active agentsChromatographySpectrum analysisNanotechnology-based drug delivery systems are in constant development and, therefore, it is of great importance to have rapid, efficient and accurate analytical methodology to quantify the encapsulated drugs. Here, simple and fast methods, by reversed-phase liquid chromatography (RP-LC) and UV spectrophotometry, were developed and validated for the determination of methotrexate (MTX) in pH-sensitive chitosan nanoparticles (CS-NPs). NPs were prepared using a modified ionotropic complexation process, in which was included a surfactant derived from Nα,Nε-dioctanoyl lysine with an inorganic sodium counterion. The RP-LC method was carried out on a Waters XBridgeTM C18 column (250 mm x 4.6 mm I.D., 5μm), with mobile phase consisted of potassium phosphate buffer (0.05 M, pH 3.2): acetonitrile (86:14, v/v), and UV detection set at 303 nm. The analyses of MTX content by the UV method were also accomplished at 303 nm, using 0.1 M sodium hydroxide as diluent. The measurements were linearly correlated with concentration for both methods in the 1 - 30 μg/mL range (r > 0.9999). The specificity tests showed that there was no interference of the NP components on the quantitative analyses. Precision (repeatability and intermediate precision) was demonstrated by a relative standard deviation lower than 1.5%, whereas the accuracy was assessed by the recovery of MTX from sample matrices, given mean value of ~99%. The proposed methods were applied for the analyses of MTX in different batches of NPs, and the results showed non-significant differences (p > 0.05) between the values obtained with both methodologies. Moreover, the RP-LC method was successfully used to determine the drug entrapment efficiency, and to quantify MTX during in vitro release assays and photolytic degradation studies. In conclusion, the validated methods are suitable to assay MTX in pH-sensitive CS-NPs without any interference from the polymer or surfactant.Pharma Publisher2016201620142016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion9 p.application/pdfhttps://hdl.handle.net/2445/97209Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.14205/2309-4435.2014.02.02.3Journal of Applied Biopharmaceutics and Pharmacokinetics , 2014, vol. 2, num. 2, p. 59-67http://dx.doi.org/10.14205/2309-4435.2014.02.02.3cc-by-nc (c) Nogueira D.R. et al., 2014http://creativecommons.org/licenses/by-nc/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/972092026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Determination of Methotrexate in pH-Sensitive Chitosan Nanoparticles by Validated RP-LC and UV Spectrophotometric Methods |
| title |
Determination of Methotrexate in pH-Sensitive Chitosan Nanoparticles by Validated RP-LC and UV Spectrophotometric Methods |
| spellingShingle |
Determination of Methotrexate in pH-Sensitive Chitosan Nanoparticles by Validated RP-LC and UV Spectrophotometric Methods Nogueira, Daniele R. Quitosan Nanopartícules Medicaments antineoplàstics Metotrexat Agents tensioactius Cromatografia Espectroscòpia Chitosan Nanoparticles Antineoplastic agents Methotrexate Surface active agents Chromatography Spectrum analysis |
| title_short |
Determination of Methotrexate in pH-Sensitive Chitosan Nanoparticles by Validated RP-LC and UV Spectrophotometric Methods |
| title_full |
Determination of Methotrexate in pH-Sensitive Chitosan Nanoparticles by Validated RP-LC and UV Spectrophotometric Methods |
| title_fullStr |
Determination of Methotrexate in pH-Sensitive Chitosan Nanoparticles by Validated RP-LC and UV Spectrophotometric Methods |
| title_full_unstemmed |
Determination of Methotrexate in pH-Sensitive Chitosan Nanoparticles by Validated RP-LC and UV Spectrophotometric Methods |
| title_sort |
Determination of Methotrexate in pH-Sensitive Chitosan Nanoparticles by Validated RP-LC and UV Spectrophotometric Methods |
| dc.creator.none.fl_str_mv |
Nogueira, Daniele R. Macedo, Letícia B. Scheeren, Laís E. Mitjans Arnal, Montserrat Infante Martínez-Pardo, Ma. Rosa Rolim, Clarice M. B. Vinardell Martínez-Hidalgo, Ma. Pilar |
| author |
Nogueira, Daniele R. |
| author_facet |
Nogueira, Daniele R. Macedo, Letícia B. Scheeren, Laís E. Mitjans Arnal, Montserrat Infante Martínez-Pardo, Ma. Rosa Rolim, Clarice M. B. Vinardell Martínez-Hidalgo, Ma. Pilar |
| author_role |
author |
| author2 |
Macedo, Letícia B. Scheeren, Laís E. Mitjans Arnal, Montserrat Infante Martínez-Pardo, Ma. Rosa Rolim, Clarice M. B. Vinardell Martínez-Hidalgo, Ma. Pilar |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
Quitosan Nanopartícules Medicaments antineoplàstics Metotrexat Agents tensioactius Cromatografia Espectroscòpia Chitosan Nanoparticles Antineoplastic agents Methotrexate Surface active agents Chromatography Spectrum analysis |
| topic |
Quitosan Nanopartícules Medicaments antineoplàstics Metotrexat Agents tensioactius Cromatografia Espectroscòpia Chitosan Nanoparticles Antineoplastic agents Methotrexate Surface active agents Chromatography Spectrum analysis |
| description |
Nanotechnology-based drug delivery systems are in constant development and, therefore, it is of great importance to have rapid, efficient and accurate analytical methodology to quantify the encapsulated drugs. Here, simple and fast methods, by reversed-phase liquid chromatography (RP-LC) and UV spectrophotometry, were developed and validated for the determination of methotrexate (MTX) in pH-sensitive chitosan nanoparticles (CS-NPs). NPs were prepared using a modified ionotropic complexation process, in which was included a surfactant derived from Nα,Nε-dioctanoyl lysine with an inorganic sodium counterion. The RP-LC method was carried out on a Waters XBridgeTM C18 column (250 mm x 4.6 mm I.D., 5μm), with mobile phase consisted of potassium phosphate buffer (0.05 M, pH 3.2): acetonitrile (86:14, v/v), and UV detection set at 303 nm. The analyses of MTX content by the UV method were also accomplished at 303 nm, using 0.1 M sodium hydroxide as diluent. The measurements were linearly correlated with concentration for both methods in the 1 - 30 μg/mL range (r > 0.9999). The specificity tests showed that there was no interference of the NP components on the quantitative analyses. Precision (repeatability and intermediate precision) was demonstrated by a relative standard deviation lower than 1.5%, whereas the accuracy was assessed by the recovery of MTX from sample matrices, given mean value of ~99%. The proposed methods were applied for the analyses of MTX in different batches of NPs, and the results showed non-significant differences (p > 0.05) between the values obtained with both methodologies. Moreover, the RP-LC method was successfully used to determine the drug entrapment efficiency, and to quantify MTX during in vitro release assays and photolytic degradation studies. In conclusion, the validated methods are suitable to assay MTX in pH-sensitive CS-NPs without any interference from the polymer or surfactant. |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014 2016 2016 2016 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/97209 |
| url |
https://hdl.handle.net/2445/97209 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: http://dx.doi.org/10.14205/2309-4435.2014.02.02.3 Journal of Applied Biopharmaceutics and Pharmacokinetics , 2014, vol. 2, num. 2, p. 59-67 http://dx.doi.org/10.14205/2309-4435.2014.02.02.3 |
| dc.rights.none.fl_str_mv |
cc-by-nc (c) Nogueira D.R. et al., 2014 http://creativecommons.org/licenses/by-nc/3.0/es info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
cc-by-nc (c) Nogueira D.R. et al., 2014 http://creativecommons.org/licenses/by-nc/3.0/es |
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openAccess |
| dc.format.none.fl_str_mv |
9 p. application/pdf |
| dc.publisher.none.fl_str_mv |
Pharma Publisher |
| publisher.none.fl_str_mv |
Pharma Publisher |
| dc.source.none.fl_str_mv |
Articles publicats en revistes (Bioquímica i Fisiologia) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Recercat. Dipósit de la Recerca de Catalunya |
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Recercat. Dipósit de la Recerca de Catalunya |
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15,811543 |